Home » Target » Rhodopsin antibody

Rhodopsin antibody

Principal name

Rhodopsin antibody

Alternative names for Rhodopsin antibody

RHO, Opsin-2, OPN2

SwissProt ID

O16005 (Sepof), O16017 (Camlu), O16018 (Camsc), O16019 (Orcvi), O16020 (Proml), O18312 (Camhu), O18315 (Camma), O18481 (Orcau), O18485 (Proor), O18486 (Prose), O18766 (Pig), O42268 (Ictpu), O42294 (Abyko), O42300 (Batmu), O42301 (Batni), O42307 (Cotbo), O42327 (Comdy), O42328 (Cotgr), O42330 (Cotin), O42427 (Limbe), O42431 (Limpa), O42451 (Proje), O42452 (Parkn), O42466 (Taubu), O42604 (Zeufa), O62791 (Delde), O62792 (Glome), O62793 (Mesbi), O62794 (Phovi), O62795 (Paggo), O62796 (Trima), O62798 (Turtr), O93441 (Galml), O93459 (Scyca), P02699 (Bovin), P02700 (Sheep), P08100 (Human), P09241 (Entdo), P15409 (Mouse), P22328 (Chick), P22671 (Lamja), P24603 (Lolfo), P28681 (Crigr), P29403 (Xenla), P31355 (Ranpi), P31356 (Todpa), P32308 (Canfa), P32309 (Carau), P35356 (Procl), P35359 (Danre), P35362 (Sphsp), P35403 (Pommi), P41590 (Astfa), P41591 (Anoca), P49912 (Rabit), P51470 (Ranca), P51488 (Cypca), P51489 (Rat), P52202 (Allmi), P56514 (Bufbu), P56515 (Bufma), P56516 (Rante), P79756 (Gamaf), P79798 (Myrbe), P79807 (Myrvi), P79808 (Neoar), P79809 (Neoau), P79812 (Neosa), P79848 (Poere), P79863 (Leuer), P79898 (Sardi), P79901 (Sarmi), P79902 (Sarpu), P79903 (Sarsp), P79911 (Sarti), P79914 (Sarxa), P87369 (Oryla), Q17053 (Apime), Q17094 (Lolsu), Q17292 (Camab), Q17296 (Catbo), Q28886 (Macfa), Q68J47 (Loxaf), Q6W3E1 (Calpd), Q769E8 (Otocr), Q8HY69 (Smicr), Q90245 (Ambti), Q90305 (Corau), Q90373 (Cotke), Q95KU1 (Felca), Q98980 (Petma), Q9DGG4 (Tetng), Q9YGY9 (Zosop), Q9YGZ0 (Sarpi), Q9YGZ1 (Athbo), Q9YGZ2 (Gobni), Q9YGZ3 (Salpv), Q9YGZ4 (Dicla), Q9YGZ5 (Solso), Q9YGZ6 (Lizau), Q9YGZ7 (Lizsa), Q9YGZ8 (Chelb), Q9YGZ9 (Mugce), Q9YH00 (Litmo), Q9YH01 (Mulsu), Q9YH02 (Spaau), Q9YH03 (Sarsl), Q9YH04 (Dipvu), Q9YH05 (Dipan)

Gene ID

6010 (RHO), 24717 (Rho), 509933

Ncbi ID

NP_000530.1, 9606, 10116, CAL37658.1, NM_000539, NP_001014890.1, NM_033441

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mam (Mammalia), Av (Birds), Amph (Amphibian), Bov (Bovine), Fi (Fish), Frog, Por (Porcine), Ze (Zebrafish), Ve (Vertebrates), Fe (Feline), Mky (Monkey), Rb (Rabbit), Can (Canine), GP (Guinea Pig), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Rhodopsin antibody

Rhodopsin is the protein in the mammalian reti responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the Rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate sigling on binding a specific ligand, Rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retil. Retil can exist in two isomeric forms, 11-cis and 11-trans retil. In the dark Rhodopsin is associated with 11-cis retil, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the Rhodopsin making it catalytically active. Activated Rhodopsin in turn activates the GTP binding protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an alpha and a beta gamma subunit. The alpha subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolysescyclic GMP. This in turn results in the closure of cyclic nucleotide gated ion channels in the rod cell membrane, which prevent the influx of sodium and calcium ions. This results in an increase in the membrane potential of the rod cell and so reduces the rate of syptic sigling. So light stimulation results in a reduced rate of photoreceptor syptic release. This information is transmitted through neurons of the reti to the visual centers of the brain. Rhodopsin activity is shut off by phosphorylation under the influence of Rhodopsin kise, the activity of which results in binding of visual arrestin, which prevents Rhodopsin from interacting with and activating more transducin molecules. This basic sigling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kise are found in these pathways.

Immunohistochemistry (IHC) analyzes of Rhodopsin antibody (Cat.-No.: AP06577PU-N) in paraffin-embedded human brain tissue.

AP06577PU-N

Western blot (WB) analyzes of Rhodopsin antibody (Cat.-No.: AP06577PU-N) in extracts from COLO205 cells.

AP06577PU-N

The cell lysate derived from MCF-7 was immunoprobed by RHO polyclonal antibody ( Cat # PAB12727 ) at 1 : 500. An immunoreactive band is observed around ~24 kDa ( 2 ). This band is abolished by pre-incubation with immunizing peptide ( 1 ).

PAB12727

Figure 1. Immunohistochemical staining of mouse retinal section showing specific immunolabeling of the rhodopsin protein in the rod spherules. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.

AM08266PU-N

Immunofluorescence of Rho using a dilution of Rhodopsin antibody

Western blot analysis of Rhodopsin in human cell lines using a 1:1000 dilution of anti-RHO.

Immunohistochemistry analysis of paraffin-embedded human brain, using Rhodopsin (Phospho-Ser334) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Rhodopsin (Ab-334) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COLO cells, using Rhodopsin (Ab-334) Antibody. The lane on the right is treated with the synthesized peptide.

Human Placenta; WB Suggested Anti-RHO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Placenta; RHO antibody - C-terminal region (ARP59909_P050) in Human Placenta cells using Western Blot

ARP59909_P050

Immunohistochemical staining of mouse retinal section showing specific immunolabeling of the rhodopsin protein in the rod spherules. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.

TA309233

Western blot analysis of extracts from A549 cells, using RHO antibody.The lane on the right is treated with the synthesized peptide.

TA311787

Western Blot: Rhodopsin Antibody (A531) [TA337112] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hy

TA337112

Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (A531) [TA337112] - Pig retinal section stained with TA337112 (green) and counterstained with rabbit polyclonal antibody to NF-M NB300-133 (red) and DNA (blue). Rhodopsin is most abundant in the o

TA337112

Western Blot: Rhodopsin Antibody (B630) [TA337113] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hy

TA337113

Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (B630) [TA337113] - High magnification confocal image of pig retinal section stained with TA337113 (Green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized

TA337113

Anti-RHO / Rhodopsin antibody IHC of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340572

WB Suggested Anti-RHO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Placenta

TA342775

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA309470	Rhodopsin (C-term) antibody
	Mouse Monoclonal anti-RHO Antibody
	Mouse	IgG1	1D4	Purified	Ve, Vertebrates.	C, E, ICC/IF, IP, WB	0.1 mg / €410.00
							OriGene Technologies, Inc.
	Published recently!
TA309233	Rhodopsin antibody
	Mouse Anti-Rhodopsin Antibody
	Mouse	IgG1	1D4	Purified	Hu, Ms, Rt	P, WB	0.1 ml / €405.00
							OriGene Technologies, Inc.
TA309469	Rhodopsin (N-term) antibody
	Mouse Monoclonal anti-RHO Antibody
	Mouse	IgG1	4D2	Purified	Amph, Bov, Fi, Frog, Hu, Mam, Ms, Por, Rt, Ze	C, E, ICC/IF, IP, WB	0.1 mg / €410.00
							OriGene Technologies, Inc.
TA311787	Rhodopsin antibody
	Rabbit polyclonal anti-RHO (rhodopsin) antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA337112	Rhodopsin antibody
	Mouse Monoclonal Rhodopsin Antibody (A531)
	Mouse	IgG1	A531	Purified	Bov, Hu, Ms, Por, Rt	ICC/IF, WB	0.1 ml / €395.00
							OriGene Technologies, Inc.
+1 additional image
TA337113	Rhodopsin antibody
	Mouse Monoclonal Rhodopsin Antibody (B630)
	Mouse	IgG1	B630	Purified	Bov, Hu, Ms, Por, Rt	ICC/IF, WB	0.1 ml / €395.00
							OriGene Technologies, Inc.
+1 additional image
TA340572	Rhodopsin (2nd extracell. dom.) antibody
	Rabbit Polyclonal Anti-RHO Antibody (Extracellular Domain)
	Rabbit			Aff - Purified	Elephant, Fe, Hu, Mky, Ms, Por, Rb, Rt, Ze	P	50 µg / €425.00
							OriGene Technologies, Inc.
AP06577PU-N	Rhodopsin antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image

Lysates

Catalog No.
LY400184	RHO overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR211712L2V	Lenti ORF particles, Rho (GFP-tagged ORF) - Rat rhodopsin (Rho), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR211712L1V	Lenti ORF particles, Rho (Myc-DDK-tagged ORF) - Rat rhodopsin (Rho), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

Antibodies (see all»)

Kits (see all»)

Antigens

Lysates (see all»)

Tissue Slides (see all»)

Other life-science products