Rhombotin-2 antibody
Principal name
Rhombotin-2 antibody
Alternative names for Rhombotin-2 antibody
LMO2, RBTN2, RBTNL1, RHOM2, TTG2, Rhombotin2, LIM domain only protein 2, Cysteine-rich protein TTG-2, T-cell translocation protein 2
Ncbi ID
9606, NP_001135787.1, NP_001135787, NP_005565, NP_005565.1, NM_001142315, NM_001142316, NP_001135788, NP_001135808.1, NM_008505, NM_001142337, NM_001142335
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), ChIP assay (ChIP)
Background of Rhombotin-2 antibody
LMO2 (LIM-only protein 2) is a nuclear transcriptiol regulator essential for normal blood cell development and it exerts its effects by mediating protein-protein interactions as well as by nucleating multicomponent transcriptiol complexes. LMO2 interacts with LDB1 (LIM domain binding protein 1) through the tandem LIM domains of LMO2 and LID (LIM interaction domain) of LDB1. LMO2 also interacts with bHlH proteins TAL1/SCL, BEX2 and KDM5A, and form complex with TAL1/SCL. It functions with TAL1/SCL in the regulation of RBCs development and with LDB1 to maintain erythroid precursors in immature state. LMO2 has been associated with the maintence of hematopoietic stem cells, RBCs differentiation, and angiogenesis in both normal development as well as during carcinogenesis. Disruption of LMO2 in mice causes death in midway through embryonic development as they do not develop RBCs. Excessive expression of LMO2 in T-cells, through either chromosomal translocations or retroviral insertion of gene therapy vectors, as well as transgenic overexpression of LMO2 in mouse model leads to the onset of T-cell acute lymphoblastic leukemia (T-ALL).
LMO2 monoclonal antibody (M01), clone 6B8 Western Blot analysis of LMO2 expression in HL-60 ( Cat # L014V1 ).
LMO2 monoclonal antibody (M02), clone 2B3. Western Blot analysis of LMO2 expression in human spleen.
LMO2 monoclonal antibody (M02), clone 2B3 Western Blot analysis of LMO2 expression in HL-60 ( Cat # L014V1 ).
LMO2 monoclonal antibody (M05), clone 4D3. Western Blot analysis of LMO2 expression in human spleen.
Western Blot analysis of LMO2 expression in transfected 293T cell line (H00004005-T01) by LMO2 MaxPab polyclonal antibody.Lane 1: LMO2 transfected lysate(18.40 KDa).Lane 2: Non-transfected lysate.
AM11170PU LMO2 antibody staining of Human tonsil.
Human tonsil stained with Anti-LMO2
Immunohistochemical staining of LMO2 on formalin fixed, paraffin embedded human tonsil with LMO2 monoclonal antibody, clone 1A9-3D11.
Immunofluorescent labeling of LMO2 ( green ) on human tonsil tissue using LMO2 monoclonal antibody, clone 1A9-3B11 ( Cat #MAB2336 ).
Western Blot Analysis of LMO2 in Ramos cell lysate using LMO2 monoclonal antibody, clone 1A9-3B11 ( Cat #MAB2336 ).
Immunohistochemical staining of LMO2 on formalin fixed, paraffin embedded human tonsil with LMO2 polyclonal antibody.
Western blot analysis of LMO2 in Ramos whole cell lysate using LMO2 polyclonal antibody ( Cat # PAB12404 ).
Western Blot analysis of Ramos cell lysate with LMO2 antibody
Flow cytometric analysis of Rabbit anti-LMO2 Antibody Cat.-No AM09341PU (Clone SP51) in RAMOS (green) compare to negative control of rabbit IgG (blue) .
Human tonsil stained with anti-LMO2 Antibody Cat.-No AM09341PU (Clone SP51).
Human kidney; WB Suggested Anti-LMO2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human kidney; LMO2 antibody - N-terminal region (P100952_P050) in Human kidney cells using Western Blot
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry of Human tonsil tissue at a 1/1000 dilution of anti-Human LMO2 antibody followed by Biotin anti-mouse IgG and HRP-conjugated avidin. Low (left) and high (right) magnifications are shown.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506132)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506132)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506132)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506132)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506132)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506132)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506132)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506132)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506132)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMO2 (RC205376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMO2.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506133)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506133)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506133)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMO2 (RC205376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMO2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506139)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506139)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LMO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506139)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMO2 (RC205376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMO2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMO2 (RC205376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMO2.
Western Blot: LMO2 Antibody (1A9-3B11) - Detection of LMO2 in Ramos cell lysate
Immunofluorescence: LMO2 Antibody (1A9-3B11) - Immunofluorescent labeling of LMO2 (green) in tonsil tissue.
Immunohistochemistry of human tonsil tissue at a 1:1000 dilution of Anti-Human LMO2 Purified followed by Anti-Mouse IgG Biotin and avidin HRP and high (right) magnifications are shown.
WB Suggested Anti-LMO2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human kidney
SDS-Page: LMO2 Antibody [TA336557] - Detection of LMO2 in Ramos whole cell lysate using TA336557.
Host: Rabbit; Target Name: LMO2; Sample Tissue: Ovary Tumor lysates; Antibody Dilution: 1.0ug/ml
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA506132 | Rhombotin-2 antibody |
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| LMO2 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10) | |||||||
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Mouse | IgG1 | OTI1G10 | Purified | Hu | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +9 additional images |
Highly validated antibody!
|
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| TA506133 | Rhombotin-2 antibody |
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| LMO2 mouse monoclonal antibody, clone OTI1D7 (formerly 1D7) | |||||||
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Mouse | IgG1 | OTI1D7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA506139 | Rhombotin-2 antibody |
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| LMO2 mouse monoclonal antibody, clone OTI1C8 (formerly 1C8) | |||||||
 |
Mouse | IgG1 | OTI1C8 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA309733 | Rhombotin-2 antibody |
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| Mouse Monoclonal LMO2 Antibody (1A9-3B11) | |||||||
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Mouse | IgG1 | 1A9-3B11 | Purified | Hu | C, ICC/IF, IP, WB |
0.1 ml /
€415.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA320413 | Rhombotin-2 antibody |
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| Mouse Anti-Human LMO2 Purified (25 ug) | |||||||
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Mouse | 1A9-3B11 | Purified | Hu | P |
25 µg /
€195.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA336557 | Rhombotin-2 antibody |
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| Rabbit Polyclonal LMO2 Antibody | |||||||
![SDS-Page: LMO2 Antibody [TA336557] - Detection of LMO2 in Ramos whole cell lysate using TA336557.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB110-83978-1-W.jpg) |
Rabbit | Purified | Bov, Hu, Ms, Rt | ChIP, WB |
0.1 ml /
€450.00
|
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| OriGene Technologies, Inc. | |||||||
| TA506177 | Rhombotin-2 antibody |
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| LMO2 mouse monoclonal antibody, clone OTI2D7 (formerly 2D7) | |||||||
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Mouse | IgG1 | OTI2D7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AM20401PU-N | Rhombotin-2 antibody |
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Mouse | IgG1 | 1A9-3B11 | Aff - Purified | Hu | P |
50 µg /
€425.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP05031PU-N | Rhombotin-2 antibody |
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Sheep | IgG | Purified | Hu | WB |
0.25 mg /
€400.00
|
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305376 | Rhombotin-2 (transcript variant 1) |
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| Recombinant protein of human LIM domain only 2 (rhombotin-like 1) (LMO2), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP326762 | Rhombotin-2 (transcript variant 2) |
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| Recombinant protein of human LIM domain only 2 (rhombotin-like 1) (LMO2), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP326864 | Rhombotin-2 (transcript variant 3) |
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| Recombinant protein of human LIM domain only 2 (rhombotin-like 1) (LMO2), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP329124 | Rhombotin-2 (transcript variant 1) |
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| Recombinant protein of human LIM domain only 2 (rhombotin-like 1) (LMO2), transcript variant 1. | |||||
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Human | > 80 % Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY428033 | LMO2 overexpression lysate |
|
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0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. | ||
| LY428034 | LMO2 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |