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RICTOR antibody

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Principal name

RICTOR antibody

Alternative names for RICTOR antibody

KIAA1999, Rapamycin-insensitive companion of mTOR, mAVO3

SwissProt ID

Q6QI06 (Mouse), Q6R327 (Human)

Gene ID

253260 (RICTOR), 78757 (Rictor)

Ncbi ID

NP_689969, NP_689969.2, 9606, NP_084444.3, NM_152756

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Bov (Bovine), Can (Canine), Eq (Equine), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Bear

Available hosts

Mouse, Goat, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of RICTOR antibody

Kit Component:
- KN314817G1, 4921505C17Rik gRNA vector 1 in pCas-Guide vector
- KN314817G2, 4921505C17Rik gRNA vector 2 in pCas-Guide vector
- KN314817D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Sarbassov dos D, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptorindependent pathway that regulates the cytoskeleton. Curr Biol. 2004 Jul 27;14(14):1296-302.

AM20222PU-N - RICTOR
Detection of endogenous Rictor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied toSDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20222PU-N RICTOR antibody (Clone 1G11) at 0.5 µg/ml for 1h at RTand developed by ECL (exp. time: 30 sec).Lane 1: HeLaLane 2: HepG2Lane 3: HEK293Lane 4: SH-SY5YLane 5: MDCKLane 6: PC12Lane 7: CMT 93 Lane 8: Neuro 2ALane 9: 3T3
AM31901PU-N - RICTOR
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31901PU-N - RICTOR
Western Blot analysis of RICTOR expression in in transfected 293T cell using RICTOR monoclonal antibody clone 1F3 Cat.-No AM31901PU-N.
AM31901PU-N - RICTOR
Western Blot analysis of RICTOR expression in Hela NE using RICTOR monoclonal antibody clone 1F3 Cat.-No AM31901PU-N.
AM31901PU-N - RICTOR
Detection limit for recombinant GST tagged RICTOR is approximately 0.03ng/ml as a capture antibody.
AM31901PU-N - RICTOR
Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR Rabbit polyclonal antibody.
AP07846PU-N - RICTOR
Figure 1. Staining RICTOR in Renal Tubular Epithelium by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
AP13961PU-N - RICTOR
Formalin-fixed and paraffin-embedded human Lymph tissue reacted with Rictor antibody (N-term)(AP13961PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP13964PU-N - RICTOR
Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR antibody (N-term) (AP13964PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP13964PU-N - RICTOR
Western blot analysis of anti-RICTOR Pab (AP13964PU-N) in SK-BR-3 cell line lysates (35 µg/lane). RICTOR (arrow) was detected using the purified Pab (1:60 dilution).
H00253260-B01 - RICTOR
Western Blot analysis of RICTOR expression in transfected 293T cell line (H00253260-T01) by RICTOR MaxPab polyclonal antibody.Lane 1: RICTOR transfected lysate(29.15 KDa).Lane 2: Non-transfected lysate.
H00253260-D01P - RICTOR
Proximity Ligation Analysis (PLA) of protein-protein interactions between RICTOR and RPS6KB1 HeLa cells were stained with anti-RICTOR rabbit purified polyclonal 1:1200 and anti-RPS6KB1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
H00253260-D01P - RICTOR
Western Blot analysis of RICTOR expression in transfected 293T cell line (H00253260-T02) by RICTOR MaxPab polyclonal antibody.Lane 1: RICTOR transfected lysate(29.80 KDa).Lane 2: Non-transfected lysate.
H00253260-M01 - RICTOR
Immunoperoxidase of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
H00253260-M01 - RICTOR
RICTOR monoclonal antibody (M01), clone 1F3 Western Blot analysis of RICTOR expression in Hela NE ( Cat # L013V3 ).
H00253260-M01 - RICTOR
Detection limit for recombinant GST tagged RICTOR is approximately 0.03ng/ml as a capture antibody.
PAB2053 - RICTOR
Formalin-fixed and paraffin-embedded human Lymph tissue reacted with RICTOR polyclonal antibody ( Cat # PAB2053 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
PAB2054 - RICTOR
Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR polyclonal antibody ( Cat # PAB2054 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
PAB2054 - RICTOR
Western blot analysis of RICTOR polyclonal antibody ( Cat # PAB2054 ) in SK-BR-3 cell line lysates ( 35 µg/lane ) .RICTOR ( arrow ) was detected using the purified Polyclonal antibody ( 1 : 60 dilution ) .
PAB6780 - RICTOR
RICTOR polyclonal antibody ( Cat # PAB6780 ) ( 2.5 µg/ml ) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
AM06499SU-N - RICTOR
Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and Human breast carcinoma (right) using RICTOR antibody Cat.-No AM06499SU-N with DAB staining.
AM06499SU-N - RICTOR
Immunofluorescence analysis of NIH/3T3 cells using RICTOR antibody Cat.-No AM06499SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
AM06499SU-N - RICTOR
Western blot analysis using RICTOR antibody Cat.-No AM06499SU-N against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.
AM06499SU-N - RICTOR
Flow Cytometric analysis of Hela cells using RICTOR antibody Cat.-No AM06499SU-N (green) and negative control (purple).
AM06502SU-N - RICTOR
Western blot analysis using RICTOR antibody Cat.-No AM06502SU-N against Hela (1), PANC-1 (2), MOLT4 (3), and HepG2 (4) cell lysate.
TA303018 - RICTOR
TA303018 (2.5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
TA336802 - RICTOR
Western Blot: RICTOR Antibody (7B3) [TA336802] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates.
TA336802 - RICTOR
Immunohistochemistry-Paraffin: RICTOR Antibody (7B3) [TA336802] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining.
TA336802 - RICTOR
Immunocytochemistry/Immunofluorescence: RICTOR Antibody (7B3) [TA336802] - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
TA336802 - RICTOR
Flow Cytometry: RICTOR Antibody (7B3) [TA336802] - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple).
TA336802 - RICTOR
Flow Cytometry: RICTOR Antibody (7B3) [TA336802] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used
TA308477 - RICTOR
Sample(30 ug whole cell lysate). A: A431 . 5% SDS PAGE. TA308477 diluted at 1:1000
TA308477 - RICTOR
Staining of Human Kidney cancer tissue sections using anti-Rictor antibody (TA308477).
TA308477 - RICTOR
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RICTOR(TA308477) antibody at 1:200 dilution.
TA319016 - RICTOR
Western blot analysis of Rictor with lysate from Jurkat cells (Lane 1) and Hela cells (Lane 2).
TA349591 - RICTOR
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (RICTOR Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
TA349591 - RICTOR
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (RICTOR Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RICTOR (C-term) antibody
  Goat Polyclonal Antibody against RICTOR  
TA303018 (2.5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Can, Hu, Ms P
0.1 mg / €360.00
  OriGene Technologies, Inc.

RICTOR antibody
  Rabbit polyclonal antibody to RICTOR  
Sample(30 ug whole cell lysate). A: A431 . 5% SDS PAGE. TA308477 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

RICTOR antibody
  Rabbit polyclonal anti-Rictor antibody  
Western blot analysis of Rictor with lysate from Jurkat cells (Lane 1) and Hela cells (Lane 2). Rabbit Purified Hu, Ms, Rt WB
0.2 mg / €355.00
  OriGene Technologies, Inc.

RICTOR antibody
  Rabbit Polyclonal Anti-RICTOR Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (RICTOR Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

RICTOR (C-term) antibody
Figure 1. Staining RICTOR in Renal Tubular Epithelium by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Goat Aff - Purified Blood fluke, Can, Elephant, Eq, Hu, Mky E, P
50 µg / €425.00
  OriGene Technologies GmbH

RICTOR (N-term) antibody
Formalin-fixed and paraffin-embedded human Lymph tissue reacted with Rictor antibody (N-term)(AP13961PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P
0.4 ml / €415.00
  OriGene Technologies GmbH

RICTOR (N-term) antibody
Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR antibody (N-term) (AP13964PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

7 Item(s)

per page

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Product Type
  1. Primary Antibody (7)
Target
  1. RICTOR (7)
Property
  1. C-term (2)
  2. N-term (2)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (3)
  3. Canine (Can) (2)
  4. Blood fluke (1)
  5. Elephant (1)
  6. Equine (Eq) (1)
  7. Monkey (Mky) (1)
  8. Rat (Rt) (1)
Applications
  1. Paraffin Sections (P) (6)
  2. Western blot / Immunoblot (WB) (3)
  3. Enzyme Immunoassay (E) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
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  2. Aff - Purified (3)
Host
  1. Rabbit (5)
  2. Goat (2)
Images
  1. 1 image (7)
  2. 3+ images (1)
Images: Applications
  1. IHC (3)
  2. Paraffin Sections (P) (3)
  3. Western blot / Immunoblot (WB) (3)
  4. Immunofluorescence (IF) (1)
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