RICTOR antibody
Principal name
RICTOR antibody
Alternative names for RICTOR antibody
KIAA1999, Rapamycin-insensitive companion of mTOR, mAVO3
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)
Background of RICTOR antibody
- Kit Component:
- - KN314817G1, 4921505C17Rik gRNA vector 1 in pCas-Guide vector
- KN314817G2, 4921505C17Rik gRNA vector 2 in pCas-Guide vector
- KN314817D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Sarbassov dos D, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptorindependent pathway that regulates the cytoskeleton. Curr Biol. 2004 Jul 27;14(14):1296-302.
Detection of endogenous Rictor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied toSDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20222PU-N RICTOR antibody (Clone 1G11) at 0.5 µg/ml for 1h at RTand developed by ECL (exp. time: 30 sec).Lane 1: HeLaLane 2: HepG2Lane 3: HEK293Lane 4: SH-SY5YLane 5: MDCKLane 6: PC12Lane 7: CMT 93 Lane 8: Neuro 2ALane 9: 3T3
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of RICTOR expression in in transfected 293T cell using RICTOR monoclonal antibody clone 1F3 Cat.-No AM31901PU-N.
Western Blot analysis of RICTOR expression in Hela NE using RICTOR monoclonal antibody clone 1F3 Cat.-No AM31901PU-N.
Detection limit for recombinant GST tagged RICTOR is approximately 0.03ng/ml as a capture antibody.
Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR Rabbit polyclonal antibody.
Figure 1. Staining RICTOR in Renal Tubular Epithelium by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Formalin-fixed and paraffin-embedded human Lymph tissue reacted with Rictor antibody (N-term)(AP13961PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR antibody (N-term) (AP13964PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-RICTOR Pab (AP13964PU-N) in SK-BR-3 cell line lysates (35 µg/lane). RICTOR (arrow) was detected using the purified Pab (1:60 dilution).
Western Blot analysis of RICTOR expression in transfected 293T cell line (H00253260-T01) by RICTOR MaxPab polyclonal antibody.Lane 1: RICTOR transfected lysate(29.15 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between RICTOR and RPS6KB1 HeLa cells were stained with anti-RICTOR rabbit purified polyclonal 1:1200 and anti-RPS6KB1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of RICTOR expression in transfected 293T cell line (H00253260-T02) by RICTOR MaxPab polyclonal antibody.Lane 1: RICTOR transfected lysate(29.80 KDa).Lane 2: Non-transfected lysate.
Immunoperoxidase of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
RICTOR monoclonal antibody (M01), clone 1F3 Western Blot analysis of RICTOR expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged RICTOR is approximately 0.03ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human Lymph tissue reacted with RICTOR polyclonal antibody ( Cat # PAB2053 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR polyclonal antibody ( Cat # PAB2054 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of RICTOR polyclonal antibody ( Cat # PAB2054 ) in SK-BR-3 cell line lysates ( 35 µg/lane ) .RICTOR ( arrow ) was detected using the purified Polyclonal antibody ( 1 : 60 dilution ) .
RICTOR polyclonal antibody ( Cat # PAB6780 ) ( 2.5 µg/ml ) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and Human breast carcinoma (right) using RICTOR antibody Cat.-No AM06499SU-N with DAB staining.
Immunofluorescence analysis of NIH/3T3 cells using RICTOR antibody Cat.-No AM06499SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using RICTOR antibody Cat.-No AM06499SU-N against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.
Flow Cytometric analysis of Hela cells using RICTOR antibody Cat.-No AM06499SU-N (green) and negative control (purple).
Western blot analysis using RICTOR antibody Cat.-No AM06502SU-N against Hela (1), PANC-1 (2), MOLT4 (3), and HepG2 (4) cell lysate.
TA303018 (2.5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: RICTOR Antibody (7B3) [TA336802] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates.
Immunohistochemistry-Paraffin: RICTOR Antibody (7B3) [TA336802] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining.
Immunocytochemistry/Immunofluorescence: RICTOR Antibody (7B3) [TA336802] - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry: RICTOR Antibody (7B3) [TA336802] - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple).
Flow Cytometry: RICTOR Antibody (7B3) [TA336802] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used
Sample(30 ug whole cell lysate). A: A431 . 5% SDS PAGE. TA308477 diluted at 1:1000
Staining of Human Kidney cancer tissue sections using anti-Rictor antibody (TA308477).
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RICTOR(TA308477) antibody at 1:200 dilution.
Western blot analysis of Rictor with lysate from Jurkat cells (Lane 1) and Hela cells (Lane 2).
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (RICTOR Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (RICTOR Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP07846PU-N | RICTOR (C-term) antibody |
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Goat | Aff - Purified | Blood fluke, Can, Elephant, Eq, Hu, Mky | E, P |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
| AP13961PU-N | RICTOR (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
| AP13964PU-N | RICTOR (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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