RIOK2 antibody
Principal name
RIOK2 antibody
Alternative names for RIOK2 antibody
RIO kinase 2, Serine/threonine-protein kinase RIO2
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Immunoprecipitation (IP), Flow Cytometry (F)
Background of RIOK2 antibody
- Kit Component:
- - KN314838G1, Riok2 gRNA vector 1 in pCas-Guide vector
- KN314838G2, Riok2 gRNA vector 2 in pCas-Guide vector
- KN314838D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of RIOK2 polyclonal antibody ( Cat # PAB4521 ) in CEM cell line lysate ( 35 µg/lane ) . RIOK2 ( arrow ) was detected using the purified RIOK2 polyclonal antibody ( Cat # PAB4521 ) ( 1 : 60 dilution ) .
This Western blot recognizes the derivative of the full length RIOK2 . The accession number of the full length RIOK2 is : NP_060813 ; Q9BVS4 . Western blot analysis of RIOK2 polyclonal antibody ( Cat # PAB4522 ) in Jurkat cell line lysates ( 35 µg/lane ) . RIOK2 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
This Western blot recognizes the derivative of the full length RIOK2 . The accession number of the full length RIOK2 is : NP_060813 ; Q9BVS4 . Western blot analysis of RIOK2 polyclonal antibody ( Cat # PAB4522 ) in T-47D cell line lysates ( 35 µg/lane ) . RIOK2 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Immunoperoxidase of monoclonal antibody to RIOK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to RIOK2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of RIOK2 expression in transfected 293T cell line (H00055781-T02) by RIOK2 MaxPab polyclonal antibody.Lane 1: RIOK2 transfected lysate(60.72 KDa).Lane 2: Non-transfected lysate.
RIOK2 MaxPab polyclonal antibody. Western Blot analysis of RIOK2 expression in COLO 320 HSR.
Western blot analysis of anti-RIOK2 Antibody (C-term) in Ramos cell line lysates (35ug/lane). RIOK2(arrow) was detected using the purified Pab.
This Western blot recognizes the derivative of the full length RIOK2. Western blot analysis of anti-RIOK2 Pab in Jurkat(left) and T47D(right) cell line lysates (35ug/lane). RIOK2(arrow) was detected using the purified Pab (1:60 dilution).
Western Blot analysis of RIOK2 expression in transfected 293T cell line (H00055781-T02) by RIOK2 MaxPab polyclonal antibody.Lane 1: RIOK2 transfected lysate(63.30 KDa).Lane 2: Non-transfected lysate.
Human Heart; WB Suggested Anti-RIOK2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human heart; RIOK2 antibody - N-terminal region (ARP57209_P050) in Human Heart cells using Western Blot
Human THP-1; WB Suggested Anti-RIOK2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: THP-1 cell lysate; RIOK2 antibody - middle region (ARP57210_P050) in Human THP-1 cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIOK2 (RC201484, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIOK2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIOK2 (RC201484, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIOK2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505140)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505140)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505140)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505140)
Anti-RIOK2 mouse monoclonal antibody (TA505140) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIOK2(RC201484).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIOK2 (RC201484, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIOK2.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RIOK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505177)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505177)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505177)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RIOK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505177)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIOK2 (RC201484, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIOK2.
HEK293T cells transfected with either RC201484 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RIOK2 antibody(TA505177), and then analyzed by flow cytometry.
WB Suggested Anti-RIOK2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: THP-1 cell lysate
WB Suggested Anti-RIOK2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human heart
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA505140 | RIOK2 antibody |
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| RIOK2 mouse monoclonal antibody, clone OTI3E11 (formerly 3E11) | |||||||
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Mouse | IgG1 | OTI3E11 | Purified | Hu, Mky, Ms | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody!
|
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| TA505177 | RIOK2 antibody |
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| RIOK2 mouse monoclonal antibody, clone OTI5D11 (formerly 5D11) | |||||||
 |
Mouse | IgG1 | OTI5D11 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA505085 | RIOK2 antibody |
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| RIOK2 mouse monoclonal antibody, clone OTI2F4 (formerly 2F4) | |||||||
 |
Mouse | IgG1 | OTI2F4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA505135 | RIOK2 antibody |
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| RIOK2 mouse monoclonal antibody, clone OTI3F6 (formerly 3F6) | |||||||
 |
Mouse | IgG1 | OTI3F6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP13532PU-N | RIOK2 (C-term) antibody |
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP13533PU-N | RIOK2 (Center) antibody |
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN201484 | RIOK2 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201484BN | RIOK2 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314838 | Riok2 - mouse gene knockout kit via CRISPR |
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|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314838BN | Riok2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314838LP | Riok2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314838RB | Riok2 - mouse gene knockout kit via CRISPR |
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|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760270 | RIOK2 (full length, N-term HIS tag, transcript variant 1) |
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| Recombinant protein of human RIO kinase 2 (yeast) (RIOK2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402668 | RIOK2 overexpression lysate |
|
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |