RIPK2 antibody
Principal name
RIPK2 antibody
Alternative names for RIPK2 antibody
CARDIAK, RICK, RIP2, RIP-2, Receptor-interacting protein 2, Receptor-interacting serine/threonine-protein kinase 2, CARD-containing IL-1 beta ICE-kinase, RIP-like-interacting CLARP kinase
Ncbi ID
9606, NP_003812.1, NP_003812, O43353, NM_003821, AF027706, NP_620402.1, NM_138952
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (detection) (E(detection)), Immunoprecipitation (IP), EPair for Elisa (EPair)
Background of RIPK2 antibody
RIPK2(Receptor-interacting serine/threonine-protein kinase 2), also known as CARD3, CARDIAK, RICK, RIP2, is an enzyme that in humans is encoded by the RIPK2 gene. It has 540-amino acid protein in length. Northern blot analysis revealed that RICK is expressed in various human tissues as 2.5- and 1.8-kb mRNAs that differ due to alternative polyadenylation. RICK is a novel kinase that may regulate apoptosis induced by the FAS receptor pathway. This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-κB and inducer of apoptosis in response to various stimuli, CARDIAK (CARD-containing ICE-associated kinase) specifically interacted with the CARD of ICE/caspase-1, and this interaction correlated with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 subunit.
Western Blot analysis of RIPK2 expression in transfected 293T cell line by RIPK2 monoclonal antibody (M01), clone 2C7.Lane 1: RIPK2 transfected lysate(61.2 KDa).Lane 2: Non-transfected lysate.
RIPK2 monoclonal antibody (M01), clone 2C7. Western Blot analysis of RIPK2 expression in NIH/3T3 ( Cat # L018V1 ).
RIPK2 monoclonal antibody (M01), clone 2C7. Western Blot analysis of RIPK2 expression in Raw 264.7 ( Cat # L024V1 ).
RIPK2 monoclonal antibody (M01), clone 2C7 Western Blot analysis of RIPK2 expression in HeLa ( Cat # L013V1 ).
RIPK2 monoclonal antibody (M01), clone 2C7. Western Blot analysis of RIPK2 expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged RIPK2 is approximately 0.3ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml]
RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in NIH/3T3 ( Cat # L018V1 ).
RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in Raw 264.7 ( Cat # L024V1 ).
RIPK2 monoclonal antibody (M02), clone 6F7 Western Blot analysis of RIPK2 expression in HeLa ( Cat # L013V1 ).
RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml]
RIPK2 monoclonal antibody (M03), clone 3D9. Western Blot analysis of RIPK2 expression in NIH/3T3 ( Cat # L018V1 ).
RIPK2 monoclonal antibody (M03), clone 3D9. Western Blot analysis of RIPK2 expression in Raw 264.7 ( Cat # L024V1 ).
RIPK2 monoclonal antibody (M03), clone 3D9 Western Blot analysis of RIPK2 expression in HeLa ( Cat # L013V1 ).
RIPK2 monoclonal antibody (M03), clone 3D9. Western Blot analysis of RIPK2 expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
RIPK2 monoclonal antibody (M05), clone 7F5 Western Blot analysis of RIPK2 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.
Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunocytochemical staining of A431 cells with Rabbit anti RIP-2 (AP05787PU-N)
Western blot analysis of whole cell lysates from A431 (A) and K562 (K) cells probed with Rabbit anti RIP-2 (AP05787PU-N)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RIPK2 polyclonal antibody ( Cat # PAB3498 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of RIPK2 polyclonal antibody ( Cat # PAB3498 ) in mouse cerebellum tissue lysate. RIPK2 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot analysis of RIPK2 expression in transfected 293T cell line (H00008767-T01) by RIPK2 MaxPab polyclonal antibody.Lane 1: RIPK2 transfected lysate(59.4 KDa).Lane 2: Non-transfected lysate.
(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (RIGHT)Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIPK2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
(LEFT) Western blot analysis of anti-RIPK2 Pab in Ramos cell line lysates (35ug/lane). RIPK2 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-RIPK2 Pab in mouse cerebellum tissue lysate. RIPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot analysis of RIPK2 expression in transfected 293T cell line (H00008767-T02) by RIPK2 MaxPab polyclonal antibody.Lane 1: RIPK2 transfected lysate(61.20 KDa).Lane 2: Non-transfected lysate.
RIPK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of RIPK2 expression in HeLa.
Immunocytochemistry of RIPK2 in A-431 cells with RIPK2 polyclonal antibody ( Cat # PAB13078 ) at 10 µg/ml.
Western blot analysis of RIPK2 in A-431 ( A ) and K-562 ( K ) whole cell lysate with RIPK2 polyclonal antibody ( Cat # PAB13078 ) at 1 : 1000 dilution.
Immunoblot analysis of cell lysates.Lane 1 : HeLa cell lysate. Lnae 2 : 293T cell lysate.Lane 3 : Ramos cell lysate.Lane 4 : C6 ell lysate.Lane 5 : L929 cell lysate.
Detection of endogenous human RIPK2 in different human cell lines by RIPK2 monoclonal antibody, clone Nick-1 ( Cat # MAB3727 ). Total cell extracts ( 50 µg ) from Jurkat ( lane 1 ) , THP-1 ( lane 2 ) and HT-1080 ( lane 3 ).
Rat thyrocytes-FRTL-5; . Sample Type :. Rat thyrocytes-FRTL-5 . Primary Antibody Dilution :. 1:100 . Secondary Antibody :. Anti-rabbit-Texas Red . Secondary Antibody Dilution :. 1:100 . Color/Signal Descriptions :. Red: RIPK2Blue: DAPI. Gene Name :. RIPK2. Submitted by :. Syed A Morshed, Mount Sinai School of Medicine and James J Peters VA Medical Center; RIPK2 antibody - middle region (AVARP02047_P050) in Rat thyrocytes-FRTL-5 cells using Immunofluorescence
Human HepG2; WB Suggested Anti-RIPK2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; RIPK2 antibody - middle region (AVARP02047_P050) in Human HepG2 cells using Western Blot
Western blot analysis of RICK in A431 (A) and K562 (K) whole cell lysate with RICK antibody at 1:1000 dilution.
Immunocytochemistry of RICK in A431 cells with RICK antibody at 10 ug/mL.
Western blot analysis of RICK in A431 cell lysate with RICK antibody at (A) 1 and (B) 2 µg/ml.
Immunocytochemistry of RICK in K562 cells with RICK antibody at 5 µg/ml
Immunofluorescence of RICK in K562 cells with RICK antibody at 20 ug/mL.
Western blot analysis of extracts from rat brain cells, using RIPK2 (Ab-176) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HepG2 cells (Lane 2), using RIPK2 (Ab-176) Antibody. The lane on the left is treated with systhesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using RIPK2 (Ab-176) antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-RIPK2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
Sample Type : Rat thyrocytes-FRTL-5 Primary Antibody Dilution : 1:100 Secondary Antibody : Anti-rabbit-Texas Red Secondary Antibody Dilution : 1:100 Color/Signal Descriptions : Red: RIPK2 Blue: DAPIGene Name : RIPK2 Submitted by : Syed A Morshed, Mount Si
Western blot analysis of extracts of various cell lines, using RIPK2 antibody.
Immunohistochemical of paraffin-embedded human liver cancer using RIPK2 antibody at dilution of 1:100 (200x lens).
Immunohistochemical of paraffin-embedded mouse kidney using RIPK2 antibody at dilution of 1:100 (200x lens).
Immunofluorescence analysis of HeLa cell using RIPK2 antibody. Blue: DAPI for nuclear staining.
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- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332524 | RIPK2 antibody |
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| Rabbit anti-RIPK2 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA305984 | RIPK2 (N-term) antibody |
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| Rabbit Polyclonal RICK Antibody | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA306013 | RIPK2 (C-term) antibody |
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| Rabbit Polyclonal RICK Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA312994 | RIPK2 antibody |
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| Rabbit polyclonal RIPK2 (Ab-176) antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AP05787PU-N | RIPK2 (N-term) antibody |
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Rabbit | IgG | Purified | Hu, Ms, Rt | C, ICC/IF, WB |
0.1 mg /
€410.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP07344PU-N | RIPK2 antibody |
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Rabbit | Aff - Purified | Hu | ICC/IF, P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| AP14642PU-N | RIPK2 (C-term) antibody |
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Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302530 | RIPK2 |
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| Recombinant protein of human receptor-interacting serine-threonine kinase 2 (RIPK2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418408 | RIPK2 overexpression lysate |
|
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208618L1V | Lenti ORF particles, Ripk2 (Myc-DDK-tagged) - Mouse receptor (TNFRSF)-interacting serine-threonine kinase 2 (Ripk2), 200 uL, >10^7 TU/mL |
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200 µl /
€1,110.00
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| OriGene Technologies, Inc. | ||
