RNASEH1 antibody
Principal name
RNASEH1 antibody
Alternative names for RNASEH1 antibody
Ribonuclease H1, RNase H1, Ribonuclease H type II, RNH1
Ncbi ID
9606, NP_002927, AAH02973, NP_002927.2, NM_001013097, NM_002936
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB)
Background of RNASEH1 antibody
- Kit Component:
- - KN200595G1, RNASEH1 gRNA vector 1 in pCas-Guide vector
- KN200595G2, RNASEH1 gRNA vector 2 in pCas-Guide vector
- KN200595D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Nowotny,M., (2008) EMBO J. 27 (7), 1172-1181
Western Blot analysis of RNASEH1 expression in transfected 293T cell line by RNASEH1 monoclonal antibody (M01), clone 5D10.Lane 1: RNASEH1 transfected lysate(32.1 KDa).Lane 2: Non-transfected lysate.
RNASEH1 monoclonal antibody (M01), clone 5D10. Western Blot analysis of RNASEH1 expression in PC-12 ( Cat # L012V1 ).
RNASEH1 monoclonal antibody (M01), clone 5D10 Western Blot analysis of RNASEH1 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged RNASEH1 is approximately 0.03ng/ml as a capture antibody.
Human Jurkat; WB Suggested Anti-RNASEH1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. RNASEH1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; RNASEH1 antibody - middle region (ARP40434_P050) in Human Jurkat cells using Western Blot
WB Suggested Anti-RNASEH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate; RNASEH1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300595 | RNASEH1 |
||||
| Recombinant protein of human ribonuclease H1 (RNASEH1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205003L2V | Lenti ORF particles, Rnaseh1 (GFP-tagged ORF) - Rat ribonuclease H1 (Rnaseh1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR205003L1V | Lenti ORF particles, Rnaseh1 (Myc-DDK-tagged ORF) - Rat ribonuclease H1 (Rnaseh1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||