RNF114 antibody
Principal name
RNF114 antibody
Alternative names for RNF114 antibody
RING finger protein 114, ZNF228, ZNF313, Zinc finger protein 228, Zinc finger protein 313
Ncbi ID
9606, NP_061153, NP_061153.1, NM_018683, NP_109668.2, NM_001001517
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair)
Background of RNF114 antibody
- Kit Component:
- - KN314892G1, Rnf114 gRNA vector 1 in pCas-Guide vector
- KN314892G2, Rnf114 gRNA vector 2 in pCas-Guide vector
- KN314892D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
"Identification of a novel human zinc finger protein gene ZNF313." Ma Y.-X., Zhang S., Hou Y., Huang X., Wu Q., Sun Y. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 35:230-237(2003)
"Identification of ZNF313/RNF114 as a novel psoriasis susceptibility gene." Capon F., Bijlmakers M.-J., Wolf N., Quaranta M., Huffmeier U., Allen M., Timms K., Abkevich V., Gutin A., Smith R., Warren R.B., Young H.S., Worthington J., Burden A.D., Griffiths C.E.M., Hayday A., Nestle F.O., Reis A., Trembath R.C. Hum. Mol. Genet. 17:1938-1945(2008)
Immunoperoxidase of monoclonal antibody to ZNF313 on formalin-fixed paraffin-embedded human lateral ventricle wall. [antibody concentration 3 ug/ml]
Western Blot analysis of ZNF313 expression in transfected 293T cell line by ZNF313 monoclonal antibody (M01), clone 4G3-1A10.Lane 1: ZNF313 transfected lysate(25.7 KDa).Lane 2: Non-transfected lysate.
ZNF313 monoclonal antibody (M01), clone 4G3-1A10 Western Blot analysis of ZNF313 expression in HepG2 ( Cat # L019V1 ).
Detection limit for recombinant GST tagged ZNF313 is approximately 0.3ng/ml as a capture antibody.
Transfected 293T; WB Suggested Anti-ZNF313 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; ZNF313 antibody - N-terminal region (ARP33164_T100) in Transfected 293T cells using Western Blot
Immunofluorescence of purified MaxPab antibody to ZNF313 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of RNF114 expression in transfected 293T cell line (H00055905-T01) by RNF114 MaxPab polyclonal antibody.Lane 1: ZNF313 transfected lysate(25.08 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF114 expression in transfected 293T cell line (H00055905-T02) by RNF114 MaxPab polyclonal antibody.Lane 1: ZNF313 transfected lysate(25.70 KDa).Lane 2: Non-transfected lysate.
Human COLO205; WB Suggested Anti-ZNF313 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate. RNF114 is strongly supported by BioGPS gene expression data to be expressed in Human COLO205 cells.; ZNF313 antibody - C-terminal region (ARP39375_P050) in Human COLO205 cells using Western Blot
Sample (30 ug of whole cell lysate). A:Hep G2 . 12 % SDS PAGE. TA308636 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZNF313 (TA308636) antibody at 1:200 dilution.
WB Suggested Anti-ZNF313 Antibody Titration: 1.25ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T
WB Suggested Anti-ZNF313 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: COLO205 cell lysate; RNF114 is strongly supported by BioGPS gene expression data to be expressed in Human COLO205 cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308636 | RNF114 antibody |
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| Rabbit polyclonal antibody to ZNF313 (ring finger protein 114) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309752 | RNF114 |
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| Recombinant protein of human ring finger protein 114 (RNF114) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412984 | RNF114 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR208566L2V | Lenti ORF particles, Rnf114 (GFP-tagged ORF) - Rat ring finger protein 114 (Rnf114), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR208566L1V | Lenti ORF particles, Rnf114 (Myc-DDK-tagged ORF) - Rat ring finger protein 114 (Rnf114), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||