RNF125 antibody
Principal name
RNF125 antibody
Alternative names for RNF125 antibody
RING finger protein 125, TRAC1, TRAC-1, E3 ubiquitin-protein ligase RNF125, T-cell RING activation protein 1
Ncbi ID
9606, NP_060301.2, NP_060301, NM_017831, NP_080577.1, NM_026301
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of RNF125 antibody
- Kit Component:
- - KN314897G1, Rnf125 gRNA vector 1 in pCas-Guide vector
- KN314897G2, Rnf125 gRNA vector 2 in pCas-Guide vector
- KN314897D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged RNF125 is approximately 0.03ng/ml as a capture antibody.
Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of TRAC-1 in spleen lysate. Lane 1 shows pre-immune sera and lane 2 shows antibody tested on human spleen lysate at 1:1000 dilution.
Immunohistochemical analysis of RNF125 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of RNF125 expression in HEK293T (A); THP1 (B); 129Sv (C) whole cell lysates.
Western blot analysis of extracts from K562 cells and HT-29 cells, using RNF125 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 3T3 cells (Lane 2), using RNF125 Antibody. The lane on the left is treated with synthesized peptide.
WB Suggested Anti-RNF125 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: THP-1 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315551 | RNF125 antibody |
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| Rabbit polyclonal anti-RNF125 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY413505 | RNF125 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR220681L2V | Lenti ORF particles, Rnf125 (GFP-tagged) - Mouse ring finger protein 125 (Rnf125), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR220681L1V | Lenti ORF particles, Rnf125 (Myc-DDK-tagged) - Mouse ring finger protein 125 (Rnf125), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||