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RNF128 antibody

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Principal name

RNF128 antibody

Alternative names for RNF128 antibody

RING finger protein 128, GRAIL, E3 ubiquitin-protein ligase RNF128, Gene related to anergy in lymphocytes protein

SwissProt ID

Q29RU0 (Bovin), Q8AWW4 (Xenla), Q8TEB7 (Human), Q9D304 (Mouse)

Gene ID

79589 (RNF128), 66889 (Rnf128)

Ncbi ID

9606, NP_919445, NP_078815.3, NP_075759.3, NM_023270, NM_194463, NM_024539

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Eq (Equine), Prim (Primate)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of RNF128 antibody

RNF128 is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of RNF128 in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of RNF128 was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype.The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

General readings

Soares,L., (2004) Nat. Immunol. 5 (1), 45-54

ARP43311_T100 - RNF128
Mouse CD4 T; . Lanes: 1: CD4 T cell lysate from WT mice, 2: CD4 T cell lysate from WT mice treated with anti-CD3/CD28, 3: CD4 T cell lysate from WT mice treated with FK506, 4: CD4 T cell lysate from WT mice treated with anti-CD3/CD28 and FK506, 5: CD4 T cell lysate from TRPV1 KO mice, 6: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28, 7: CD4 T cell lysate from TRPV1 KO mice treated with FK506, 8: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28 and FK506.. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:10,000. Gene Name: RNF128. Submitted by: Christine Quinley, UCSD.; RNF128 antibody - C-terminal region (ARP43311_T100) in Mouse CD4 T cells using Western Blot
ARP43311_T100 - RNF128
Human HepG2; WB Suggested Anti-RNF128 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate. RNF128 is supported by BioGPS gene expression data to be expressed in HepG2.; RNF128 antibody - C-terminal region (ARP43311_T100) in Human HepG2 cells using Western Blot
ARP59127_P050 - RNF128
Human COLO205; WB Suggested Anti-RNF128 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate. RNF128 is supported by BioGPS gene expression data to be expressed in COLO205.; RNF128 antibody - C-terminal region (ARP59127_P050) in Human COLO205 cells using Western Blot
TA330464 - RNF128
Lanes: 1: CD4 T cell lysate from WT mice, 2: CD4 T cell lysate from WT mice treated with anti-CD3/CD28, 3: CD4 T cell lysate from WT mice treated with FK506, 4: CD4 T cell lysate from WT mice treated with anti-CD3/CD28 and FK506, 5: CD4 T cell lysate from
TA330464 - RNF128
WB Suggested Anti-RNF128 Antibody Titration: 1.25ug/ml; Positive Control: HepG2 cell lysateRNF128 is supported by BioGPS gene expression data to be expressed in HepG2
TA337013 - RNF128
Western Blot: RNF128 Antibody [TA337013] - Western blot analysis of human GRAIL in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen and 4) rat spleen using TA337013 at 1 ug/ml.
TA337013 - RNF128
Immunohistochemistry-Paraffin: RNF128 Antibody [TA337013] - IHC staining of normal human brain using control (rabbit Ig) at 5 ug/ml.

3 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RNF128 antibody
  Rabbit Polyclonal GRAIL/RNF128 Antibody  
Western Blot: RNF128 Antibody [TA337013] - Western blot analysis of human GRAIL in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen and 4) rat spleen using TA337013 at 1 ug/ml. Rabbit Purified Can, Eq, Hu, Ms, Prim, Rt ICC/IF, P, WB
0.1 mg / €395.00
  OriGene Technologies, Inc.
+1 additional image

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rnf128 (GFP-tagged) - Mouse ring finger protein 128 (Rnf128), 200 uL, >10^7 TU/mL
Lenti ORF particles, Rnf128 (GFP-tagged) - Mouse ring finger protein 128 (Rnf128), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Rnf128 (Myc-DDK-tagged) - Mouse ring finger protein 128 (Rnf128), 200 uL, >10^7 TU/mL
Lenti ORF particles, Rnf128 (Myc-DDK-tagged) - Mouse ring finger protein 128 (Rnf128), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

3 Item(s)

per page

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (1)
Clonality
  1. Polyclonal (1)
Target
  1. RNF128 (1)
Reactivity
  1. Canine (Can) (1)
  2. Equine (Eq) (1)
  3. Human (Hu) (1)
  4. Mouse (Ms) (1)
  5. Primate (Prim) (1)
  6. Rat (Rt) (1)
Applications
  1. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  2. Paraffin Sections (P) (1)
  3. Western blot / Immunoblot (WB) (1)
Presentation
  1. Purified (1)
Host
  1. Rabbit (1)
Images
  1. 1 image (1)
Images: Applications
  1. IHC (1)
  2. Western blot / Immunoblot (WB) (1)
Manufacturer
  1. OriGene Technologies (3)
Service
  • OriGene Technologies GmbH
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  • Germany
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