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RNF139 antibody

Principal name

RNF139 antibody

Alternative names for RNF139 antibody

RNF139, TRC8, RING finger protein 139, Translocation in renal carcinoma on chromosome 8

SwissProt ID

Q5RBT7 (Ponab), Q7TMV1 (Mouse), Q8WU17 (Human)

Gene ID

11236 (RNF139), 75841 (Rnf139)

Ncbi ID

9606, NP_009149, NP_009149.2, NM_175226, NM_007218

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of RNF139 antibody

RNF139 is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

General readings

Gemmill RM, West JD, Boldog F, Tanaka N, Robinson LJ, Smith DI, Li F, Drabkin HA. The hereditary renal cell carcinoma 3;8 translocation fuses FHIT to a patched-related gene, TRC8. Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9572-7. PMID: 9689122

Immunoperoxidase of monoclonal antibody to RNF139 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

H00011236-M01

RNF139 monoclonal antibody (M01), clone 3D10 Western Blot analysis of RNF139 expression in HepG2 ( Cat # L019V1 ).

H00011236-M01

Detection limit for recombinant GST tagged RNF139 is approximately 0.03ng/ml as a capture antibody.

H00011236-M01

RNF139 polyclonal antibody ( Cat # PAB12810 ) ( 0.5µg/ml ) staining of human skeletal muscle lysate ( 35µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.

PAB12810

Human MCF-7; WB Suggested Anti-RNF139 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysate. RNF139 is supported by BioGPS gene expression data to be expressed in MCF7.; RNF139 antibody - N-terminal region (ARP43092_P050) in Human MCF-7 cells using Western Blot

ARP43092_P050

TA305669 (0.5ug/ml) staining of Human Skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA305669

WB Suggested Anti-RNF139 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: MCF7 cell lysateRNF139 is supported by BioGPS gene expression data to be expressed in MCF7

TA329812

Primary Antibodies

Catalog No.	Host	Pres.	React.	Applications
TA305669	RNF139 (C-term) antibody
	Goat Anti-TRC8 Antibody
	Goat	Aff - Purified	Can, Hu	E, P, WB	0.1 mg / €360.00
					OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR209908L2V	Lenti ORF particles, Rnf139 (GFP-tagged) - Mouse ring finger protein 139 (Rnf139), 200 uL, >10^7 TU/mL
		200 µl / €1,420.00
		OriGene Technologies, Inc.

MR209908L1V	Lenti ORF particles, Rnf139 (Myc-DDK-tagged) - Mouse ring finger protein 139 (Rnf139), 200 uL, >10^7 TU/mL
		200 µl / €1,270.00
		OriGene Technologies, Inc.

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