RNF141 antibody
Principal name
RNF141 antibody
Alternative names for RNF141 antibody
ZNF230, RING finger protein 141, Zinc finger protein 230
SwissProt ID
Q8WVD5 (Human)
Gene ID
50862 (RNF141)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection))
Background of RNF141 antibody
- Kit Component:
- - KN201861G1, RNF141 gRNA vector 1 in pCas-Guide vector
- KN201861G2, RNF141 gRNA vector 2 in pCas-Guide vector
- KN201861D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Zhang S, Qiu W, Wu H, Zhang G, Huang M, Xiao C, Yang J, Kamp C, Huang X, Huellen K, Yue Y, Pan A, Lebo R, Milunsky A, Vogt PH.
The shorter zinc finger protein ZNF230 gene message is transcribed in fertile male testes and may be related to human spermatogenesis.
Biochem J. 2001 Nov 1;359(Pt 3):721-7.
PMID: 11672448
Human Brain; RNF141 antibody - middle region (ARP34554_P050) in Human Brain cells using Immunohistochemistry
Human kidney; RNF141 antibody - middle region (ARP34554_P050) in Human kidney cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-RNF141 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; RNF141 antibody - middle region (ARP34554_P050) in Human Jurkat cells using Western Blot
Human Jurkat; WB Suggested Anti-ZNF230 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; ZNF230 antibody - N-terminal region (ARP35795_P050) in Human Jurkat cells using Western Blot
Immunoperoxidase of monoclonal antibody to RNF141 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.3 ug/ml]
Western blot analysis of RNF141 over-expressed 293 cell line, cotransfected with RNF141 Validated Chimera RNAi ( Cat # H00050862-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF141 monoclonal antibody (M01), clone 6D9 (Cat # H00050862-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
RNF141 monoclonal antibody (M01), clone 6D9. Western Blot analysis of RNF141 expression in PC-12 ( Cat # L012V1 ).
Western Blot analysis of RNF141 expression in transfected 293T cell line by RNF141 monoclonal antibody (M01), clone 6D9.Lane 1: RNF141 transfected lysate(25.5 KDa).Lane 2: Non-transfected lysate.
RNF141 monoclonal antibody (M01), clone 6D9 Western Blot analysis of RNF141 expression in A-431 ( Cat # L015V1 ).
RNF141 monoclonal antibody (M01), clone 6D9. Western Blot analysis of RNF141 expression in Raw 264.7 ( Cat # L024V1 ).
Detection limit for recombinant GST tagged RNF141 is approximately 0.03ng/ml as a capture antibody.
Human Jurkat; WB Suggested Anti-RNF141 Antibody. . Titration: 1.25 ug/ml. . Positive Control: Jurkat Whole Cell; RNF141 antibody - middle region (ARP34554_T100) in Human Jurkat cells using Western Blot
Western Blot analysis of RNF141 expression in transfected 293T cell line (H00050862-T01) by RNF141 MaxPab polyclonal antibody.Lane 1: RNF141 transfected lysate(25.3 KDa).Lane 2: Non-transfected lysate.
RNF141 polyclonal antibody ( Cat # PAB6397 ) staining ( 1 µg/ml ) of human testis lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot analysis of RNF141 expression in transfected 293T cell line (H00050862-T02) by RNF141 MaxPab polyclonal antibody.Lane 1: RNF141 transfected lysate(25.3 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF141 expression in transfected 293T cell line (H00050862-T02) by RNF141 MaxPab polyclonal antibody.Lane 1: RNF141 transfected lysate(25.3 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF141 expression in transfected 293T cell line (H00050862-T03) by RNF141 MaxPab polyclonal antibody.Lane 1: RNF141 transfected lysate(25.50 KDa).Lane 2: Non-transfected lysate.
TA302869 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
WB Suggested Anti-RNF141 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
Human Brain
Human kidney
WB Suggested Anti-RNF141 Antibody; Titration: 1.25 ug/ml; Positive Control: Jurkat Whole Cell
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302869 | RNF141 (C-term) antibody |
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| Goat Polyclonal Antibody against RNF141 | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301861 | RNF141 |
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| Recombinant protein of human ring finger protein 141 (RNF141) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||