RNF144B antibody
Principal name
RNF144B antibody
Alternative names for RNF144B antibody
RING finger protein 144B, IBRDC2, P53RFP, E3 ubiquitin-protein ligase RNF144B, IBR domain-containing protein 2, p53-inducible RING finger protein
Ncbi ID
9606, NP_877434, NP_877434.2, NM_182757, NP_001164114.1, NM_001170643
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F)
Background of RNF144B antibody
- Kit Component:
- - KN209302G1, RNF144B gRNA vector 1 in pCas-Guide vector
- KN209302G2, RNF144B gRNA vector 2 in pCas-Guide vector
- KN209302D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to IBRDC2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.2 ug/ml]
Immunofluorescence of monoclonal antibody to IBRDC2 on HeLa cell. [antibody concentration 10 ug/ml]
IBRDC2 monoclonal antibody (M02), clone 4F1 Western Blot analysis of IBRDC2 expression in IMR-32 ( Cat # L008V1 ).
Western Blot analysis of RNF144B expression in transfected 293T cell line (H00255488-T01) by RNF144B MaxPab polyclonal antibody.Lane 1: IBRDC2 transfected lysate(33.22 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF144B expression in transfected 293T cell line (H00255488-T02) by RNF144B MaxPab polyclonal antibody.Lane 1: RNF144B transfected lysate(33.60 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF144B expression in transfected 293T cell line (H00255488-T02) by RNF144B MaxPab polyclonal antibody.Lane 1: RNF144B transfected lysate(33.60 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of RNF144B transfected lysate using anti-RNF144B MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RNF144B MaxPab mouse polyclonal antibody (B01) (H00255488-B01).
Human ACHN; WB Suggested Anti-RNF144B Antibody Titration: 0.2-1 ug/ml. Positive Control: ACHN cell lysate; RNF144B antibody - middle region (ARP43479_P050) in Human ACHN cells using Western Blot
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to IBRDC2 on HeLa cell (antibody concentration 10 µg/ml).
RNF144B monoclonal antibody, clone 4F1 Western Blot analysis of IBRDC2 expression in IMR-32.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500696).
Anti-RNF144B mouse monoclonal antibody (TA500696) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500696),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500696), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500696),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunofluorescent staining of HepG2 cells using anti-RNF144B mouse monoclonal antibody (TA500698).
Anti-RNF144B mouse monoclonal antibody (TA500698) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500698), and then analyzed by flow cytometry.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500699).
Anti-RNF144B mouse monoclonal antibody (TA500699) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500699),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500699), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500699),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500704)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500704)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500704)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500704)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500704)
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500704).
Anti-RNF144B mouse monoclonal antibody (TA500704) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500704),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500704),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500704), and then analyzed by flow cytometry.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500705).
Anti-RNF144B mouse monoclonal antibody (TA500705) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500705),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500705), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500708).
Anti-RNF144B mouse monoclonal antibody (TA500708) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500708),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500708), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500708),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-RNF144B mouse monoclonal antibody (TA500709) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500709), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500709),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500710).
Anti-RNF144B mouse monoclonal antibody (TA500710) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500710),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500710), and then analyzed by flow cytometry.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500711).
Anti-RNF144B mouse monoclonal antibody (TA500711) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500711), and then analyzed by flow cytometry.
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500712), and then analyzed by flow cytometry.
Anti-RNF144B mouse monoclonal antibody (TA500722) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RNF144B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500722),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500722),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500722), and then analyzed by flow cytometry.
Western blot analysis of extracts from HeLa cells, using RNF144B antibody.The lane on the right is treated with the synthesized peptide.
- Quick-Filters:
- Highly Validated (8)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500696 | RNF144B antibody |
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| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI3C2 (formerly 3C2) | |||||||
 |
Mouse | IgG1 | OTI3c2 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
|
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| TA500699 | RNF144B antibody |
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| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI11F4 (formerly 11F4) | |||||||
 |
Mouse | IgG1 | OTI11F4 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA500704 | RNF144B antibody |
||||||
| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI9H10 (formerly 9H10) | |||||||
 |
Mouse | IgG2b | OTI9H10 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
|
||||||
| TA500705 | RNF144B antibody |
||||||
| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI6B5 (formerly 6B5) | |||||||
 |
Mouse | IgG1 | OTI6B5 | Purified | Hu | F, ICC/IF |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA500708 | RNF144B antibody |
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| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI3D7 (formerly 3D7) | |||||||
 |
Mouse | IgG1 | OTI3D7 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA500710 | RNF144B antibody |
||||||
| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI11G1 (formerly 11G1) | |||||||
 |
Mouse | IgG1 | OTI11G1 | Purified | Hu | F, ICC/IF |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
||||||
| TA500722 | RNF144B antibody |
||||||
| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI6B11 (formerly 6B11) | |||||||
 |
Mouse | IgG1 | OTI6B11 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| AM21023PU-N | RNF144B (1-101) antibody |
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 |
Mouse | IgG2a | 4F1 | Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +4 additional images |
Highly validated antibody! |
||||||
| TA312549 | RNF144B antibody |
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| Rabbit polyclonal anti-RNF144B antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA500698 | RNF144B antibody |
||||||
| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI7H2 (formerly 7H2) | |||||||
 |
Mouse | IgG1 | OTI7H2 | Purified | Hu | F, ICC/IF |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA500706 | RNF144B antibody |
||||||
| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI10A12 (formerly 10A12) | |||||||
 |
Mouse | IgG1 | OTI10A12 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA500709 | RNF144B antibody |
||||||
| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI12B6 (formerly 12B6) | |||||||
 |
Mouse | IgG1 | OTI12B6 | Purified | Hu | F, ICC/IF |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA500711 | RNF144B antibody |
||||||
| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI1G5 (formerly 1G5) | |||||||
 |
Mouse | IgG1 | OTI1G5 | Purified | Hu | F, ICC/IF |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA500712 | RNF144B antibody |
||||||
| RNF144B (IBRDC2) mouse monoclonal antibody, clone OTI1E1 (formerly 1E1) | |||||||
 |
Mouse | IgG1 | OTI1E1 | Purified | Hu | F |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Lysates
| Catalog No. | ||
|---|---|---|
| LY405351 | RNF144B overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201133L2V | Lenti ORF particles, Rnf144b (GFP-tagged) - Mouse ring finger protein 144B (Rnf144b), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€840.00
|
|
| OriGene Technologies, Inc. | ||
| MR201133L1V | Lenti ORF particles, Rnf144b (Myc-DDK-tagged) - Mouse ring finger protein 144B (Rnf144b), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€740.00
|
|
| OriGene Technologies, Inc. | ||