RNF2 antibody
Principal name
RNF2 antibody
Alternative names for RNF2 antibody
RING finger protein 2, BAP1, BAP-1, DING, HIPI3, RING1B, E3 ubiquitin-protein ligase RING2, HIP2-interacting protein 3, Protein DinG
Ncbi ID
NP_009143, NP_009143.1, 9606, NM_007212, NP_035407.1, NM_011277, NM_001025667
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)
Background of RNF2 antibody
- Kit Component:
- - KN314934G1, Rnf2 gRNA vector 1 in pCas-Guide vector
- KN314934G2, Rnf2 gRNA vector 2 in pCas-Guide vector
- KN314934D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Voncken,J.W., et al., (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (5), 2468-2473
Human Daudi; WB Suggested Anti-RNF2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Daudi cell lysate. RNF2 is supported by BioGPS gene expression data to be expressed in Daudi.; RNF2 antibody - middle region (ARP34289_P050) in Human Daudi cells using Western Blot
R1502P RNF2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 2.5 µg/ml followed by biotinylated anti-Goat IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
Western blot using Affinity Purified anti-RING1B shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temperature. Signal was detected using standard techniques.
Western blot analysis of RNF2 over-expressed 293 cell line, cotransfected with RNF2 Validated Chimera RNAi ( Cat # H00006045-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF2 monoclonal antibody (M01), clone 6C2 (Cat # H00006045-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
RNF2 monoclonal antibody (M01), clone 6C2. Western Blot analysis of RNF2 expression in Raw 264.7 ( Cat # L024V1 ).
Western Blot analysis of RNF2 expression in transfected 293T cell line by RNF2 monoclonal antibody (M01), clone 6C2.Lane 1: RNF2 transfected lysate(37.7 KDa).Lane 2: Non-transfected lysate.
RNF2 monoclonal antibody (M01), clone 6C2 Western Blot analysis of RNF2 expression in NIH/3T3 ( Cat # L018V1 ).
Detection limit for recombinant GST tagged RNF2 is approximately 0.03ng/ml as a capture antibody.
Human kidney; RNF2 antibody - middle region (ARP34290_T100) in Human kidney cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-RNF2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; RNF2 antibody - middle region (ARP34290_T100) in Human HepG2 cells using Western Blot
RNF2 polyclonal antibody ( Cat # PAB6331 ) staining ( 1 µg/ml ) of human bone marrow lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in PC-12(Cat # L012V1 ).
RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in human stomach.
RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in NIH/3T3(Cat # L018V1 ).
RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in Hela NE ( Cat # L013V3 ).
RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in Raw 264.7(Cat # L024V1 ).
Detection limit for recombinant GST tagged RNF2 is approximately 1ng/ml as a capture antibody.
RNF2 monoclonal antibody (M05), clone 2B6. Western Blot analysis of RNF2 expression in human placenta.
RNF2 monoclonal antibody (M05), clone 2B6. Western Blot analysis of RNF2 expression in NIH/3T3(Cat # L018V1 ).
RNF2 monoclonal antibody (M05), clone 2B6. Western Blot analysis of RNF2 expression in Raw 264.7(Cat # L024V1 ).
RNF2 monoclonal antibody (M05), clone 2B6. Western Blot analysis of RNF2 expression in PC-12(Cat # L012V1 ).
RNF2 monoclonal antibody (M05), clone 2B6. Western Blot analysis of RNF2 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged RNF2 is approximately 1ng/ml as a capture antibody.
RNF2 monoclonal antibody (M06), clone 4A9. Western Blot analysis of RNF2 expression in human placenta.
RNF2 monoclonal antibody (M06), clone 4A9. Western Blot analysis of RNF2 expression in NIH/3T3(Cat # L018V1 ).
RNF2 monoclonal antibody (M06), clone 4A9. Western Blot analysis of RNF2 expression in Raw 264.7(Cat # L024V1 ).
RNF2 monoclonal antibody (M06), clone 4A9. Western Blot analysis of RNF2 expression in PC-12(Cat # L012V1 ).
RNF2 monoclonal antibody (M06), clone 4A9. Western Blot analysis of RNF2 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged RNF2 is approximately 3ng/ml as a capture antibody.
RNF2 monoclonal antibody (M08), clone 3B8. Western Blot analysis of RNF2 expression in human placenta.
RNF2 monoclonal antibody (M08), clone 3B8. Western Blot analysis of RNF2 expression in NIH/3T3(Cat # L018V1 ).
RNF2 monoclonal antibody (M08), clone 3B8. Western Blot analysis of RNF2 expression in Raw 264.7(Cat # L024V1 ).
RNF2 monoclonal antibody (M08), clone 3B8. Western Blot analysis of RNF2 expression in PC-12(Cat # L012V1 ).
RNF2 monoclonal antibody (M08), clone 3B8. Western Blot analysis of RNF2 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged RNF2 is approximately 3ng/ml as a capture antibody.
RNF2 monoclonal antibody (M09), clone 4A12. Western Blot analysis of RNF2 expression in HepG2 ( Cat # L019V1 ).
RNF2 monoclonal antibody (M09), clone 4A12. Western Blot analysis of RNF2 expression in HeLa ( Cat # L013V1 ).
RNF2 monoclonal antibody (M12), clone 4C1. Western Blot analysis of RNF2 expression in human stomach.
RNF2 monoclonal antibody (M12), clone 4C1. Western Blot analysis of RNF2 expression in human liver.
RNF2 monoclonal antibody (M13), clone 2F7. Western Blot analysis of RNF2 expression in HepG2(Cat # L019V1 ).
RNF2 monoclonal antibody (M14), clone 2B4. Western Blot analysis of RNF2 expression in NIH/3T3(Cat # L018V1 ).
RNF2 monoclonal antibody (M14), clone 2B4. Western Blot analysis of RNF2 expression in Raw 264.7(Cat # L024V1 ).
RNF2 monoclonal antibody (M14), clone 2B4. Western Blot analysis of RNF2 expression in A-549 ( Cat # L025V1 ).
RNF2 monoclonal antibody (M14), clone 2B4. Western Blot analysis of RNF2 expression in PC-12(Cat # L012V1 ).
RNF2 monoclonal antibody (M14), clone 2B4. Western Blot analysis of RNF2 expression in HepG2 ( Cat # L019V1 ).
Detection limit for recombinant GST tagged RNF2 is approximately 3ng/ml as a capture antibody.
Western blot using RNF2 polyclonal antibody ( Cat # PAB10232 ) shows detection of a 38 kDa band corresponding to human RNF2 in 3T3 ( lane 1 ), U937 ( lane 2 ), Jurkat ( lane 3 ), mouse brain ( lane 4 ) and CHO-K1 ( lane 5 ) cell lysates.Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilutionof RNF2 polyclonal antibody incubated at room temperature.Signal was detected using standard techniques.
Western blot analysis of Ring1B expression in several cells using AM26485AF-N.
Immunoprecipitation of Ring1B from U937 cells with AM26485AF-N (1) or normal mouse IgG (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26485AF-N.
TA302592 staining (0.5 µg/ml) of K562 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of extracts of various cell lines, using RNF2 antibody.
Immunohistochemistry of paraffin-embedded human breast cancer using RNF2 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of U20S cell using RNF2 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-RNF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Daudi cell lysate; RNF2 is supported by BioGPS gene expression data to be expressed in Daudi
WB Suggested Anti-RNF2 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
Human kidney
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- Cited (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302592 | RNF2 (C-term) antibody |
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| Goat Polyclonal Antibody against RNF2 / dinG | |||||||
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Goat | Aff - Purified | Bov, Can, Hu, Ms | E, P, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA332768 | RNF2 antibody |
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| Rabbit anti-RNF2 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AM26485AF-N | RNF2 antibody |
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Mouse | IgG2b | 3-3 | Azide Free | Hst, Hu, Ms | IP, WB |
0.1 mg /
€480.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| R1502P | RNF2 (189-201) antibody |
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Goat | Aff - Purified | Bov, Can, Chimp, Chk, Frog, Hu, Ms, Orang-Utan, Rt, Ze | E, ICC/IF, P, WB |
0.1 mg /
€515.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303089 | RNF2 |
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| Recombinant protein of human ring finger protein 2 (RNF2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Published recently! |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY402109 | RNF2 overexpression lysate |
|
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR207712L2V | Lenti ORF particles, Rnf2 (GFP-tagged ORF) - Rat ring finger protein 2 (Rnf2), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| RR207712L1V | Lenti ORF particles, Rnf2 (Myc-DDK-tagged ORF) - Rat ring finger protein 2 (Rnf2), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
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| OriGene Technologies, Inc. | ||
