RNF31 antibody
Principal name
RNF31 antibody
Alternative names for RNF31 antibody
RING finger protein 31, HOIL-1-interacting protein, HOIP, ZIBRA, Zinc in-between-RING-finger ubiquitin-associated domain protein
Ncbi ID
9606, NP_060469, NP_919327.2, AAH12077.1, NP_060469.4, NM_194346, NM_017999
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB)
Background of RNF31 antibody
- Kit Component:
- - KN314948G1, Rnf31 gRNA vector 1 in pCas-Guide vector
- KN314948G2, Rnf31 gRNA vector 2 in pCas-Guide vector
- KN314948D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kirisako,T., (2006) EMBO J. 25 (20), 4877-4887
RNF31 polyclonal antibody ( Cat # PAB6229 ) staining ( 2 µg/ml ) of HepG2 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Human A549; . Lanes: Lane1: 50 ug hormoxia A549 lysate. Lane2: 50 ug hypoxia A549 lysate. Lane3: 50 ug hormoxia A549 lysate (+scrambled siRNA). Lane4: 50 ug hypoxia A549 lysate (+scrambled siRNA). Lane5: 50 ug hormoxia A549 lysate (RNF31 siRNA). Lane6: 50 ug hypoxia A549 lysate (RNF31 siRNA). Primary Antibody Dilution: 1:800. Secondary Antibody: Goat anti rabbit HRP . Secondary Antibody Dilution: 1:10000. Gene Name: RNF31. Submitted by: Markus Queisser, Northwestern University.; RNF31 antibody - middle region (ARP43241_P050) in Human A549 cells using Western Blot
Human Jurkat; WB Suggested Anti-RNF31 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; RNF31 antibody - middle region (ARP43241_P050) in Human Jurkat cells using Western Blot
TA302821 staining (2µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
WB Suggested Anti-RNF31 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
Lanes: Lane1: 50 ug hormoxia A549 lysate; Lane2: 50 ug hypoxia A549 lysate; Lane3: 50 ug hormoxia A549 lysate (+scrambled siRNA); Lane4: 50 ug hypoxia A549 lysate (+scrambled siRNA); Lane5: 50 ug hormoxia A549 lysate (RNF31 siRNA); Lane6: 50 ug hypoxia A5
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302821 | RNF31 (C-term) antibody |
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| Goat Polyclonal Antibody against RNF31 | |||||||
 |
Goat | Aff - Purified | Can, Hu, Por | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN314948 | Rnf31 - mouse gene knockout kit via CRISPR |
||
 |
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314948LP | Rnf31 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314948RB | Rnf31 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211571L2V | Lenti ORF particles, Rnf31 (GFP-tagged) - Mouse ring finger protein 31 (Rnf31), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,980.00
|
|
| OriGene Technologies, Inc. | ||
| MR211571L1V | Lenti ORF particles, Rnf31 (Myc-DDK-tagged) - Mouse ring finger protein 31 (Rnf31), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,780.00
|
|
| OriGene Technologies, Inc. | ||