RNF8 antibody
Principal name
RNF8 antibody
Alternative names for RNF8 antibody
RING finger protein 8, E3 ubiquitin-protein ligase RNF8
Ncbi ID
9606, NP_003949, NM_003958, NP_003949.1, NM_001025727, NM_021419, NM_183078
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of RNF8 antibody
- Kit Component:
- - KN201402G1, RNF8 gRNA vector 1 in pCas-Guide vector
- KN201402G2, RNF8 gRNA vector 2 in pCas-Guide vector
- KN201402D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Seki N, Hattori A, Sugano S, Suzuki Y, Nakagawara A, Ohhira M, Muramatsu M, Hori T, Saito T.
Isolation, tissue expression, and chromosomal assignment of a novel human gene which encodes a protein with RING finger motif.
J Hum Genet. 1998;43(4):272-4.
PMID: 9852682
RNF8 polyclonal antibody ( Cat # PAB6337 ) staining ( 0.1 µg/ml ) of human lung lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunofluorescence of purified MaxPab antibody to RNF8 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of RNF8 expression in transfected 293T cell line (H00009025-T01) by RNF8 MaxPab polyclonal antibody.Lane 1: RNF8 transfected lysate(53.35 KDa).Lane 2: Non-transfected lysate.
Human Heart; RNF8 antibody - C-terminal region (ARP40071_T100) in Human Heart cells using Immunohistochemistry
Immunofluorescence of purified MaxPab antibody to RNF8 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of RNF8 expression in transfected 293T cell line (H00009025-T02) by RNF8 MaxPab polyclonal antibody.Lane 1: RNF8 transfected lysate(53.35 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RNF8 expression in transfected 293T cell line (H00009025-T02) by RNF8 MaxPab polyclonal antibody.Lane 1: RNF8 transfected lysate(55.50 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of RNF8 Antibody (Cat. #AP53695PU-N) in Jurkat cell line lysates (35µg/lane). RNF8 (arrow) was detected using the purified Pab.
RNF8 Antibody (Cat. #AP53695PU-N) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA302855 staining (0.1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of RNF8 in human lung tissue lysate with RNF8 antibody at (A) and (B) 2 ug/mL.
Immunofluorescence of RNF8 in rat lung tissue with RNF8 antibody at 20 ug/mL.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302855 | RNF8 (N-term) antibody |
||||||
| Goat Polyclonal Antibody against RNF8 | |||||||
 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA319765 | RNF8 (C-term) antibody |
||||||
| Rabbit Polyclonal RNF8 Antibody | |||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€415.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| AP53695PU-N | RNF8 antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418324 | RNF8 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207821L2V | Lenti ORF particles, Rnf8 (GFP-tagged) - Mouse ring finger protein 8 (Rnf8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204268L2V | Lenti ORF particles, Rnf8 (GFP-tagged ORF) - Rat ring finger protein 8 (Rnf8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,170.00
|
|
| OriGene Technologies, Inc. | ||
| RR204268L1V | Lenti ORF particles, Rnf8 (Myc-DDK-tagged ORF) - Rat ring finger protein 8 (Rnf8), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,050.00
|
|
| OriGene Technologies, Inc. | ||