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ROBO1 antibody

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Principal name

ROBO1 antibody

Alternative names for ROBO1 antibody

ROBO-1, Roundabout homolog 1, DUTT1, Deleted in U Twenty Twenty

SwissProt ID

O55005 (Rat), O89026 (Mouse), Q9Y6N7 (Human)

Gene ID

58946 (Robo1), 6091 (ROBO1), 19876

Ncbi ID

NP_071524, 9606, NP_002932.1, NP_062286.2, NP_002932, NM_019413, NM_133631, NM_002941, NM_001145844

Available reactivities

Rt (Rat), Hu (Human), Ms (Mouse), Bt (Bat), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Fi (Fish), Hst (Hamster), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Xen (Xenopus), Tr (Turkey), L (Lizard), Bear

Available hosts

Goat, Mouse, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ROBO1 antibody

ROBO-1 (also called Roundabout homolog 1 precursor and Deleted in U twenty twenty (DUTT)) functions as a receptor for SLIT1 and SLIT2. The SLIT proteins are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. ROBO-1 may also be required for lung development. ROBO-1 is a type I membrane protein. ROBO-1 is a widely expressed protein with the exception of the kidney. Defects in ROBO1 may be a cause of breast and lung cancer. ROBO-1 maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line. Multiple splice variants have been identified for this protein.

General readings

1. Andrews W, Liapi A, Plachez C, Camurri L, Zhang J, Mori S, Murakami F, Parnavelas JG, Sundaresan V, Richards LJ.
Robo1 regulates the development of major axon tracts and interneuron migration in the forebrain. Development. 2006 Jun;133(11):2243-52. PMID: 16690755

H00006091-M01 - ROBO1
Detection limit for recombinant GST tagged ROBO1 is approximately 0.03ng/ml as a capture antibody.
AP05287PU-N - ROBO1
Western blot using ROBO1 antibody (AP05287PU-N) on HT-29 cell lysate. Lysate used at 30 µg/lane. Antibody used at 1:400 dilution. Secondary antibody, mouse anti-rabbit HRP, used at 1:50k dilution.
AP05287SU-N - ROBO1
Western blot using ROBO1 antibody (AP05287SU-N) on human brain lysate. Lysate used at 15 µg/lane. Antibody used at 10 µg/ml. Secondary antibody, mouse anti rabbit HRP, used at 1:50k.
AP07917PU-N - ROBO1
Figure 1. Staining ROBO1 in Brain by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
PAB6930 - ROBO1
ROBO1 polyclonal antibody ( Cat # PAB6930 ) ( 1 µg/ml ) staining of HUVEC lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
H00006091-M03 - ROBO1
Detection limit for recombinant GST tagged ROBO1 is approximately 0.1ng/ml as a capture antibody.
PAB10401 - ROBO1
Western blot using ROBO1 polyclonal antibody ( Cat # PAB10401 ) on human brain lysate. Lysate used at 15 µg/lane. Antibody used at 10 µg/ml.
PAB11258 - ROBO1
Immunohistochemistry of ROBO1 polyclonal antibody ( Cat # PAB11258 ) was used at a concentration of 5 µg/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows staining of human brain tissue.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
PAB11258 - ROBO1
Western blot using ROBO1 polyclonal antibody ( Cat # PAB11258 ) shows detection of a band at ~181 kDa correspond-ing to ROBO1 present in mouse brain lysate ( arrowhead ).Approximately 35 µg of lysate was separated by 4-8% SDS-PAGE and probed with the primary antibody diluted to 1:1,000.
AP22501PU-N - ROBO1
Human Liver (formalin-fixed, paraffin-embedded) stained with ROBO1 antibody AP22501PU-N at 2 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AP22501PU-N - ROBO1
Antibody (1 µg/ml) staining of HUVEC lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
AP23652PU-N - ROBO1
AP23652PU-N DUTT1 antibody staining of fetal Mouse Brain lysate at 0.05 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA303077 - ROBO1
TA303077 (1µg/ml) staining of HUVEC lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA319298 - ROBO1
WB using Anti-ROBO-1 antibody shows detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 µg of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
TA319298 - ROBO1
Affinity Purified anti-ROBO1 antibody was used at a concentration of 5 µg/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
TA319298 - ROBO1
1/50 staining mouse lung tissue sections (adult, frozen 100µm wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C.
TA319298 - ROBO1
Staining of ROBO1 in undifferentiated, immortalized human podocytes by ICC/ IF. Cells were fixed with 2% paraformaldehyde and 4% sucrose at RT for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at 1/200.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ROBO1 (1632-1644) antibody
  Rabbit polyclonal anti-ROBO-1 antibody  
WB using Anti-ROBO-1 antibody shows detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 µg of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Rabbit IgG Aff - Purified Can, Hu, Ms, Rt E, P, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

ROBO1 antibody
AP23652PU-N DUTT1 antibody staining of fetal Mouse Brain lysate at 0.05 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Ms, Rt E, WB
0.1 mg / €380.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Western blotting.

ROBO1 antibody
Western blot using ROBO1 antibody (AP05287PU-N) on HT-29 cell lysate. Lysate used at 30 µg/lane. Antibody used at 1:400 dilution. Secondary antibody, mouse anti-rabbit HRP, used at 1:50k dilution. Rabbit Aff - Purified Hu WB
0.1 mg / €400.00
  OriGene Technologies GmbH

ROBO1 antibody
Western blot using ROBO1 antibody (AP05287SU-N) on human brain lysate. Lysate used at 15 µg/lane. Antibody used at 10 µg/ml. Secondary antibody, mouse anti rabbit HRP, used at 1:50k. Rabbit Purified Hu WB
0.1 mg / €400.00
  OriGene Technologies GmbH

ROBO1 (1632-1644) antibody
Figure 1. Staining ROBO1 in Brain by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Can, Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

ROBO1 (994-1006) antibody
Human Liver (formalin-fixed, paraffin-embedded) stained with ROBO1 antibody AP22501PU-N at 2 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Rt, Xen E, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+1 additional image

ROBO1 antibody
ROBO-1 staining of cryostat tissue sections of rat embryo (Neural Tube) at  a concentration of 5 µg/mL using HRP-DAB detection (brown) and counterstained with hematoxylin (blue). Goat Aff - Purified Rt C, E, WB
0.1 mg / €450.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

ROBO1 - human gene knockout kit via CRISPR
ROBO1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ROBO1 - human gene knockout kit via CRISPR
ROBO1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ROBO1 - human gene knockout kit via CRISPR
ROBO1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ROBO1 - human gene knockout kit via CRISPR
ROBO1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ROBO1 (mGFP-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, ROBO1 (mGFP-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €2,680.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, ROBO1 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, ROBO1 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €2,420.00
  OriGene Technologies, Inc.
 

13 Item(s)

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13 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (4)
  2. Human Lenti ORF Particle (2)
  3. Primary Antibody (7)
Clonality
  1. Polyclonal (7)
Target
  1. ROBO1 (7)
Property
  1. 1632-1644 (2)
  2. 994-1006 (1)
Reactivity
  1. Human (Hu) (5)
  2. Rat (Rt) (5)
  3. Canine (Can) (4)
  4. Mouse (Ms) (4)
  5. Bovine (Bov) (1)
  6. Bat (Bt) (1)
  7. Chicken (Chk) (1)
  8. Elephant (1)
  9. Equine (Eq) (1)
  10. Hamster (Hst) (1)
  11. Monkey (Mky) (1)
  12. Porcine (Por) (1)
  13. Xenopus (Xen) (1)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Enzyme Immunoassay (E) (5)
  3. Paraffin Sections (P) (3)
  4. Frozen sections (C) (1)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Aff - Purified (6)
  2. Purified (1)
Host
  1. Rabbit (4)
  2. Goat (3)
Images
  1. 1 image (7)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (2)
  3. Immunofluorescence (IF) (1)
  4. IHC (1)
Species (CRISPR)
  1. Human (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (12)
  2. Neuromics Antibodies (1)
Service
  • OriGene Technologies GmbH
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  • free call: 0800-227 47 46 (Germany only)
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