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ROCK1 antibody

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Principal name

ROCK1 antibody

Alternative names for ROCK1 antibody

ROCK-1, p160ROCK, NY-REN-35, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1

SwissProt ID

O77819 (Rabit), P70335 (Mouse), Q13464 (Human), Q63644 (Rat), Q8MIT6 (Bovin)

Gene ID

6093 (ROCK1), 19877 (Rock1)

Ncbi ID

NP_005397.1, 9606, NP_005397, NM_005406, NP_033097.1, NM_009071

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Por (Porcine), Rb (Rabbit), Can (Canine), Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of ROCK1 antibody

The Rho target protein, Rho-associated coiled coil-containing protein kise (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-termil kise domain and an inhibitory cysteine/histidine-rich C-termil domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-termil inhibitory domain is removed, resulting in deregulated and constitutive kise activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

H00006093-M01 - ROCK1
Immunofluorescence of monoclonal antibody to ROCK1 on HeLa cell. [antibody concentration 10 ug/ml]
H00006093-M01 - ROCK1
ROCK1 monoclonal antibody (M01), clone 2E2 Western Blot analysis of ROCK1 expression in HeLa NE ( Cat # L013V3 ).
H00006093-M01 - ROCK1
Detection limit for recombinant GST tagged ROCK1 is approximately 0.03ng/ml as a capture antibody.
AP20652PU-N - ROCK1
Immunohistochemistry analyzes of Rock-1 antibody (Cat.-No.: AP20652PU-N) in paraffin-embedded human brain tissue.
AP20652PU-N - ROCK1
Western blot (WB) analysis of Rock-1 antibody (Cat.-No.: AP20652PU-N) in extracts from COS7 cells.
AP26128CP-N - ROCK1
Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
CPA2013-100ul - ROCK1
Immunofluorescent analysis of ROCK1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA2013-100ul - ROCK1
Western blot analysis of ROCK1 expression in HeLa (A); NIH3T3 (B); C6 (C); COS7 (D) whole cell lysates.
TA308951 - ROCK1
ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis. A. 30 ug NIH-3T3 whole cell extract. B. 30 ug C2Cl2 whole cell extract. 5 % SDS-PAGE. ROCK1 antibody [N1N2], N-term (TA308951) dilution: 1:1000
TA308951 - ROCK1
ROCK1 antibody [N1N2], N-term detects ROCK1 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 5 % SDS-PAGE. ROCK1 antibody [N1N2], N-term (TA308951) dilution: 1:500
TA308951 - ROCK1
Sample (30 ug of whole cell lysate). A: A431 . B: H1299. C: Molt-4 . 5% SDS PAGE. TA308951 diluted at 1:1000.
TA308951 - ROCK1
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft , using ROCK1(TA308951) antibody at 1:500 dilution.
TA308951 - ROCK1
Immunofluorescence analysis of methanol-fixed HeLa, using ROCK1(TA308951) antibody at 1:200 dilution.
TA308951 - ROCK1
ROCK1 antibody immunoprecipitates ROCK1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of ROCK1 by 3 ug of ROCK1 antibody (TA308951) 5% SDS-PAGE The immunoprecipi
TA319700 - ROCK1
Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
TA319700 - ROCK1
Immunofluorescence of ROCK1 in 293 cells with ROCK1 antibody at 20 ug/mL.
TA319700 - ROCK1
Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 ug/mL.
TA322615 - ROCK1
Predicted cell location: Cytoplasm. Positive control: Human breast and gastric cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ROCK1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200)
TA322615 - ROCK1
Predicted cell location: Cytoplasm. Positive control: Human breast and gastric cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using ROCK1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
TA322616 - ROCK1
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ROCK1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
TA325814 - ROCK1
Western blot analysis of ROCK1 expression in Hela whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA336423 - ROCK1
Western Blot: cleaved ROCK1 Antibody (154C1465) [TA336423] - Analysis of cleaved ROCK-1 in A) untreated, B) 1 hour and C) 4 hour staurosporin-treated (2 uM) Jurkat cells using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL subst
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11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ROCK1 antibody
  Rabbit polyclonal antibody to ROCK1 (Rho-associated, coiled-coil containing protein kinase 1)  
ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis. A. 30 ug NIH-3T3 whole cell extract. B. 30 ug C2Cl2 whole cell extract. 5 % SDS-PAGE. ROCK1 antibody [N1N2], N-term (TA308951) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+5 additional images

Highly validated antibody!

ROCK1 (N-term) antibody
  Rabbit Polyclonal ROCK1 Antibody  
Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

ROCK1 antibody
  Anti-ROCK1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human breast and gastric cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ROCK1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

ROCK1 antibody
  Anti-ROCK1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ROCK1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

ROCK1 antibody
  Rabbit Polyclonal ROCK1 Antibody  
Western blot analysis of ROCK1 expression in Hela whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

ROCK1 antibody
  Mouse Monoclonal cleaved ROCK1 Antibody (154C1465)  
Western Blot: cleaved ROCK1 Antibody (154C1465) [TA336423] - Analysis of cleaved ROCK-1 in A) untreated, B) 1 hour and C) 4 hour staurosporin-treated (2 uM) Jurkat cells using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL subst Mouse IgG 154C1465 Purified Can, Hu, Ms, Prim, Rb, Rt WB
0.1 mg / €415.00
  OriGene Technologies, Inc.

ROCK1 antibody
ROCK1 Mouse IgG 12M03 Purified Hu WB
0.1 mg / €415.00
  OriGene Technologies GmbH

ROCK1 antibody
Immunohistochemistry analyzes of Rock-1 antibody (Cat.-No.: AP20652PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

Lysates

Catalog No.    

ROCK1 overexpression lysate
ROCK1 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ROCK1 (mGFP-tagged) - Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), 200 uL, >10^7 TU/mL
Lenti ORF particles, ROCK1 (mGFP-tagged) - Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), 200 uL, >10^7 TU/mL  
200 µl / €2,680.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, ROCK1 (Myc-DDK tagged) - Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1) , 200 uL, >10^7 TU/mL
Lenti ORF particles, ROCK1 (Myc-DDK tagged) - Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1) , 200 uL, >10^7 TU/mL  
200 µl / €2,420.00
  OriGene Technologies, Inc.
 

11 Item(s)

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11 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Human Lenti ORF Particle (2)
  2. Lysate (1)
  3. Primary Antibody (8)
Clonality
  1. Polyclonal (6)
  2. Monoclonal (2)
Target
  1. ROCK1 (8)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (8)
  2. Rat (Rt) (7)
  3. Mouse (Ms) (6)
  4. Canine (Can) (1)
  5. Primate (Prim) (1)
  6. Rabbit (Rb) (1)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (4)
  3. Enzyme Immunoassay (E) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Purified (5)
  2. Aff - Purified (3)
Host
  1. Rabbit (6)
  2. Mouse (2)
Images
  1. 1 image (7)
  2. 3+ images (2)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. IHC (3)
  3. Immunofluorescence (IF) (2)
  4. Immunoprecipitation (IP) (1)
  5. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (11)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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