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RPA1 antibody

Principal name

RPA1 antibody

Alternative names for RPA1 antibody

REPA1, RFA1, RF-A protein 1, RPA70, RP-A p70, Replication protein A, Replication protein A 70 kDa DNA-binding subunit, Replication factor A protein 1, Single-stranded DNA-binding protein

SwissProt ID

P22336 (Yeast), P27694 (Human), Q01588 (Xenla), Q19537 (Caeel), Q24492 (Drome), Q5ZJJ2 (Chick), Q6NY74 (Danre), Q8VEE4 (Mouse), Q92372 (Schpo)

Gene ID

6117 (RPA1), 851266 (RFA1), 68275 (Rpa1)

Ncbi ID

9606, NP_009404, NP_002936, NP_002936.1, NM_002945, NP_001157695.1, NM_026653, NM_001164223

Available reactivities

Hu (Human), S. cerevisiae (Saccharomyces cerevisiae), Ye (Yeast), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ChIP assay (ChIP), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)

Background of RPA1 antibody

RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded D binding protein, whose D binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular D metabolism such as D replication, recombition, cell cycle and D damage checkpoints, and all major types of D repair including nucleotide excision, base excision, mismatch and double-strand break repairs. In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 checkpoint complex. RPA is hyperphosphorylated upon D damage or replication stress by checkpoint kises including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and D-dependent protein kise (D-PK). Hyperphosphorylation may alter RPA-D and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PC and Pol a, and also proteins involved in SV40 replication, such as D polymerase I and SV40 large T antigen .

General readings

1. Mol Cell. 2009 Oct 23;36(2):193-206.
J Biol Chem. 2009 Dec 11;284(50):34682-91.

RPA1 polyclonal antibody (A01), Lot # 050727JC01 Western Blot analysis of RPA1 expression in Y-79 ( Cat # L042V1 ).

H00006117-A01

TCA precipitated protein extracts from a wild type yeast strain ( S. cerevisiae ) were separated on 10% gel and transferred to a PVDF membrane.RFA1/RFA2/RFA3 polyclonal antibody ( Cat # PAB13584 ) was used in different dilutions : 1 : 5,000 ( Lane 1 ), 1 : 10,000 ( Lane 2 ) and 1 : 20,000 ( Lane 3 ).Besides the bands for RFA1 and RFA2 an unspecific band was detected at ~150 kDa.

PAB13584

Human HeLa; WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Hela cell lysate. RPA1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; RPA1 antibody - middle region (ARP56525_P050) in Human HeLa cells using Western Blot

ARP56525_P050

Human 721_B; Host:Rabbit. Target Name:RPA1. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. RPA1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; RPA1 antibody - middle region (ARP56526_P050) in Human 721_B cells using Western Blot

ARP56526_P050

Human Hela; Host:Rabbit. Target Name:RPA1. Sample Tissue:Human Hela. Antibody Dilution: 1.0ug/ml. RPA1 is strongly supported by BioGPS gene expression data to be expressed in HeLa.; RPA1 antibody - middle region (ARP56526_P050) in Human Hela cells using Western Blot

ARP56526_P050

Human MCF7; Host:Rabbit. Target Name:RPA1. Sample Tissue:Human MCF7. Antibody Dilution: 1.0ug/ml. RPA1 is supported by BioGPS gene expression data to be expressed in MCF7.; RPA1 antibody - middle region (ARP56526_P050) in Human MCF7 cells using Western Blot

ARP56526_P050

Human HepG2; WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; RPA1 antibody - middle region (ARP56526_P050) in Human HepG2 cells using Western Blot

ARP56526_P050

Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using RPA1 antibody Cat.-No AM06744PU-N with DAB staining.

AM06744PU-N

Immunofluorescence analysis of HeLa cells using RPA1 antibody Cat.-No AM06744PU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

AM06744PU-N

Left: Western blot analysis using RPA1 mAb against human RPA1 (AA: 308-513) recombinant protein (Expected MW is 48.3 kDa). Right: Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562(3), A431(4), and COS-7 (6) cell lysate.

AM06744PU-N

AM06744PU-N

Flow Cytometric analysis of Jurkat cells using RPA1 antibody Cat.-No AM06744PU-N (green) and negative control (red).

AM06744PU-N

Western blot analysis of RPA1 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.

TP302066

Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308716 diluted at 1:1000.

TA308716

Sample(30 ug whole cell lysate). A:H1299. B:HeLa S3. 7.5% SDS PAGE. TA308716 diluted at 1:1000

TA308716

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit (TA308716) antibody at 1:100 dilution.

TA308716

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit (TA308716) antibody at 1:200 dilution.

TA308716

Anti-RPA1 (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA320208

Predicted band size: 68 kDa . Positive control: A431 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA321545

WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysateRPA1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells

TA342197

WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate

TA342198

Host:Rabbit; Target Name:RPA1; Sample Tissue:Human Hela; Antibody Dilution: 1.0 ug/mlRPA1 is strongly supported by BioGPS gene expression data to be expressed in HeLa

TA342198

Host:Rabbit; Target Name:RPA1; Sample Tissue:Human 721_B; Antibody Dilution: 1.0 ug/mlRPA1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

TA342198

Host:Rabbit; Target Name:RPA1; Sample Tissue:Human MCF7; Antibody Dilution: 1.0 ug/mlRPA1 is supported by BioGPS gene expression data to be expressed in MCF7

TA342198

Western blot detection of RPA70 in Hela,A431,MCF7,COS7,HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

TA347042

Western blot detection of RPA70 in Hela,293T,C6,3T3 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

TA347042

Western blot detection of RPA70 in HUVEC,Hacat,3T3,MCF7 and A549 cell lysates using RPA70 mouse mAb (1:2000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

TA347042

Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA70 mouse mAb (dilution 1:100).

TA347042

Immunoprecipitation analysis of Hela cell lysates using RPA70 mouse mAb.

TA347042

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA308716	RPA1 antibody
	Rabbit Polyclonal antibody to RPA70 (replication protein A1, 70kDa)
	Rabbit	IgG		Aff - Purified	Hu, Ms	ICC/IF, P, WB	0.1 ml / €415.00
							OriGene Technologies, Inc.
+3 additional images	Highly validated antibody!
TA347042	RPA1 antibody
	Mouse Monoclonal RPA70 Antibody
	Mouse	IgG2a	8C3-D12-H10	Purified		ICC/IF, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+4 additional images	Highly validated antibody!
AM06744PU-N	RPA1 antibody
	Mouse	IgG1	4C4	Purified	Hu, Mky	E, F, ICC/IF, P, WB	0.1 mg / €410.00
							OriGene Technologies GmbH
+4 additional images	Highly validated antibody!
TA320208	RPA1 (323-337) antibody
	Goat Polyclonal RPA1/RPA70 Antibody
	Goat			Aff - Purified	Hu	E, WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA321545	RPA1 antibody
	Anti-RPA1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP302066	RPA1
	Recombinant protein of human replication protein A1, 70kDa (RPA1)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY401030	RPA1 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR209516L2V	Lenti ORF particles, Rpa1 (GFP-tagged) - Mouse replication protein A1 (Rpa1), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €1,360.00
		OriGene Technologies, Inc.

MR209516L1V	Lenti ORF particles, Rpa1 (Myc-DDK-tagged) - Mouse replication protein A1 (Rpa1), transcript variant 2, 200 uL, >10^7 TU/mL
		200 µl / €1,220.00
		OriGene Technologies, Inc.

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