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RPL18 antibody

Principal name

RPL18 antibody

Alternative names for RPL18 antibody

60S ribosomal protein L18

SwissProt ID

P35980 (Mouse), Q07020 (Human), Q5E973 (Bovin), Q95342 (Pig), Q9VS34 (Drome)

Gene ID

6141 (RPL18), 19899 (Rpl18)

Ncbi ID

9606, NP_000970, NP_000970.1, NP_033103.2, BC081468, NM_009077, NM_000979

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of RPL18 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 R species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 R species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

Western Blot analysis of RPL18 expression in transfected 293T cell line (H00006141-T02) by RPL18 MaxPab polyclonal antibody.Lane 1: RPL18 transfected lysate(21.60 KDa).Lane 2: Non-transfected lysate.

H00006141-D01P

RPL18 MaxPab rabbit polyclonal antibody. Western Blot analysis of RPL18 expression in HepG2.

H00006141-D01P

Human Adult Liver; Rabbit Anti-RPL18 Antibody . Catalog Number: ARP56127_P050. Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver . Observed Staining: Cytoplasm in hepatocytes, very strong signal, low tissue distribution. Primary Antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20X. Exposure Time: 0.5 â€“ 2.0 sec. Protocol located in Reviews and Data.; RPL18 antibody - N-terminal region (ARP56127_P050) in Human Adult Liver cells using Immunohistochemistry

ARP56127_P050

Human HT1080; WB Suggested Anti-RPL18 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HT1080 cell lysate. RPL18 is supported by BioGPS gene expression data to be expressed in HT1080.; RPL18 antibody - N-terminal region (ARP56127_P050) in Human HT1080 cells using Western Blot

ARP56127_P050

Western blot analysis of extracts from HeLa/COLO cells, using RPL18 Antibody. The lane on the right is treated with the synthesized peptide.

Formalin-Fixed, Paraffin-Embedded Human Testis stained with RPL18 Antibody Cat.-No AP33222PU-N

AP33222PU-N

Western blot analysis of extracts from HeLa/COLO cells, using RPL18 Antibody Cat.-No AP33222PU-N. The lane on the right is treated with the synthesized peptide.

AP33222PU-N

Immunohistochemical analysis of RPL18 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of RPL18 expression in Jurkat (A); HeLa (B); NIH3T3 (C) whole cell lysates.

TP306317

Western blot analysis of extracts from COLO cells and HeLa cells, using RPL18 antibody.The lane on the right is treated with the synthesized peptide.

TA314462

WB Suggested Anti-RPL18 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HT1080 cell lysateRPL18 is supported by BioGPS gene expression data to be expressed in HT1080

TA335159

Rabbit Anti-RPL18 Antibody; Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver; Observed Staining: Cytoplasm in hepatocytes, very strong signal, low tissue distribution; Primary Antibody Concentration: 1:100; Secondary Antibody: Donkey anti-Rabbit

TA335159

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP306317	RPL18
	Recombinant protein of human ribosomal protein L18 (RPL18)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

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