RPL35 antibody
Principal name
RPL35 antibody
Alternative names for RPL35 antibody
60S ribosomal protein L35
SwissProt ID
P17078 (Rat), P34662 (Caeel), P39741 (Yeast), P42766 (Human), Q29361 (Pig), Q3MHM7 (Bovin), Q6ZWV7 (Mouse), Q8JHJ1 (Danre), Q98TF7 (Chick)
Ncbi ID
9606, NP_009140.1, NP_009140, NM_007209, NP_079868.1, NM_025592, NM_212511
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)
Background of RPL35 antibody
- Kit Component:
- - KN315040G1, Rpl35 gRNA vector 1 in pCas-Guide vector
- KN315040G2, Rpl35 gRNA vector 2 in pCas-Guide vector
- KN315040D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Odintsova T.I., et al. (2003) J. Protein Chem. 22:249-258Choudhary C., et al. (2009) Science. 325:834-840
Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human pancreas tissue using RPL35 Antibody (C-term) Cat.-No. AP53717PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RPL35 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis in WiDr cell line lysates (35 ug/lane) using RPL35 Antibody (C-term) Cat.-No. AP53717PU-N. This demonstrates the RPL35 antibody detected the RPL35 protein (arrow).
Western blot analysis in NIH-3T3 cell line lysates (35 ug/lane) using RPL35 Antibody (C-term) Cat.-No. AP53717PU-N. This demonstrates the RPL35 antibody detected the RPL35 protein (arrow).
Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex stained with RPL35 Antibody Cat.-No AP33221PU-N.
Western blot analysis of extracts from 293/HeLa/COLO/Jurkat cells, using RPL35 Antibody Cat.-No AP33221PU-N. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of RPL35 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of RPL35 expression in U2OS (A); HeLa (B); NIH3T3 (C) whole cell lysates.
Western blot analysis of extracts from HeLa cells, Jurkat cells, COLO cells and 293 cells, using RPL35 antibody.The lane on the right is treated with the synthesized peptide.
Host: Rabbit; Target Name: Rpl35; Sample Tissue: Mouse Small Intestine lysates; Antibody Dilution: 1.0 ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315163 | RPL35 antibody |
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| Rabbit polyclonal anti-RPL35 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP33221PU-N | RPL35 (51-100) antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
| AP53717PU-N | RPL35 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303088 | RPL35 |
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| Recombinant protein of human ribosomal protein L35 (RPL35) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416124 | RPL35 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR219837L2V | Lenti ORF particles, Rpl35 (GFP-tagged) - Mouse ribosomal protein L35 (Rpl35), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR219837L1V | Lenti ORF particles, Rpl35 (Myc-DDK-tagged) - Mouse ribosomal protein L35 (Rpl35), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204964L2V | Lenti ORF particles, Rpl35 (GFP-tagged ORF) - Rat ribosomal protein L35 (Rpl35), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR204964L1V | Lenti ORF particles, Rpl35 (Myc-DDK-tagged ORF) - Rat ribosomal protein L35 (Rpl35), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||