RPL36 antibody
Principal name
RPL36 antibody
Alternative names for RPL36 antibody
60S ribosomal protein L36
SwissProt ID
P39032 (Rat), P47964 (Mouse), P49181 (Caeel), P49630 (Drome), P52864 (Mycpn), Q9Y3U8 (Human)
Ncbi ID
9606, NP_056229.2, NP_378669, NP_378669.1, NM_015414, NP_056229, NM_022504, NM_033643
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB)
Background of RPL36 antibody
- Kit Component:
- - KN200054G1, RPL36 gRNA vector 1 in pCas-Guide vector
- KN200054G2, RPL36 gRNA vector 2 in pCas-Guide vector
- KN200054D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis in A2058, MDA-MB435, 293 cell line lysates (35 ug/lane) using RPL36 Antibody (N-term) Cat.-No. AP53718PU-N. RPL36 (arrow) was detected using the purified Pab.
Immunohistochemical analysis of RPL36 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of RPL36 staining in MDAMB435 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of RPL36 expression in MDAMB435 (A); HEK293 (B); Raw264.7 (C); mouse brain (D); rat brain (E) whole cell lysates.
Western blot analysis of extracts from COLO cells, using RPL36 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using RPL36 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using RPL36 antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315292 | RPL36 antibody |
||||||
| Rabbit polyclonal anti-RPL36 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| AP53718PU-N | RPL36 (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300054 | RPL36 (transcript variant 2) |
||||
| Recombinant protein of human ribosomal protein L36 (RPL36), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP760740 | RPL36 (full length, N-term HIS tag, transcript variant 1) |
||||
| Purified recombinant protein of Human ribosomal protein L36 (RPL36), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414576 | RPL36 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR207260L2V | Lenti ORF particles, Rpl36 (GFP-tagged ORF) - Rat ribosomal protein L36 (Rpl36), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR207260L1V | Lenti ORF particles, Rpl36 (Myc-DDK-tagged ORF) - Rat ribosomal protein L36 (Rpl36), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
