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RSPO1 antibody

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Principal name

RSPO1 antibody

Alternative names for RSPO1 antibody

R-spondin-1, Roof plate-specific spondin-1

SwissProt ID

Q2MKA7 (Human), Q6DHR0 (Danre), Q9Z132 (Mouse)

Gene ID

284654 (RSPO1), 192199 (Rspo1)

Ncbi ID

NP_001033722.1, NP_001033722, NM_001038633, NP_619624.2, NM_138683, NM_001242909

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB)

Background of RSPO1 antibody

R-spondin1, also known as RSPO1, CRISTIN3, FLJ40906. Entrez Protein NP_001033722.1. It is a member of the R-spondin family and a 28.9kDa secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestil epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.It is activator of the beta-catenin sigling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor sigling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determition.

AP23479PU-N - RSPO1
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP23479PU-N - RSPO1
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP23479PU-N - RSPO1
Western blot analysis of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 µg/ml.
AM06311SU-N - RSPO1
Western blot analysis using R-spondin1 mouse mAb against recombinant R-spondin1 protein (Lane 1) and R-spondin1(aa21-263)-hIgGFc transfected HEK293 cell lysate (Lane 2).
TA590755 - RSPO1
ELISA: RSPO1 Antibody
TA804459 - RSPO1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RSPO1 (RC217921, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RSPO1.
TA804531 - RSPO1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RSPO1 (RC217921, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RSPO1.
TA804626 - RSPO1
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RSPO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804626)
TA804626 - RSPO1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RSPO1 (RC217921, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RSPO1.
TA804766 - RSPO1
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RSPO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804766)
TA804766 - RSPO1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RSPO1 (RC217921, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RSPO1.
TA306782 - RSPO1
Western blot analysis of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 ug/ml.
TA306782 - RSPO1
Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 2.5 ug/ml.
TA306782 - RSPO1
Immunofluorescence of RSPO1 in Human Spleen tissue with RSPO1 antibody at 20 ug/mL.

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RSPO1 antibody
  Rabbit Polyclonal RSPO1 Antibody  
Western blot analysis of RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

RSPO1 antibody
  Purified RSPO1 mouse monoclonal antibody, clone OTI2B11 (formerly 2B11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RSPO1 (RC217921, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RSPO1. Mouse IgG2a OTI2B11 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

RSPO1 antibody
  Purified RSPO1 mouse monoclonal antibody, clone OTI2F10 (formerly 2F10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RSPO1 (RC217921, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RSPO1. Mouse IgG1 OTI2F10 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

RSPO1 antibody
  RSPO1 mouse monoclonal antibody, clone OTI11A9 (formerly 11A9)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RSPO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804626) Mouse IgG2b OTI11A9 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

RSPO1 antibody
  RSPO1 mouse monoclonal antibody, clone OTI1C3 (formerly 1C3)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RSPO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804766) Mouse IgG1 OTI1C3 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

RSPO1 antibody
Western blot analysis using R-spondin1 mouse mAb against recombinant R-spondin1 protein (Lane 1) and R-spondin1(aa21-263)-hIgGFc transfected HEK293 cell lysate (Lane 2). Mouse IgG1 7A6 Ascites Hu WB
0.1 ml / €380.00
  OriGene Technologies GmbH

RSPO1 (N-term) antibody
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu, Ms, Rt E, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+2 additional images

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RSPO1
  Purified recombinant protein of Human R-spondin homolog (Xenopus laevis) (RSPO1).  
RSPO1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.		 CHO cells
20 µg / €230.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

RSPO1 overexpression lysate
RSPO1 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

9 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (7)
  3. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (5)
  2. Polyclonal (2)
Target
  1. RSPO1 (8)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Paraffin Sections (P) (4)
  3. Enzyme Immunoassay (E) (2)
Presentation
  1. Purified (4)
  2. Aff - Purified (1)
  3. Ascites (1)
Host
  1. Mouse (5)
  2. Rabbit (2)
Images
  1. 1 image (7)
  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Paraffin Sections (P) (3)
  3. Immunofluorescence (IF) (1)
  4. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. CHO cells (1)
Manufacturer
  1. OriGene Technologies (9)
Service
  • OriGene Technologies GmbH
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