RTN4IP1 antibody
Principal name
RTN4IP1 antibody
Alternative names for RTN4IP1 antibody
NIMP, Reticulon-4-interacting protein 1 mitochondrial, NOGO-interacting mitochondrial protein
Ncbi ID
9606, NM_032730, NP_116119, NP_116119.2, NP_570962.2, NM_130892
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of RTN4IP1 antibody
- Kit Component:
- - KN315192G1, Rtn4ip1 gRNA vector 1 in pCas-Guide vector
- KN315192G2, Rtn4ip1 gRNA vector 2 in pCas-Guide vector
- KN315192D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Huber A.B. et al. (2000) Biol. Chem. 381: 407-419.Hu W.H. et al. (2002) J. Neurochem. 81: 36-45.
Western Blot analysis of RTN4IP1 expression in transfected 293T cell line (H00084816-T01) by RTN4IP1 MaxPab polyclonal antibody.Lane 1: RTN4IP1 transfected lysate(43.56 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RTN4IP1 expression in transfected 293T cell line (H00084816-T02) by RTN4IP1 MaxPab polyclonal antibody.Lane 1: RTN4IP1 transfected lysate(43.56 KDa).Lane 2: Non-transfected lysate.
Human Fetal Kidney; WB Suggested Anti-RTN4IP1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Kidney; RTN4IP1 antibody - middle region (ARP60655_P050) in Human Fetal Kidney cells using Western Blot
Immunohistochemistry analysis in Formalin Fixed and Paraffin Embedded Human hepatocarcinoma using RT4I1 Antibody (C-term) Cat.-No AP53761PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RT4I1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis in WiDr cell line lysates (35 µg/lane) using RT4I1 Antibody (C-term) Cat.-No AP53761PU-N. This demonstrates the RT4I1 antibody detected the RT4I1 protein (arrow).
Flow Cytometry analysis of WiDr cells using RT4I1 Antibody (C-term) Cat.-No AP53761PU-N (Right histogram) compared to a negative control cell (Left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RTN4IP1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTN4IP1 (RC202957, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1.
HEK293T cells transfected with either RC202957 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RTN4IP1 antibody(TA502543), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RTN4IP1 antibody(TA502543),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-RTN4IP1 antibody(TA502543),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502544)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502544)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502544)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502544)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502544)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502544)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502544)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTN4IP1 (RC202957, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1.
HEK293T cells transfected with either RC202957 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RTN4IP1 antibody(TA502544), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-RTN4IP1 antibody(TA502544),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RTN4IP1 antibody(TA502544),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RTN4IP1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTN4IP1 (RC202957, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1.
HEK293T cells transfected with either RC202957 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RTN4IP1 antibody(TA502545), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RTN4IP1 antibody(TA502545),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-RTN4IP1 antibody(TA502545),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTN4IP1 (RC202957, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1.
Flow cytometric Analysis of Hela cells, using anti-RTN4IP1 antibody(TA502623),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RTN4IP1 antibody(TA502623),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504093)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RTN4IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504093)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTN4IP1 (RC202957, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1.
WB Suggested Anti-RTN4IP1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Kidney
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502543 | RTN4IP1 antibody |
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| RTN4IP1 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2) | |||||||
 |
Mouse | IgG1 | OTI1B2 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
||||||
| TA502544 | RTN4IP1 antibody |
||||||
| RTN4IP1 mouse monoclonal antibody, clone OTI3B2 (formerly 3B2) | |||||||
 |
Mouse | IgG1 | OTI3B2 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
|
||||||
| TA502545 | RTN4IP1 antibody |
||||||
| RTN4IP1 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1) | |||||||
 |
Mouse | IgG1 | OTI1E1 | Purified | Hu | F, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA502623 | RTN4IP1 antibody |
||||||
| RTN4IP1 mouse monoclonal antibody, clone OTI2A9 (formerly 2A9) | |||||||
 |
Mouse | IgG1 | OTI2A9 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA504093 | RTN4IP1 antibody |
||||||
| RTN4IP1 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8) | |||||||
 |
Mouse | IgG2b | OTI1A8 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP53761PU-N | RTN4IP1 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN202957 | RTN4IP1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN202957BN | RTN4IP1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN202957LP | RTN4IP1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN202957RB | RTN4IP1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302957 | RTN4IP1 |
||||
| Recombinant protein of human reticulon 4 interacting protein 1 (RTN4IP1), nuclear gene encoding mitochondrial protein | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY409966 | RTN4IP1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC202957L1V | Lenti ORF particles, RTN4IP1 (Myc-DDK tagged) - Human reticulon 4 interacting protein 1 (RTN4IP1), nuclear gene encoding mitochondrial protein , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,000.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206202L2V | Lenti ORF particles, Rtn4ip1 (GFP-tagged) - Mouse reticulon 4 interacting protein 1 (Rtn4ip1), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR206202L1V | Lenti ORF particles, Rtn4ip1 (Myc-DDK-tagged) - Mouse reticulon 4 interacting protein 1 (Rtn4ip1), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
