RYBP antibody
Principal name
RYBP antibody
Alternative names for RYBP antibody
RING1 and YY1-binding protein, DEDAF, YEAF1, AAP-1, APAP-1, DED-associated factor, YY1 and E4TF1-associated factor 1, Death effector domain-associated factor, Apoptin-associating protein 1
Ncbi ID
NP_036366.3, 9606, NP_036366, NM_012234, AF179286, NP_062717.2, NM_019743
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Flow Cytometry (F)
Background of RYBP antibody
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor . DEDAF is identical to the transcriptional repressor RYBP (2). DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
General readings
Chen D, et al. (2008) EMBO Rep. 10(2):166-72. Zheng L., et al. (2001) J. Biol. Chem. 276:31945-31952
Western blot analysis of DEDAF expression in HepG2 cell lysate.
Western Blot analysis of RYBP expression in transfected 293T cell line (H00023429-T02) by RYBP MaxPab polyclonal antibody.Lane 1: RYBP transfected lysate(25.08 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to RYBP on HeLa cell. [antibody concentration 10 ug/ml]
RYBP MaxPab polyclonal antibody. Western Blot analysis of RYBP expression in human liver.
Western Blot analysis of RYBP expression in transfected 293T cell line (H00023429-T01) by RYBP MaxPab polyclonal antibody.Lane 1: RYBP transfected lysate(25.19 KDa).Lane 2: Non-transfected lysate.
Figure 2. Formalin-Fixed Paraffin-Embedded (FFPE) on Spleen.
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Placenta.
Immunofluorescence of purified MaxPab antibody to RYBP on HeLa cell. [antibody concentration 10 ug/ml]
RYBP MaxPab polyclonal antibody. Western Blot analysis of RYBP expression in human liver.
Western Blot analysis of RYBP expression in transfected 293T cell line (H00023429-T01) by RYBP MaxPab polyclonal antibody.Lane 1: RYBP transfected lysate(25.19 KDa).Lane 2: Non-transfected lysate.
Human Muscle; WB Suggested Anti-RYBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle; RYBP antibody - C-terminal region (ARP38973_P050) in Human Muscle cells using Western Blot
Human, Mouse; . Lanes: 1. 50 ug 38B9 cell lysate, 2. 50 ug CH12 cell lysate, 3. 50 ug HeLa cell lysate. 4. Purified RYBP Protein. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-Rabbit HRP. Secondary Antibody Dilution: 1:30000. Gene Name: RYBP. Submitted by: Dr. Arimdam Basu, Dept. of Animal Biology, University of Pennsylvania.; RYBP antibody - C-terminal region (ARP38973_P050) in Human, Mouse cells using Western Blot
Western Blot analysis of RYBP expression in transfected 293T cell line (H00023429-T01) by RYBP MaxPab polyclonal antibody.Lane 1: RYBP transfected lysate(25.19 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry of RYBP in mouse liver tissue with RYBP polyclonal antibody ( Cat # PAB12843 ) at 10 µg/ml.
Western blot analysis of RYBP expression in human A-549 ( lane A ), HepG2 ( lane B ), and mouse NIH/3T3 ( lane C ) cell lysates with RYBP polyclonal antibody ( Cat # PAB12843 ) at 1 µg /ml.
Western blot analysis of RYBP expression in HepG2 cell lysate.
Immunoprecipitation of RYBP transfected lysate using anti-RYBP MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RYBP MaxPab mouse polyclonal antibody (B02) (H00023429-B02).
Immunoprecipitation of RYBP transfected lysate using anti-RYBP MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RYBP MaxPab mouse polyclonal antibody (B01) (H00023429-B01).
Human Placenta; Placenta; RYBP antibody - middle region (ARP31819_P050) in Human Placenta cells using Immunohistochemistry
Human, Mouse; . Lanes: 1. 50 ug 38B9 cell lysate, 2. 50 ug CH12 cell lysate, 3. 50 ug HeLa cell lysate. 4. Purified RYBP Protein. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-Rabbit HRP. Secondary Antibody Dilution: 1:30000. Gene Name: RYBP. Submitted by: Dr. Arimdam Basu, Dept. of Animal Biology, University of Pennsylvania.; RYBP antibody - middle region (ARP31819_P050) in Human, Mouse cells using Western Blot
Human THP1; WB Suggested Anti-RYBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; RYBP antibody - middle region (ARP31819_P050) in Human THP1 cells using Western Blot
Human Thyroid; . IHC Information: Paraffin embedded thyroid tissue, tested with an antibody Dilution of 5 ug/ml.; RYBP antibody - N-terminal region (ARP38972_P050) in Human Thyroid cells using Immunohistochemistry
Human THP1; WB Suggested Anti-RYBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: THP-1 cell lysate; RYBP antibody - N-terminal region (ARP38972_P050) in Human THP1 cells using Western Blot
Western blot analysis in A375 cell line lysates (35 ug/lane) using RYBP Antibody (N-term) Cat.-No. AP53775PU-N. This demonstrates the RYBP antibody detected the RYBP protein (arrow).
Western blot analysis of RYBP (arrow) using RYBP Antibody (N-term) Cat.-No AP53775PU-N. 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RYBP gene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RYBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504650)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RYBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504650)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RYBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504650)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RYBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504650)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RYBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504650)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RYBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504650)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RYBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504650)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RYBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504650)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RYBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504650)
Anti-RYBP mouse monoclonal antibody (TA504650) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RYBP(RC206186).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RYBP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP.
Anti-RYBP mouse monoclonal antibody (TA504761) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RYBP(RC206186).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP.
HEK293T cells transfected with either RC206186 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RYBP antibody(TA800034), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP.
HEK293T cells transfected with either RC206186 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RYBP antibody(TA800035), and then analyzed by flow cytometry.
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 ug /ml.
Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 10 ug/ml.
Immunofluorescence of DEDAF in A549 cells with DEDAF antibody at 20 ug/mL.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306024 | RYBP antibody |
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| Rabbit Polyclonal DEDAF Antibody | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AP00095PU-N | RYBP antibody |
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 |
Rabbit | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
| AP07729PU-N | RYBP (215-228) antibody |
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 |
Rabbit | Aff - Purified | Bov, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Platypus, Por, Rb, Rt | P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP53775PU-N | RYBP (N-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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