S100A1 antibody
Principal name
S100A1 antibody
Alternative names for S100A1 antibody
S100-A1, S100A, S100 alpha, S100 calcium-binding protein A1, S-100 protein subunit alpha, S-100 protein alpha chain
SwissProt ID
P02639 (Bovin), P23297 (Human), P35467 (Rat), P56565 (Mouse), Q5RC36 (Ponab), Q7LZT1 (Misfo)
Ncbi ID
NP_006262, NP_001092512, 9606, NP_006262.1, NP_001092512.1, NP_035439, NM_006271, NM_011309, NM_001007636
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (capture) (E(capture)), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP)
Background of S100A1 antibody
S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21kDa. In human brain tissue S-100 protein is mainly presented as two isoforms - betabeta homodimer (S-100b) and alphabeta heterodimer (S-100a). Because of its predominant location in astroglial cells S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into the extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 protein in patient serum samples are useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, and in diagnosis and prognosis of clinical outcome in acute stroke. Although predominant among the water-soluble brain proteins, S-100 is also found in a variety of other tissues. S-100 is an intracellular protein that weakly binds calcium. It binds zinc very tightly, however, and this appears to increase the affinity of the protein for calcium. Distinct binding sites, with different affinities, exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Figure 1. SP2091P: S100 antibody staining of Formalin Fixed, Paraffin processed Human melanoma tissue.
Western Blot analysis of S100A1 expression in transfected 293T cell line by S100A1 monoclonal antibody (M01), clone 1D5.Lane 1: S100A1 transfected lysate(10.5 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged S100A1 is approximately 0.3ng/ml as a capture antibody.
Immunohistochemistry analysis of S100A1 antibody (Cat.-No.: AP06310PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of S100 α antibody (Cat.-No.: AP06310PU-N) at 1/500 dilution
Lane1:HEK293T whole cell lysate
Lane2:Mouse brain tissue lysate
Lane3:Rat brain tissue lysate
Lane1:HEK293T whole cell lysate
Lane2:Mouse brain tissue lysate
Lane3:Rat brain tissue lysate
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Prostate, Nerve.
Human melanoma stained with anti-S100 antibody
Formalin-fixed and paraffin-embedded human neurofibroma tissue ( A; 4 µm ) , skin melanoma tissue ( B; 4 µm ) , and human breast tissue ( C; 4 µm ) stained with S100A1 monoclonal antibody, clone D28-E ( Cat # MAB1659 ) show: A - positive immunostaining of tumor cells and peripheral nerve; B - strong positive immunostaining of melanoma cells; C - strong positive immunostaining of the outer myoepithelial cell component in mammary lobule. Kindly performed and provided by Katarina Poliakova, MD and Lubomir Straka, MD, Ph. D., from Clinical Pathology Presov, Ltd., Presov, Slovakia.
Human melanoma stained with S100A1 monoclonal antibody, clone SPM354 ( Cat # MAB2546 ) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Immunohistochemistry of S100A1/S100B polyclonal antibody ( Cat # PAB11341 ) was used at a 1:500 dilution to detect S100A1/S100B by immunohistochemistry in human brain astrocyte tumor tissue.Tissue was formalin-fixed and paraffin embedded.Personal Communication,Alan Yen, Life Span Biosciences, Seattle, WA.
Western blot using S100A1/S100B polyclonal antibody ( Cat # PAB11341 ) shows detection of a band ~11 kDa corresponding to bovine S100A1/S100B monomer ( 100 ng loaded, arrowhead lane 1 ).The antibody also detects S100A1/S100B from rat brain lysate ( lane 2 ). Approximately 35 µg of a rat brain whole cell lysate was separated by 16% SDS-PAGE and transferred onto nitrocellulose.
Immunohistochemistry of S100A1/S100B polyclonal antibody ( Cat # PAB11341 ) was used at a 1:500 dilution to detect S100A1/S100B.Dark nuclear staining is observed within basket cells located near the Purkinje cells in the mouse cerebellum.Personal Communication, Teri Johnson, Stowers Institute, Kansas City, MO.
Immunohistochemical staining of S100A1 on formalin fixed, paraffin embedded human melanoma with S100A1 polyclonal antibody.
Human melanoma stained with S100A1 polyclonal antibody ( Cat # PAB12732 ) at 1 : 300 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
Formalin-fixed and paraffin-embedded human breast tissue (C; 4 microns) stained with anti-S100 showing strong positive immunostaining of the outer myoepithelial cell component in mammary lobule.
Staining with S100 Antibody (22520)
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded.
Formalin-Fixed, Paraffin-Embedded Human neurofibroma tissue (A, 4 μm), skin melanoma tissue (B, 4 μm), and human breast tissue (C, 4 μm) stained with anti-S100 antibody (DB 055) show: positive immunostaining of tumor cells and peripheral nerve (A), strong positive immunostaining of melanoma cells (B) and strong positive immunostaining of the outer myoepithelial cell component in mammary lobule (C). Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human neurofibroma tissue (A, 4 μm), skin melanoma tissue (B, 4 μm), and human breast tissue (C, 4 μm) stained with anti-S100 antibody (DB 055) show: positive immunostaining of tumor cells and peripheral nerve (A), strong positive immunostaining of melanoma cells (B) and strong positive immunostaining of the outer myoepithelial cell component in mammary lobule (C). Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human neurofibroma tissue (A, 4 μm), skin melanoma tissue (B, 4 μm), and human breast tissue (C, 4 μm) stained with anti-S100 antibody (DB 055) show: positive immunostaining of tumor cells and peripheral nerve (A), strong positive immunostaining of melanoma cells (B) and strong positive immunostaining of the outer myoepithelial cell component in mammary lobule (C). Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Formalin-Fixed, Paraffin-Embedded Human neurofibroma tissue (A, 4 μm), skin melanoma tissue (B, 4 μm), and human breast tissue (C, 4 μm) stained with anti-S100 antibody (DB 055) show: positive immunostaining of tumor cells and peripheral nerve (A), strong positive immunostaining of melanoma cells (B) and strong positive immunostaining of the outer myoepithelial cell component in mammary lobule (C). Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic.
Recombinant mouse S100A1, 1-94 aa, His-tagged
Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human brain tissue using S100A1 Antibody (C-term) Cat.-No AP53776PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of S100A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis in MDA-MB435 cell line lysates (35 ug/lane) using S100A1 Antibody (Center) Cat.-No AP53776PU-N. This demonstrates the S100A1 antibody detected the S100A1 protein (arrow).
Immunohistochemical analysis of paraffin-embedded human brain tissue (A), lymphoid follicles tissue (B) and interbrain tissue (C), showing cytoplasmic localization using S100A antibody Cat.-No AM06160SU-N with DAB staining.
Western blot analysis using S100A antibody Cat.-No AM06160SU-N against truncated S100A recombinant protein.
Formalin-Fixed, Paraffin-Embedded Human melanoma stained with S100 Antibody Cat.-No AM32832PU (Clone 4C4.9).
Western blot analysis of Human brain lysate using S100 Antibody at 1 µg/ml.
Formalin-Fixed, Paraffin-Embedded Human melanoma stained with S100 Antibody Cat.-No AM32832PU (Clone 4C4.9).
Formalin-fixed, paraffin-embedded human melanoma stained with S100 Antibody Cat.-No AM50167PU (Clone SPM354).
Western blot analysis of human brain lysate using S100 Antibody Cat.-No AM50167PU (Clone SPM354).
Immunofluorescent analysis of S100-A1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of S100-A1 expression in HEK293T (A); mouse brain (B); rat brain (C); C6 (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-S100A1 Rabbit Polyclonal antibody. (TA590223)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-S100A1 Rabbit Polyclonal antibody. (TA590223)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-S100A1 Rabbit Polyclonal antibody. (TA590223)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-S100A1 Rabbit Polyclonal antibody. (TA590223)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A1 (RC200278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A1 (RC200278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A1.
Standard curve for ELISA analysis with S100A1 recombinant protein (dilution range from 100pg/ml to 1µg/ml) using S100A1 Capture Antibody (Cat# TA801079) at 5µg/ml and HRP conjugated S100A1 Detection mAb (Cat# TA800811) at 0.033µg/ml.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A1 (RC200278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A1.
Standard curve for ELISA analysis with S100A1 recombinant protein (dilution range from 100pg/ml to 1µg/ml) using S100A1 Capture Antibody (Cat# TA801079) at 5µg/ml and HRP conjugated S100A1 Detection mAb (Cat# TA800811) at 0.033µg/ml.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-S100A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807339)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A1 (RC200278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-S100A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807374)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A1 (RC200278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A1.
Western blot analysis of extracts from A549 cells, using S100 A1 antibody (#TA312311).The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells (Lane 2), using S100 A1 Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using S100 A1 antibody (#TA312311).The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using S100 A1 antibody (#TA312311).The picture on the right is treated with the synthesized peptide.
WB using Anti-S-100 antibody shows detection of a band ~11 kDa corresponding to bovine S-100 monomer (100 ng loaded, arrowhead lane 1). The antibody also detects S-100 from rat brain lysate (lane 2). The primary antibody was diluted to 1:1,000 for 2h at RT, followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature.
Rabbit anti-S-100 protein was used at 1:500 to detect S-100 by IHC using a 2-step indirect method. Dark nuclear staining is observed within basket cells located near the Purkinje cells in the cerebellum. Primary antibody was diluted as stated and reacted for 30' followed by washes and the addition of donkey anti-rabbit HRP diluted 1:500 for 30'. DAB+ (Dakocytomation) was used as a substrate and was allowed to react for 5'.
Rabbit anti-S100 was used at a 1:500 dilution to detect S100 by immunohistochemistry in human brain astrocyte tumor tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/50-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using S100A1 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using S100A1 antibody at dilution 1/70, on the right is treated with the synthetic peptide. (Original magnification:x200)
Immunohistochemistry staining of Paraffin Melanoma tissue by S-100 antibody (dilution: 1:50 - 1:200; visualization of staining: Cytoplasmic, nuclear)
Western blot analysis on A549 cell lysate using S100 A1 Antibody
Western blot analysis of S100-A1 expression in 293T (A); mouse brain (B) whole cell lysates.
Immunohistochemical analysis of S100-A1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (S100A1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using (S100A1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM32830PU-S | S100 antibody |
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Mouse | IgG2a | 4C4.9 | Purified | Bov, Hu, Ms, Rt | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
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| OriGene Technologies GmbH | |||||||
 +2 additional images |
Published recently! OriGene recommends this product for Immunohistochemistry. |
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| AM32830PU-T | S100 antibody |
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Mouse | IgG2a | 4C4.9 | Purified | Bov, Hu, Ms, Rt | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
Published recently! OriGene recommends this product for Immunohistochemistry. |
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| TA312311 | S100A1 antibody |
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| Rabbit polyclonal anti-S100 A1 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA319193 | S100A1 antibody |
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| Rabbit polyclonal S100 Protein antibody | |||||||
![WB using Anti-S-100 antibody shows detection of a band ~11 kDa corresponding to bovine S-100 monomer (100 ng loaded, arrowhead lane 1). The antibody also detects S-100 from rat brain lysate (lane 2). The primary antibody was diluted to 1:1,000 for 2h at RT, followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/200-401-874-1-W.jpg) |
Rabbit | IgG | Aff - Purified | Bov | E, P, WB |
0.5 mg /
€590.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA321757 | S100A1 antibody |
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| Anti-S100A1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA321758 | S100A1 antibody |
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| Anti-S100A1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA327733 | S100A1 antibody |
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| Mouse Monoclonal S-100 Antibody | |||||||
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Mouse | IgG2a | 4C4.9 | Purified | Hu | P |
1 ml /
€445.00
|
| OriGene Technologies, Inc. | |||||||
| TA347469 | S100A1 antibody |
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| Rabbit Polyclonal Anti-S100 A1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA349435 | S100A1 antibody |
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| Rabbit Polyclonal Anti-S100A1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA800811 | S100A1 antibody |
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| S100A1 (S100 alpha) mouse monoclonal antibody, clone OTI6G4 (formerly 6G4) | |||||||
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Mouse | IgG1 | OTI6G4 | Purified | Hu | E, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA801079 | S100A1 antibody |
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| S100A1 (S100 alpha) mouse monoclonal antibody, clone OTI9F3 (formerly 9F3) | |||||||
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Mouse | IgG1 | OTI9F3 | Purified | Hu | E, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA807339 | S100A1 antibody |
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| S100A1 mouse monoclonal antibody, clone OTI7B11 (formerly 7B11) | |||||||
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Mouse | IgG1 | OTI7B11 | Purified | Hu | P, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA807374 | S100A1 antibody |
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| S100A1 mouse monoclonal antibody, clone OTI2A4 (formerly 2A4) | |||||||
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Mouse | IgG1 | OTI2A4 | Purified | Hu | P, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AM06160SU-N | S100A1 antibody |
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Mouse | IgG1 | 2C8B8 | Ascites | Hu | P, WB |
0.1 ml /
€380.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AM08285PU-N | S100A1 (C-term) antibody |
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Rabbit | Ig | Purified | Hu | P |
0.1 ml /
€425.00
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| OriGene Technologies GmbH | |||||||
| AP06310PU-N | S100A1 antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€370.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP07684PU-N | S100A1 (Full Length) antibody |
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Rabbit | Purified | Bov, Hu | E, P, WB |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
| AP53776PU-N | S100A1 (C-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| 4S37-3B10 | S100A1 (ab) antibody |
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Mouse | IgG2a | 3B10 | Purified | Hu | E, E(capture), WB |
1 mg /
€420.00
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| HyTest Ltd. | |||||||
| 4S37-6G1 | S100A1 (bb+ab) antibody |
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Mouse | IgG1 | 6G1 | Purified | Hu | E, E(detection), WB |
1 mg /
€420.00
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| HyTest Ltd. | |||||||
