SAMHD1 antibody
Principal name
SAMHD1 antibody
Alternative names for SAMHD1 antibody
DCIP, Monocyte protein 5, MOP5, MOP-5, SAM domain and HD domain-containing protein 1, Dendritic cell-derived IFNG-induced protein
SwissProt ID
Q0VCA5 (Bovin), Q502K2 (Danre), Q5ZJL9 (Chick), Q60710 (Mouse), Q6INN8 (Xenla), Q9Y3Z3 (Human)
Ncbi ID
9606, NP_056289, NP_056289.2, NP_001132992.1, NM_018851, NM_001139520, NM_015474
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P)
Background of SAMHD1 antibody
- Kit Component:
- - KN315281G1, Samhd1 gRNA vector 1 in pCas-Guide vector
- KN315281G2, Samhd1 gRNA vector 2 in pCas-Guide vector
- KN315281D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Olsen,J.V., (2006) Cell 127 (3), 635-648
Immunofluorescence of purified MaxPab antibody to SAMHD1 on HeLa cell. [antibody concentration 10 ug/ml]
SAMHD1 MaxPab polyclonal antibody. Western Blot analysis of SAMHD1 expression in human liver.
Western Blot analysis of SAMHD1 expression in transfected 293T cell line (H00025939-T01) by SAMHD1 MaxPab polyclonal antibody.Lane 1: SAMHD1 transfected lysate(68.86 KDa).Lane 2: Non-transfected lysate.
Human 721_B; WB Suggested Anti-SAMHD1 Antibody Titration: 0.2-1 ug/ml. . Positive Control: 721_B cell lysate; SAMHD1 Antibody - middle region (ARP55262_P050) in Human 721_B cells using Western Blot
Human HepG2; WB Suggested Anti-SAMHD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; SAMHD1 Antibody - middle region (ARP55262_P050) in Human HepG2 cells using Western Blot
Figure from citation: Immunofluorescence of SAMHD1 protein level by using anti-SAMHD1 antibody in human CD4+ T cells. View Citation
Anti-SAMHD1 mouse monoclonal antibody (TA501953) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1(RC206013).
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SAMHD1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.
HEK293T cells transfected with either RC206013 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SAMHD1 antibody(TA501953), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody(TA501953),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-SAMHD1 mouse monoclonal antibody (TA501954) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1(RC206013).
Figure from citation: Western Blot of SAMHD1 protein level by using anti-SAMHD1 antibody in lysates from parental U937 or U937 expressing human or mouse SAMHD1. Dilution: 1:1000 View Citation
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-SAMHD1 monoclonal antibody at 1:200 dilution.
Flow cytometric Analysis of Jurkat cells, using anti-SAMHD1 antibody(TA501954),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody(TA501954),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-SAMHD1 monoclonal antibody at 1:200 dilution.
Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody(TA501955),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501956)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501956)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501956)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501956)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501956)
Anti-SAMHD1 mouse monoclonal antibody (TA501956) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1(RC206013).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502024)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502024)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502024)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502024)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502024)
Anti-SAMHD1 mouse monoclonal antibody (TA502024) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1(RC206013).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody(TA502024),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-SAMHD1 antibody(TA502024),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-SAMHD1 mouse monoclonal antibody (TA502025) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1(RC206013).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.
Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody(TA502025),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-SAMHD1 antibody(TA502025),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-SAMHD1 mouse monoclonal antibody (TA502050) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1(RC206013).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-SAMHD1 mouse monoclonal antibody (TA502085) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1(RC206013).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody(TA502085),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502157)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502157)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502157)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502157)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502157)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502157)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502157)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502157)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502157)
Anti-SAMHD1 mouse monoclonal antibody (TA502157) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1(RC206013).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SAMHD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody(TA502157),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC206013 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SAMHD1 antibody(TA502157), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-SAMHD1 antibody(TA502157),(Red), compared to a nonspecific negative control antibody,(Blue).
Sample (30 ug of whole cell lysate). A: THP-1. 7.5% SDS PAGE. TA308362 diluted at 1:500.
SAMHD1 antibody [N2C2], Internal detects SAMHD1 protein at nucleus on SkHep1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1 xenograft. SAMHD1 antibody [N2C2], Internal (TA308362) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SAMHD1(TA308362) antibody at 1:500 dilution.
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN206013 | SAMHD1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206013BN | SAMHD1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206013LP | SAMHD1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206013RB | SAMHD1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||