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SAMSN1 antibody

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Principal name

SAMSN1 antibody

Alternative names for SAMSN1 antibody

SAMSN-1, SH3-SAM adaptor protein, HACS1, SAM domain-containing protein SAMSN-1, SAM domain SH3 domain and nuclear localization signals protein 1, Hematopoietic adaptor containing SH3 and SAM domains 1

SwissProt ID

P57725 (Mouse), Q9NSI8 (Human)

Gene ID

64092 (SAMSN1), 67742 (Samsn1)

Ncbi ID

9606, NP_071419, NM_022136, NP_071419.3, NP_075869.2, NM_023380

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of SAMSN1 antibody

SAMSN1 is a member of a novel gene family of putative adaptors and scaffold proteins containing SH3 and SAM (sterile alpha motif) domains (Claudio et al., 2001 [PubMed 11536050]).[supplied by OMIM, Mar 2008]. Transcript Variant: This variant (2) contains alterte exons at the 5' end and initiates translation from an alterte start codon compared to variant 1. The resulting longer isoform (2) has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordites used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combition :: AF218085.2 [ECO:0000332] Rseq introns :: mixed/partial sample support ERS025081, ERS025082 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

General readings

Uchida T, Nakao A, Nakano N, et al. Identification of Nash1, a novel protein containing a nuclear localization signal, a sterile alpha motif, and an SH3 domain preferentially expressed in mast cells. Biochem. Biophys. Res. Commun. 2001; 288:137-41.
Claudio JO, Zhu YX, Benn SJ, et al. HACS1 encodes a novel SH3-SAM adaptor protein differentially expressed in normal and malignant hematopoietic cells. Oncogene 2001; 20:5373-7.
Zhu YX, Benn S, Li ZH, et al. The SH3-SAM adaptor HACS1 is up-regulated in B cell activation signaling cascades. J. Exp. Med. 2004; 200:737-47.
Wang D, Stewart AK, Zhuang L, et al. Enhanced adaptive immunity in mice lacking the immunoinhibitory adaptor Hacs1. FASEB J. 2010; 24:947-56.

H00064092-B01 - SAMSN1
Western Blot analysis of SAMSN1 expression in transfected 293T cell line (H00064092-T01) by SAMSN1 MaxPab polyclonal antibody.Lane 1: SAMSN1 transfected lysate(41.03 KDa).Lane 2: Non-transfected lysate.
PAB6673 - SAMSN1
SAMSN1 polyclonal antibody ( Cat # PAB6673 ) ( 1 µg/ml ) staining of Jurkat lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
H00064092-B01P - SAMSN1
Western Blot analysis of SAMSN1 expression in transfected 293T cell line (H00064092-T01) by SAMSN1 MaxPab polyclonal antibody.Lane 1: SAMSN1 transfected lysate(41.03 KDa).Lane 2: Non-transfected lysate.
ARP45875_P050 - SAMSN1
Human Muscle; WB Suggested Anti-SAMSN1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Muscle; SAMSN1 antibody - middle region (ARP45875_P050) in Human Muscle cells using Western Blot
ARP45874_P050 - SAMSN1
Human MCF-7; WB Suggested Anti-SAMSN1 Antibody Titration: 0.2-1 ug/ml. Positive Control: MCF7 cell lysate; SAMSN1 antibody - middle region (ARP45874_P050) in Human MCF-7 cells using Western Blot
AR51545PU-N - SAMSN1
TA808899 - SAMSN1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMSN1 (RC203721, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMSN1.(1:2000)
TA808900 - SAMSN1
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SAMSN1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808900)(1:150)
TA808900 - SAMSN1
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SAMSN1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808900)(1:150)
TA808900 - SAMSN1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMSN1 (RC203721, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMSN1.(1:2000)
TA808901 - SAMSN1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMSN1 (RC203721, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMSN1.(1:2000)
TP760085 - SAMSN1
TA302597 - SAMSN1
TA302597 (1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA319998 - SAMSN1
Western blot analysis of SAMSN in Hela cell lysate with SAMSN antibody at (A) 0.5 and (B) 1 ug/mL.
TA319998 - SAMSN1
Immunocytochemistry of SAMSN1 in HeLa cells with SAMSN1 antibody at 2.5 ug/mL.
TA319998 - SAMSN1
Immunofluorescence of SAMSN1 in HeLa cells with SAMSN1 antibody at 5 ug/mL.
TA338944 - SAMSN1
WB Suggested Anti-SAMSN1 Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate
TA338945 - SAMSN1
WB Suggested Anti-SAMSN1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SAMSN1 (particular isoform) antibody
  Goat Polyclonal Antibody against SAMSN1  
TA302597 (1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

SAMSN1 (C-term) antibody
  Rabbit Polyclonal SAMSN Antibody  
Western blot analysis of SAMSN in Hela cell lysate with SAMSN antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

SAMSN1 antibody
  SAMSN1 mouse monoclonal antibody, clone OTI2D8 (formerly 2D8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMSN1 (RC203721, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMSN1.(1:2000) Mouse IgG1 OTI2D8 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

SAMSN1 antibody
  SAMSN1 mouse monoclonal antibody, clone OTI7F5 (formerly 7F5)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SAMSN1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808900)(1:150) Mouse IgG1 OTI7F5 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+2 additional images

TrueMAB

SAMSN1 antibody
  SAMSN1 mouse monoclonal antibody, clone OTI7C12 (formerly 7C12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMSN1 (RC203721, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMSN1.(1:2000) Mouse IgG1 OTI7C12 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SAMSN1 (full length, N-term HIS tag)
  Recombinant protein of human SAM domain, SH3 domain and nuclear localization signals 1 (SAMSN1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
SAMSN1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SAMSN1 overexpression lysate
SAMSN1 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Samsn1 (GFP-tagged) - Mouse SAM domain, SH3 domain and nuclear localization signals, 1 (Samsn1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Samsn1 (GFP-tagged) - Mouse SAM domain, SH3 domain and nuclear localization signals, 1 (Samsn1), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Samsn1 (Myc-DDK-tagged) - Mouse SAM domain, SH3 domain and nuclear localization signals, 1 (Samsn1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Samsn1 (Myc-DDK-tagged) - Mouse SAM domain, SH3 domain and nuclear localization signals, 1 (Samsn1), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

9 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (5)
  4. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (3)
  2. Polyclonal (2)
Target
  1. SAMSN1 (6)
Property
  1. C-term (1)
  2. particular isoform (1)
Reactivity
  1. Human (Hu) (5)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Enzyme Immunoassay (E) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Purified (3)
  2. Aff - Purified (2)
Host
  1. Mouse (3)
  2. Goat (1)
  3. Rabbit (1)
Images
  1. 1 image (6)
  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Immunofluorescence (IF) (1)
  3. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. E. coli (1)
Manufacturer
  1. OriGene Technologies (9)
Service
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