SAR1A antibody
Principal name
SAR1A antibody
Alternative names for SAR1A antibody
SAR1, SARA, SARA1, GTP-binding protein SAR1a, COPII-associated small GTPase
Gene ID
56681 (SAR1A)
Ncbi ID
9606, NP_064535, NP_001136120, NP_001136120.1, NM_020150, NM_001142648
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F)
Background of SAR1A antibody
- Kit Component:
- - KN201450G1, SAR1A gRNA vector 1 in pCas-Guide vector
- KN201450G2, SAR1A gRNA vector 2 in pCas-Guide vector
- KN201450D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Bi X, Mancias JD, Goldberg J.
Insights into COPII coat nucleation from the structure of Sec23.Sar1 complexed with the active fragment of Sec31.
with the active fragment of Sec31.
Dev Cell. 2007 Nov;13(5):635-45.
PMID: 17981133
Western Blot analysis of SAR1A expression in transfected 293T cell line (H00056681-T01) by SAR1A MaxPab polyclonal antibody.Lane 1: SAR1A transfected lysate(21.89 KDa).Lane 2: Non-transfected lysate.
SAR1A monoclonal antibody (M01), clone 3G5. Western Blot analysis of SAR1A expression in Jurkat ( Cat # L017V1 ).
Western Blot analysis of SAR1A expression in transfected 293T cell line (H00056681-T02) by SAR1A MaxPab polyclonal antibody.Lane 1: SAR1A transfected lysate(21.78 KDa).Lane 2: Non-transfected lysate.
Recombinant human SAR1A, aa 1-198, His-tagged: 15% SDS-PAGE (3 µg)
SAR1A polyclonal antibody ( Cat # PAB11416 ) ( 0.1 µg/ml ) staining of NIH/3T3 lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
TA305682 (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of lysates from 293,HT1080 cell line,human placenta,mouse liver and lung tissue (from left to right),using SAR1A Antibody (Center)(Cat. #TA324879).TA324879 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
Formalin-fixed and paraffin-embedded human lung carcinoma with SAR1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of NCI-H292 cells using SAR1A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA305682 | SAR1A antibody |
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| Goat Anti-SAR1 Antibody | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA324879 | SAR1A (Center) antibody |
||||||
| Rabbit polyclonal SAR1A Antibody (Center) | |||||||
 |
Rabbit | Hu | F, P, WB |
0.4 ml /
€415.00
|
|||
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301450 | SAR1A (transcript variant 2) |
||||
| Recombinant protein of human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP326577 | SAR1A (transcript variant 1) |
||||
| Recombinant protein of human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||