SATB1 antibody
Principal name
SATB1 antibody
Alternative names for SATB1 antibody
DNA-binding protein SATB1, Special AT-rich sequence-binding protein 1
Gene ID
6304 (SATB1)
Ncbi ID
NP_002962, 9606, NP_001124482.1, NP_001124482, NP_002962.1, ABM85984.1, Q01826, NM_001131010, NM_001195470
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of SATB1 antibody
Human special AT-rich sequence-binding protein-1 (SATB1) is a nuclear matrix/scaffold-associated region DNA-binding protein, predominantly expressed in the thymus and pre-B cells. Like its homolog SATB2, SATB1 selectively binds double-stranded, special AT-rich DNA sequences in which one strand exclusively consists of well-mixed A, T, and C nucleotides. SATB1 contains a dimerization domain that shares similarity with the PDZ motif, identified as an indispensable element for high-affinity binding of SATB1 to DNA. SATB1 constitutes a functional nuclear architecture that has a \'cage-like\' protein distribution surrounding heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex) and transcription factors recruitment. SATB1 functions as a \'genome organizer\' essential for proper T-cell development. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic, and when ectopically expressed in non-metastatic cells, can induce invasive activity in vivo. At least two isoforms of SATB1 are known to exist.
General readings
Tan JA, Sun Y, Song J, Chen Y, Krontiris TG, Durrin LK. SUMO conjugation to the matrix attachment region-binding protein, special AT-rich sequence-binding protein-1 (SATB1), targets SATB1 to promyelocytic nuclear bodies where it undergoes caspase cleavage. The Journal of biological chemistry 2008 Jun 283 (26): 18124-34. PMID: 18408014
Transfected 293T; WB Suggested Anti-SATB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Transfected 293T; SATB1 antibody - C-terminal region (ARP33362_P050) in Transfected 293T cells using Western Blot
Jurkat; Host: Rabbit. Target Name:SATB1. Sample Tissue:Jurkat. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:0.625ug/mL. Peptide Concentration: 1.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 6%-18%. SATB1 is supported by BioGPS gene expression data to be expressed in Jurkat.; SATB1 antibody - C-terminal region (ARP38208_T100) in Jurkat cells using Western Blot
Human Jurkat; WB Suggested Anti-SATB1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. SATB1 is supported by BioGPS gene expression data to be expressed in Jurkat.; SATB1 antibody - C-terminal region (ARP38208_T100) in Human Jurkat cells using Western Blot
Immunohistochemistry of SATB1 in human brain with SATB1 polyclonal antibody ( Cat # PAB13379 ) at 5 µg/ml.
Western blot analysis of SATB1 in A-20 cell lysate with SATB1 polyclonal antibody ( Cat # PAB13379 ) at ( A ) 2 and ( B ) 4 µg/ml.
Western blot analysis of SATB1 in SK-N-SH cell lysate with SATB1 polyclonal antibody ( Cat # PAB13389 ) at ( A ) 1 and ( B ) 2 µg/ml.
Human Thymus (formalin-fixed, paraffin-embedded) stained with SATB1 antibody AP22599PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis of SATB1 in A20 cell lysate with SATB1 antibody at (A) 2 and (B) 4 µg/ml.
AP23696PU-N SATB1 Antibody staining of of paraffin embedded Human Thymus at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
AP23696PU-N SATB1 Antibody staining of MOLT4 lysate at 0.3 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500522, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500522, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500522, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500522, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500522, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500522, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500522, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500522, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500522, Dilution 1:50)
Anti-SATB1 mouse monoclonal antibody (TA500522) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1(RC200421).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SATB1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500523, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500523, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500523, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500523, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500523, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500523, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500523, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500523, Dilution 1:50)
Anti-SATB1 mouse monoclonal antibody (TA500523) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1(RC200421).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500524, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500524, Dilution 1:50)
Anti-SATB1 mouse monoclonal antibody (TA500524) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1(RC200421).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.
Anti-SATB1 mouse monoclonal antibody (TA500547) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1(RC200421).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SATB1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500552, Dilution 1:50)
Anti-SATB1 mouse monoclonal antibody (TA500552) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1(RC200421).
Immunofluorescent staining of HT29 cells using anti-SATB1 mouse monoclonal antibody (TA500552).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500578)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500578)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500578)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500589)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500589)
Anti-SATB1 mouse monoclonal antibody (TA500589) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1(RC200421).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.
Flow cytometric Analysis of Jurkat cells, using anti-SATB1 antibody(TA500589),(Red), compared to a nonspecific negative control antibody,(Blue).
Western blot analysis of SATB1 in A20 cell lysate with SATB1 antibody at (A) 2 and (B) 4 µg/ml. Below: Immunohistochemistry of SATB1 in human brain with SATB1 antibody at 5 µg/ml.
Immunohistochemistry of SATB1 in human brain with SATB1 antibody at 5 ug/ml.
Immunofluorescence of SATB1 in Human Brain cells with SATB1 antibody at 20 ug/mL.
Western blot analysis of SATB1 in SK-N-SH cell lysate with SATB1 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry of SATB1 in human brain tissue with SATB1 antibody at 2.5 ug/ml.
Immunofluorescence of SATB1(NT) in Human Brain cells with SATB1(NT) antibody at 20 ug/mL.
Western blot analysis of extracts of various cell lines, using SATB1 antibody.
Immunofluorescence analysis of A549 cell using SATB1 antibody. Blue: DAPI for nuclear staining.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP23696PU-N | SATB1 antibody |
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Goat | Aff - Purified | Bov, Hu, Ms, Rt | E, P, WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
OriGene recommends this product for Western blotting. |
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| TA306588 | SATB1 (C-term) antibody |
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| Rabbit Polyclonal SATB1 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA306604 | SATB1 (N-term) antibody |
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| Rabbit Polyclonal SATB1 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA332923 | SATB1 antibody |
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| Rabbit anti-SATB1 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP22599PU-N | SATB1 (C-term) antibody |
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 |
Rabbit | Purified | Hu, Ms, Rt | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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