SBDS antibody
Principal name
SBDS antibody
Alternative names for SBDS antibody
Shwachman-Bodian-Diamond syndrome protein, Ribosome maturation protein SBDS
SwissProt ID
A5D8M6 (Xenla), P70122 (Mouse), Q23202 (Caeel), Q3SWZ6 (Bovin), Q5RK30 (Rat), Q5ZIY4 (Chick), Q9Y3A5 (Human)
Ncbi ID
NP_057122, NM_016038, NP_057122.2, NP_075737.1, NM_023248, NM_001008289
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)
Background of SBDS antibody
- Kit Component:
- - KN315315G1, Sbds gRNA vector 1 in pCas-Guide vector
- KN315315G2, Sbds gRNA vector 2 in pCas-Guide vector
- KN315315D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Toöviainen-Salo S., et al (2010) Duodecim. 126(14):1711-9.
Hesling C., et al (2007) Exp. Cell Res. 313:4180-4195.
Human kidney; SBDS antibody - N-terminal region (ARP45719_T100) in Human kidney cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-SBDS Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate; SBDS antibody - N-terminal region (ARP45719_T100) in Human Jurkat cells using Western Blot
Human kidney; SBDS antibody - C-terminal region (ARP45721_T100) in Human kidney cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-SBDS Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate; SBDS antibody - C-terminal region (ARP45721_T100) in Human Jurkat cells using Western Blot
Recombinant human SBDS, 1-250aa, His-tagged
ICC/IF analysis of SBDS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human SBDS antibody (1:100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green)
Western blot analysis: The Cell lysates (40ug) were res ol ved by SDS - PAGE, transferred to PVDF membrane and probed with anti human SBDS antibody (1:3000). Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: HeLa cell lysate
Lane 1.: 293T cell lysate
Lane 2.: HeLa cell lysate
WB Suggested Anti-SBDS Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat cell lysate
Human kidney
WB Suggested Anti-SBDS Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate
Human kidney
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM50018PU-N | SBDS (1-250) antibody |
||||||
 |
Mouse | IgG2b | AT1E8 | Purified | Hu | E, ICC/IF, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
| AM50018PU-S | SBDS (1-250) antibody |
||||||
|
Mouse | IgG2b | AT1E8 | Purified | Hu | E, ICC/IF, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308964 | SBDS |
||||
| Recombinant protein of human Shwachman-Bodian-Diamond syndrome (SBDS) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY414234 | SBDS overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203191L2V | Lenti ORF particles, Sbds (GFP-tagged) - Mouse Shwachman-Bodian-Diamond syndrome homolog (human) (Sbds), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203191L1V | Lenti ORF particles, Sbds (Myc-DDK-tagged) - Mouse Shwachman-Bodian-Diamond syndrome homolog (human) (Sbds), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR206953L2V | Lenti ORF particles, Sbds (GFP-tagged ORF) - Rat Shwachman-Bodian-Diamond syndrome homolog (human) (Sbds), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR206953L1V | Lenti ORF particles, Sbds (Myc-DDK-tagged ORF) - Rat Shwachman-Bodian-Diamond syndrome homolog (human) (Sbds), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
