SCARB1 antibody
Principal name
SCARB1 antibody
Alternative names for SCARB1 antibody
SRB1, SR-BI, CLA1, CLA-1, CD36L1, Scavenger receptor class B member 1, CD36 antigen-like 1, CD36 and LIMPII analogous 1, Collagen type I receptor thrombospondin receptor-like 1
Ncbi ID
9606, 10090, NP_001192011.1, NP_005496.4, NP_113729.1, 10116, NM_001082959, NP_005496, NP_001076428, 37999904, NM_016741, NM_031541, NM_005505
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Functional assay (FN), Neutralisation (NEUT), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of SCARB1 antibody
Scavenger receptors have been studied primarily for their ability to bind and internalize modified lipoproteins. They have been found in the development of atherosclerosis and other macrophage-associated functions. Scavenger receptors also function as pattern recognition receptors for a wide variety of pathogens. This finding indicates a potential role in host defense. SR-BI belongs together with CD36 to the class B scavenger receptor family.
SR-BI is a multiligand membrane protein existing in various organs such as the liver and various cell types such as endothelial cells, macrophages, brain cells, Leydig cells and Sertoli cells. SR-BI has been found as a receptor for phospholipids, free and (lipo)protein-bound ApoE, lipid-bound ApoA-I, HDL, hypochlorite-modified LDL and more. In liver, the PDZK-1 (and possible other PDZ domains) of SR-BI has been found to be essential for cell surface expression and, hence, reverse cholesterol transport. In the brain, the presence of SR-BI seems to be involved in the uptake of oxidatively modified lipoproteins and beta-amyloid protein complexed with ApoE, suggesting SR-BI to be an important tool for studies on neurodegenerative disorders. In the testis, SR-BI is expressed in two somatic cell types: Leydig cells and Sertoli cells. SR-BI functions at least partly as a phosphatidyl serine receptor (PSR), enabling Sertoli cells to recognize and phagocytose apoptotic spermatogenic cells at all stages of differentiation.
Western blot analysis of Scarb1 in mouse liver lysate ( 20 µg ) with Scarb1 polyclonal antibody ( Cat# PAB12042 ).
Western blot analysis of Scarb1 in mouse liver lysate ( 20 µg ) with Scarb1 polyclonal antibody at 2 µg/ml ( Cat# PAB12043 ).
Western blot analysis of Scarb1/Scarb2 in 80 µg of total mouse liver lysates using Scarb1/Scarb2 polyclonal antibody ( Cat # PAB12123 ). Lane 1 : wild-type mice, Lane 2 : SR-BI deficient mice.
Western blot analysis of Scarb1 ( 80kDa ) from mouse teste lysate total protein using Scarb1 polyclonal antibody ( Cat #PAB12597 ).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SCARB1 mouse monoclonal antibody. (TA507135)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SCARB1 mouse monoclonal antibody. (TA507135)
Anti-SCARB1 mouse monoclonal antibody (TA507135) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SCARB1(RC210264).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCARB1 (RC210264, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCARB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SCARB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:400
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SCARB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507181)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SCARB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507181)
Immunofluorescent staining of HeLa cells using anti-SCARB1 mouse monoclonal antibody (TA507181).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCARB1 (RC210264, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCARB1.
Anti-SCARB1 mouse monoclonal antibody (TA507188) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SCARB1(RC210264).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCARB1 (RC210264, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCARB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SCARB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Detection of SR-BI in 25 ug of mouse liver lysate, using anti-SRBI at a 1:1,000 dilution, shows distinct band at 82 kDa.
Western Blot: SR-BI Antibody [NB400-104] - Detection of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins.
Staining of adrenal, cortex
Immunohistochemistry: Immunolocalization of SR-BI in adult mink testis using SR-BI Antibody. SR-BI labeling is visible at the surface and along the outline of the large vacuole.
Immunocytochemistry/Immunofluorescence: SRB1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Western blot analysis of SCARB1 in human spleen tissue lysate with SCARB1 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry of SCARB1 in human spleen tissue with SCARB1 antibody at 2.5 ug/ml.
Immunofluorescence of SCARB1 in Human Spleen tissue with SCARB1 antibody at 20 ug/mL.
TA329094 (0.03µg/ml) staining of Human Adrenal Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA329095 (0.01µg/ml) staining of Human Adrenal Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts of various cell lines, using SCARB1 antibody.
Western Blot: SR-BI Antibody [TA336639] - Detection of SR-BI in mouse liver lysate (20 ug) using NB 400-101. ECL detection 5 seconds.
Immunocytochemistry/Immunofluorescence: SR-BI Antibody [TA336639] - SRB1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Flow Cytometry: SR-BI Antibody [TA336639] - SR-BI antibody was tested at 1:400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.
Western Blot: SR-BI Antibody [TA336641] - Detection of SR-BI (80kDa) in mouse testis lysate total protein using TA336641.
Immunocytochemistry/Immunofluorescence: SR-BI Antibody [TA336641] - SR-BI antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: SR-BI/SR-BII Antibody [TA336645] - Detection of RED-1 in 80 ug of total mouse liver lysates. Lane 1: wild-type mice, Lane 2: SR-BI deficient mice.
Immunohistochemistry-Paraffin: SR-BI/SR-BII Antibody [TA336645] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using 5 ug/ml concentration of SR-BI/SR-BII antibody. Specific and expected granular membrane-cytoplasm
Immunocytochemistry/Immunofluorescence: SR-BI/SR-BII Antibody [TA336645] - SR-BI/SR-BII antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Flow Cytometry: SR-BI/SR-BII Antibody [TA336645] - SR-BI/SR-BII antibody was tested at 1:400 in NIH-3T3 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.
Human peripheral blood lymphocytes were stained with purified CLA (clone HECA-452) (filled histogram) or rat IgM isotype control (open histogram), followed by biotinylated anti-rat IgM and Sav-PE.
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 293T cells, PC3 cells, Primary antibody: (SCARB1 Antibody) at dilution 1/260, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (SCARB1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (SCARB1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA301489 | SCARB1 antibody |
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| Rabbit Polyclonal Antibody against SR-BI | |||||||
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Rabbit | Purified | Hst, Hu, Mink, Ms, Rt | WB |
0.1 ml /
€475.00
|
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA507135 | SCARB1 antibody |
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| SCARB1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3) | |||||||
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Mouse | IgG1 | OTI1F3 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA507181 | SCARB1 antibody |
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| SCARB1 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4) | |||||||
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Mouse | IgG1 | OTI1G4 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA306788 | SCARB1 antibody |
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| Rabbit Polyclonal SCARB1 Antibody | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA329094 | SCARB1 (238-250) antibody |
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| SCARB1 / SR-BI (aa238-250) Goat Polyclonal Antibody | |||||||
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Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA329095 | SCARB1 (C-term) antibody |
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| SCARB1 / SR-BI Goat Polyclonal Antibody | |||||||
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Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA332410 | SCARB1 antibody |
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| Rabbit anti-SCARB1 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA336639 | SCARB1 antibody |
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| Rabbit Polyclonal SR-BI Antibody | |||||||
![Western Blot: SR-BI Antibody [TA336639] - Detection of SR-BI in mouse liver lysate (20 ug) using NB 400-101. ECL detection 5 seconds.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB400-101-1-W.jpg) |
Rabbit | Purified | Bov, Hu, Ms, Rt | C, F, ICC/IF, IP, P, WB |
0.1 ml /
€475.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA336641 | SCARB1 antibody |
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| Rabbit Polyclonal SR-BI Antibody | |||||||
![Western Blot: SR-BI Antibody [TA336641] - Detection of SR-BI (80kDa) in mouse testis lysate total protein using TA336641.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB400-113-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rt | ICC/IF, IP, WB |
0.1 ml /
€475.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA336645 | SCARB1 antibody |
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| Rabbit Polyclonal SR-BI/SR-BII Antibody | |||||||
![Western Blot: SR-BI/SR-BII Antibody [TA336645] - Detection of RED-1 in 80 ug of total mouse liver lysates. Lane 1: wild-type mice, Lane 2: SR-BI deficient mice.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB400-134-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rt | C, F, ICC/IF, WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA351049 | SCARB1 antibody |
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| Rabbit Polyclonal Anti-SCARB1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, P |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA351050 | SCARB1 antibody |
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| Rabbit Polyclonal Anti-SCARB1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA507188 | SCARB1 antibody |
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| Purified SCARB1 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1) | |||||||
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Mouse | IgG2b | OTI1E1 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AM26164PU-N | SCARB1 antibody |
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Mouse | IgG1 | SR-BI | Purified | Rt | C, FN |
0.1 mg /
€530.00
|
| OriGene Technologies GmbH | |||||||
| AM32107PU-N | SCARB1 antibody |
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Mouse | IgG1 | 3D12 | Liquid | Rt | C, NEUT |
0.1 mg /
€880.00
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP310264 | SCARB1 (transcript variant 1) |
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| Recombinant protein of human scavenger receptor class B, member 1 (SCARB1), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
| TP318270 | SCARB1 (transcript variant 2) |
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| Recombinant protein of human scavenger receptor class B, member 1 (SCARB1), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY421197 | SCARB1 overexpression lysate |
|
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0.1 mg /
€495.00
|
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| OriGene Technologies, Inc. | ||
| LY425949 | SCARB1 overexpression lysate |
|
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0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208180L2V | Lenti ORF particles, Scarb1 (GFP-tagged) - Mouse scavenger receptor class B, member 1 (Scarb1), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,200.00
|
|
| OriGene Technologies, Inc. | ||
