SCML1 antibody
Principal name
SCML1 antibody
Alternative names for SCML1 antibody
SCML-1, Sex comb on midleg-like protein 1
SwissProt ID
Q9UN30 (Human)
Gene ID
6322 (SCML1)
Ncbi ID
9606, NP_001032624, NP_001032625, NM_001037540, NP_001032629, NP_001032624.1, NM_006746, NM_001037536
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Immunoprecipitation (IP)
Background of SCML1 antibody
- Kit Component:
- - KN205262G1, SCML1 gRNA vector 1 in pCas-Guide vector
- KN205262G2, SCML1 gRNA vector 2 in pCas-Guide vector
- KN205262D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ross,M.T., (2005) Nature 434 (7031), 325-337
Immunoperoxidase of monoclonal antibody to SCML1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1.2 ug/ml]
Western Blot analysis of SCML1 expression in transfected 293T cell line by SCML1 monoclonal antibody (M01), clone 4G3.Lane 1: SCML1 transfected lysate(23.131 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged SCML1 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to SCML1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of SCML1 expression in transfected 293T cell line (H00006322-T01) by SCML1 MaxPab polyclonal antibody.Lane 1: SCML1 transfected lysate(22.88 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SCML1 expression in transfected 293T cell line (H00006322-T02) by SCML1 MaxPab polyclonal antibody.Lane 1: SCML1 transfected lysate(23.10 KDa).Lane 2: Non-transfected lysate.
Human Colon; Immunohistochemistry with Human Colon lysate tissue at an antibody concentration of 5.0ug/ml using anti-SCML1 antibody (ARP35811_P050); SCML1 antibody - middle region (ARP35811_P050) in Human Colon cells using Immunohistochemistry
Human HeLa; WB Suggested Anti-SCML1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Hela cell lysate. SCML1 is supported by BioGPS gene expression data to be expressed in HeLa.; SCML1 antibody - middle region (ARP35811_P050) in Human HeLa cells using Western Blot
Human 293T; Host:Rabbit. Target Name:SCML1. Sample Tissue:Human 293T. Antibody Dilution: 1.0ug/ml. SCML1 is supported by BioGPS gene expression data to be expressed in HEK293T.; SCML1 antibody - N-terminal region (ARP35812_P050) in Human 293T cells using Western Blot
Human HeLa; WB Suggested Anti-SCML1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate. SCML1 is supported by BioGPS gene expression data to be expressed in HeLa.; SCML1 antibody - N-terminal region (ARP35812_P050) in Human HeLa cells using Western Blot
Human Brain; WB Suggested Anti-SCML1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; SCML1 antibody - N-terminal region (ARP38857_P050) in Human Brain cells using Western Blot
Immunoprecipitation of SCML1 transfected lysate using anti-SCML1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SCML1 MaxPab mouse polyclonal antibody (B01) (H00006322-B01).
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged SCML1 is approximately 0.03 ng/ml as a capture antibody.
WB Suggested Anti-SCML1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Hela cell lysate SCML1 is supported by BioGPS gene expression data to be expressed in HeLa
Immunohistochemistry with Human Colon lysate tissue at an antibody concentration of 5.0 ug/ml using anti-SCML1 antibody
WB Suggested Anti-SCML1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Hela cell lysate SCML1 is supported by BioGPS gene expression data to be expressed in HeLa
Host:Rabbit; Target Name:SCML1; Sample Tissue:Human 293T; Antibody Dilution: 1.0 ug/ml SCML1 is supported by BioGPS gene expression data to be expressed in HEK293T
WB Suggested Anti-SCML1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human brain
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM20939PU-N | SCML1 antibody |
||||||
 |
Mouse | IgG2a | 4G3 | Hu | E, P, WB |
50 µg /
€475.00
|
|
| OriGene Technologies GmbH | |||||||
 +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP312954 | SCML1 (transcript variant 1) |
||||
| Purified recombinant protein of Homo sapiens sex comb on midleg-like 1 (Drosophila) (SCML1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP314112 | SCML1 (transcript variant 3) |
||||
| Recombinant protein of human sex comb on midleg-like 1 (Drosophila) (SCML1), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY421922 | SCML1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY425621 | SCML1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |