SEC14L2 antibody
Principal name
SEC14L2 antibody
Alternative names for SEC14L2 antibody
SEC14-like protein 2, Alpha-tocopherol-associated protein, TAP, SPF, C22orf6, KIAA1186, KIAA1658, Supernatant protein factor, Squalene transfer protein
Ncbi ID
NP_036561, 9606, NP_001191133, NP_001191133.1, NP_653103.1, NM_144520, NM_012429
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)
Background of SEC14L2 antibody
- Kit Component:
- - KN315451G1, Sec14l2 gRNA vector 1 in pCas-Guide vector
- KN315451G2, Sec14l2 gRNA vector 2 in pCas-Guide vector
- KN315451D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Collins,J.E., et al., (2004) Genome Biol. 5(10), R84
Human Liver; WB Suggested Anti-SEC14L2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:7812500. Positive Control: Human Liver; SEC14L2 antibody - middle region (ARP31825_P050) in Human Liver cells using Western Blot
SEC14L2 MaxPab polyclonal antibody. Western Blot analysis of SEC14L2 expression in human liver.
Western Blot analysis of SEC14L2 expression in transfected 293T cell line (H00023541-T01) by SEC14L2 MaxPab polyclonal antibody.Lane 1: SEC14L2 transfected lysate(43.12 KDa).Lane 2: Non-transfected lysate.
SEC14L2 MaxPab polyclonal antibody. Western Blot analysis of SEC14L2 expression in human liver.
Western Blot analysis of SEC14L2 expression in transfected 293T cell line (H00023541-T01) by SEC14L2 MaxPab polyclonal antibody.Lane 1: SEC14L2 transfected lysate(43.12 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of SEC14L2 Antibody (N-term) Cat.-No AP53831PU-N in Mouse liver tissue lysates (35ug/lane). This demonstrates the SEC14L2 antibody detected the SEC14L2 protein (arrow).
Anti-SEC14L2 mouse monoclonal antibody (TA503561) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2(RC215994).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (RC215994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (RC215994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SEC14L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503577)
Anti-SEC14L2 mouse monoclonal antibody (TA503577) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2(RC215994).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (RC215994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.
HEK293T cells transfected with either RC215994 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SEC14L2 antibody(TA503577), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (RC215994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SEC14L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503723)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SEC14L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503723)
Anti-SEC14L2 mouse monoclonal antibody (TA503723) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2(RC215994).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (RC215994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SEC14L2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SEC14L2 monoclonal antibody.
HEK293T cells transfected with either RC215994 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SEC14L2 antibody(TA503723), and then analyzed by flow cytometry.
WB Suggested Anti-SEC14L2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:7812500; Positive Control: Human Liver
- Quick-Filters:
- Cited (1)
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP53831PU-N | SEC14L2 (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Bov, Ms, Rb, Rt | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
Published recently! |
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| TA503577 | SEC14L2 antibody |
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| SEC14L2 (ﾠSEC14-like 2) mouse monoclonal antibody, clone OTI1F10 (formerly 1F10) | |||||||
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Mouse | IgG1 | OTI1F10 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
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| TA503723 | SEC14L2 antibody |
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| SEC14L2 (ﾠSEC14-like 2) mouse monoclonal antibody, clone OTI4H2 (formerly 4H2) | |||||||
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Mouse | IgG1 | OTI4H2 | Purified | Can, Hu, Mky, Ms | F, ICC/IF, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
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| TA351640 | SEC14L2 antibody |
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| Rabbit Polyclonal Anti-SEC14L2 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA503561 | SEC14L2 antibody |
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| SEC14L2 (ﾠSEC14-like 2) mouse monoclonal antibody, clone OTI1H3 (formerly 1H3) | |||||||
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Mouse | IgG2b | OTI1H3 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA503576 | SEC14L2 antibody |
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| SEC14L2 (ﾠSEC14-like 2) mouse monoclonal antibody, clone OTI2F9 (formerly 2F9) | |||||||
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Mouse | IgG2b | OTI2F9 | Purified | Hu | WB |
0.1 ml /
€360.00
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| OriGene Technologies, Inc. | |||||||
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| TA503595 | SEC14L2 antibody |
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| SEC14L2 (ﾠSEC14-like 2) mouse monoclonal antibody, clone OTI1B1 (formerly 1B1) | |||||||
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Mouse | IgG1 | OTI1B1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP315994 | SEC14L2 (transcript variant 1) |
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| Recombinant protein of human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206338L2V | Lenti ORF particles, Sec14l2 (GFP-tagged) - Mouse SEC14-like 2 (S. cerevisiae) (Sec14l2), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||
| MR206338L1V | Lenti ORF particles, Sec14l2 (Myc-DDK-tagged) - Mouse SEC14-like 2 (S. cerevisiae) (Sec14l2), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
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| OriGene Technologies, Inc. | ||