SEMA3F antibody
Principal name
SEMA3F antibody
Alternative names for SEMA3F antibody
Semaphorin-3F, Semaphorin IV, Sema IV, Sema III/F
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)
Background of SEMA3F antibody
- Kit Component:
- - KN315498G1, Sema3f gRNA vector 1 in pCas-Guide vector
- KN315498G2, Sema3f gRNA vector 2 in pCas-Guide vector
- KN315498D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
HEK293; . Sample Type :. Untransfected HEK293 and Sema3F-AP transfected HEK293 . Primary Antibody Dilution :. 1:1000 . Secondary Antibody :. Anti rabbit-Alexa Fluor 488 . Secondary Antibody Dilution :. 1:500 . Color/Signal Descriptions :. . Gene Name :. SEMA3F. Submitted by :. Dr. Tracy Tran, Rutgers University; Sema3f antibody - N-terminal region (ARP56569_P050) in HEK293 cells using Immunohistochemistry
HEK293; WB Suggested Anti-SEMA3F Antibody . . Positive Control: Lane1: No transfection, Lane, 2: Cell lysates from HEK293 cells were transfected with alkaline phosphatase (AP-) tagged Sema3F, Lane3: AP-alone, Lane4: The cell media from cells transfected with AP-Sema3F . Primary Antibody Dilution : 1ug/ml . Secondary Antibody : Anti-AP . Secondry Antibody Dilution : 1:1000 . Submitted by: Dr. Tracy Tran, Rutgers University; Sema3f antibody - N-terminal region (ARP56569_P050) in HEK293 cells using Western Blot
Mouse Liver; WB Suggested Anti-Sema3f Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Liver; Sema3f antibody - N-terminal region (ARP56569_P050) in Mouse Liver cells using Western Blot
HEK293F; Researcher: Dr. Chrisna LeVaillant, School of Anatomy and Human Biology. Application: IP. Species+tissue/cell type:1.5mL conditioned media from HEK293F cells transfected with 1: Sema 3F_AP, 2: Sema3C_FLAG, 3: UntransfectedAmount of IP Antibody: Amount of IP Antibody: 5ug. Primary antibody dilution: 1:1000. Secondary antibody: Goat Anti-rabbit HRP. Secondary antibody dilution:1:10000 - See more at: http://www.avivasysbio.com/anti-sema3f-antibody-n-terminal-region-arp56569-p050.html#sthash.vpQY9cNN.dpuf; Sema3f antibody - N-terminal region (ARP56569_P050) in HEK293F cells using Immunoprecipitation
WB Suggested Anti-Sema3f Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Liver
WB Suggested Anti-SEMA3F Antibody ; Positive Control: Lane1: No transfection, Lane, 2: Cell lysates from HEK293 cells were transfected with alkaline phosphatase (AP-) tagged Sema3F, Lane3: AP-alone, Lane4: The cell media from cells transfected with AP-Sem
Sample Type :; Untransfected HEK293 and Sema3F-AP transfected HEK293 Primary Antibody Dilution :; 1:1000 Secondary Antibody :; Anti rabbit-Alexa Fluor 488 Secondary Antibody Dilution :; 1:500 Color/Signal Descriptions :; Gene Name :; SEMA3F
Application: IP; Species+tissue/cell type:1.5mL conditioned media from HEK293F cells transfected with 1: Sema 3F_AP, 2: Sema3C_FLAG, 3: UntransfectedAmount of IP Antibody: Amount of IP Antibody: 5ug; Primary antibody dilution: 1:1000; Secondary antibody:
Lysates
| Catalog No. | ||
|---|---|---|
| LY418156 | SEMA3F overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210479L2V | Lenti ORF particles, Sema3f (GFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (Sema3f), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,550.00
|
|
| OriGene Technologies, Inc. | ||
| MR210479L1V | Lenti ORF particles, Sema3f (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (Sema3f), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,390.00
|
|
| OriGene Technologies, Inc. | ||
