SEMA4F antibody
Principal name
SEMA4F antibody
Alternative names for SEMA4F antibody
Semaphorin-4F, Semaphorin-W, Semaphorin-M, Sema W, Sema M, SEMAW, SEMAM
Ncbi ID
9606, NP_004254, NP_004254.2, ABM86603.1, NM_004263, NP_035480.3, NM_001113481, NM_019272
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of SEMA4F antibody
- Kit Component:
- - KN315504G1, Sema4f gRNA vector 1 in pCas-Guide vector
- KN315504G2, Sema4f gRNA vector 2 in pCas-Guide vector
- KN315504D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Transfected 293T; WB Suggested Anti-SEMA4F Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; SEMA4F antibody - N-terminal region (ARP46420_P050) in Transfected 293T cells using Western Blot
Transfected 293T; WB Suggested Anti-SEMA4F Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; SEMA4F antibody - N-terminal region (ARP46421_P050) in Transfected 293T cells using Western Blot
human A431; human cell line A431. Primary antibody dilution and incubation time:1:600, 4 degree overnightSecondary antibody used and dilution and incubation time: 1:3000, RT 2 hours; SEMA4F antibody - N-terminal region (ARP46421_P050) in human A431 cells using Western Blot
Human MIA-PACA2; . Lanes: 1. Untransfected MIA-PACA2 cell lysate. 2. Untransfected MIA-PACA2 cell lysate. 3. Untransfected MIA-PACA2 cell lysate. 4. hSEMA4F transfected MIA-PACA2 cell lysate. 5. Untransfected MIA-PACA2 cell lysate. 6. hSEMA4F transfected MIA-PACA2 cell lysate. Primary Antibody Dilution: 1:600. Secondary Antibody: Donkey Anti-rabbit HRP. Secondary Antibody Dilution: 1:3000. Gene Name: SEMA4F. Submitted by: Dr. Tianliang Sun, Max Planck Institute for Heart and Lung Research.; SEMA4F antibody - N-terminal region (ARP46421_P050) in Human MIA-PACA2 cells using Western Blot
Human A431; . Lanes: 1. A431 cell lysate + control siRNA 2. A431 cell lysate + SEMA4F siRNA. Primary Antibody Dilution: 1:600. Secondary Antibody: Donkey Anti-rabbit HRP. Secondary Antibody Dilution: 1:3000. Gene Name: SEMA4F. Submitted by: Dr. Tianliang Sun, Max Planck Institute for Heart and Lung Research.; SEMA4F antibody - N-terminal region (ARP46421_P050) in Human A431 cells using Western Blot
WB Suggested Anti-SEMA4F Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T
WB Suggested Anti-SEMA4F Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T
human cell line A431; Primary antibody dilution and incubation time: 1: 600, 4 degree overnight.Secondary antibody used and dilution and incubation time: 1: 3000, RT 2 hours
Lanes: 1. Untransfected MIA-PACA2 cell lysate; 2. Untransfected MIA-PACA2 cell lysate; 3. Untransfected MIA-PACA2 cell lysate; 4. hSEMA4F transfected MIA-PACA2 cell lysate; 5. Untransfected MIA-PACA2 cell lysate; 6. hSEMA4F transfected MIA-PACA2 cell lysa
Lanes: 1. A431 cell lysate + control siRNA 2. A431 cell lysate + SEMA4F siRNA; Primary Antibody Dilution: 1: 600; Secondary Antibody: Donkey Anti-rabbit HRP; Secondary Antibody Dilution: 1: 3000; Gene Name: SEMA4F; Submitted by: Dr. Tianliang Sun, Max Pla
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using (SEMA4F Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (SEMA4F Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA350397 | SEMA4F antibody |
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| Rabbit Polyclonal Anti-SEMA4F Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306544 | SEMA4F |
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| Recombinant protein of human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (SEMA4F) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418105 | SEMA4F overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR221049L2V | Lenti ORF particles, Sema4f (GFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,260.00
|
|
| OriGene Technologies, Inc. | ||
| MR221049L1V | Lenti ORF particles, Sema4f (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain (Sema4f), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,140.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205977L2V | Lenti ORF particles, Sema4f (GFP-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (Sema4f), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,570.00
|
|
| OriGene Technologies, Inc. | ||
| RR205977L1V | Lenti ORF particles, Sema4f (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (Sema4f), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,410.00
|
|
| OriGene Technologies, Inc. | ||