SENP3 antibody
Principal name
SENP3 antibody
Alternative names for SENP3 antibody
Sentrin-specific protease 3, SUSP3, SSP3, Sentrin/SUMO-specific protease SENP3, SUMO-1-specific protease 3
Ncbi ID
9606, NP_056485, NP_056485.2, NM_015670, NP_001157043.1, NM_030702
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of SENP3 antibody
- Kit Component:
- - KN315515G1, Senp3 gRNA vector 1 in pCas-Guide vector
- KN315515G2, Senp3 gRNA vector 2 in pCas-Guide vector
- KN315515D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunohistochemistry (IHC) analyzes of SENP3 antibody (Cat.-No.. AP06458PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of SENP3 antibody (Cat.-No.: AP06458PU-N) in extracts from Jurkat cells.
( LEFT ) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SENP3 polyclonal antibody ( Cat # PAB2416 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. ( RIGHT ) Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SENP3 polyclonal antibody ( Cat # PAB2416 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of SENP3 polyclonal antibody ( Cat # PAB2416 ) in SAOS-2 cell lysate. SENP3 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
(LEFT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT) Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SENP3 antibody (N-term) (Cat.#AP11237PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of SENP3 N-term polyclonal antibody (Cat.#AP11237PU-N) in Saos-2 cell lysate. SENP3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot analysis of SENP3 expression in transfected 293T cell line (H00026168-T02) by SENP3 MaxPab polyclonal antibody.Lane 1: SENP3 transfected lysate(65.00 KDa).Lane 2: Non-transfected lysate.
SENP3 MaxPab rabbit polyclonal antibody. Western Blot analysis of SENP3 expression in HepG2.
Human HeLa; SENP3 antibody - N-terminal region (ARP55322_P050) in HeLa cells using Immunohistochemistry.. SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa.; SENP3 antibody - N-terminal region (ARP55322_P050) in Human HeLa cells using Immunohistochemistry
Rat; . Sample Type: 1. Rat Liver cells (200ug). 2. Rat Brain cells (200ug). 3. Rat Testis cells (200ug) . 4. Rat Kidney cells (200ug). Primary Dilution: 1:1000. Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit. Secondary Dilution: 1:1000. Image Submitted by: Andreia Carvalho. IBMC-OBF, Portugal . See Customer Feedback tab for detailed information.. .; SENP3 antibody - N-terminal region (ARP55322_P050) in Rat cells using Western Blot
Human HeLa; Sample Type: 1. HeLa cell extract (200ug)2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME*HA-S2-VME-SENP3Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.. SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa.; SENP3 antibody - N-terminal region (ARP55322_P050) in Human HeLa cells using Western Blot
Human HeLa; Sample Type: 1. HeLa cell extract (200ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.. SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa.; SENP3 antibody - N-terminal region (ARP55322_P050) in Human HeLa cells using Western Blot
Human 721_B; WB Suggested Anti-SENP3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 721_B cell lysate. SENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; SENP3 antibody - N-terminal region (ARP55322_P050) in Human 721_B cells using Western Blot
Immunohistochemical analysis of SENP3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of SENP3 expression in HeLa (A); MCF7 (B); Saos2 (C) whole cell lysates.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SENP3 antibody.The picture on the right is treated with the synthesized peptide.
Sample Type: 1. Rat Liver cells (200ug); 2. Rat Brain cells (200ug); 3. Rat Testis cells (200ug); 4. Rat Kidney cells (200ug); Primary Dilution: 1: 1000; Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit; Secondary Dilution: 1: 1000; Image S
Sample Type: 1. HeLa cell extract (200ug)Primary Dilution: 1: 1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1: 1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.SENP3 is strongly supported by BioGPS gene e
Sample Type: 1. HeLa cell extract (200ug)2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME*HA-S2-VME-SENP3Primary Dilution: 1: 1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1: 1000Image Submitted by: Andreia
WB Suggested Anti-SENP3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 721_B cell lysateSENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315314 | SENP3 antibody |
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| Rabbit polyclonal anti-SENP3 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA351649 | SENP3 antibody |
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| Rabbit Polyclonal Anti-SENP3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP06458PU-N | SENP3 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AP11237PU-N | SENP3 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY402457 | SENP3 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR224185L1V | Lenti ORF particles, Senp3 (Myc-DDK-tagged) - Mouse SUMO/sentrin specific peptidase 3 (Senp3), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,150.00
|
|
| OriGene Technologies, Inc. | ||