SEPHS1 antibody
Principal name
SEPHS1 antibody
Alternative names for SEPHS1 antibody
Selenide water dikinase 1, Selenophosphate synthase 1, Selenium donor protein 1, SELD, SPS, SPS1
SwissProt ID
O18373 (Drome), P08458 (Yeast), P49903 (Human), Q0VC82 (Bovin), Q6PF47 (Xenla), Q7ZW38 (Danre), Q8BH69 (Mouse)
Ncbi ID
9606, NP_036379, NM_012247, NM_001195604, NP_001182533, NP_036379.2, NP_780609.4, NM_175400, NM_001195602
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of SEPHS1 antibody
- Kit Component:
- - KN315520G1, Sephs1 gRNA vector 1 in pCas-Guide vector
- KN315520G2, Sephs1 gRNA vector 2 in pCas-Guide vector
- KN315520D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Chung,H.J., (2006) J. Cell. Physiol. 209 (1), 131-141
Human HepG2; WB Suggested Anti-SEPHS1 Antibody Titration: 5.0ug/ml. Positive Control: HepG2 cell lysate. SEPHS1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; SEPHS1 antibody - C-terminal region (ARP48737_T100) in Human HepG2 cells using Western Blot
Sample(30 ug of whole cell lysate). A:A431. B:MOLT4. 12% SDS PAGE. TA308432 diluted at 1:500
Selenophosphate synthetase 1 antibody detects SEPHS1 protein at nucleus on Colon Carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded Colon Carcinoma. Selenophosphate synthetase 1 antibody (TA308432) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Selenophosphate synthetase 1(TA308432) antibody at 1:200 dilution.
Western blot using anti-SPS1 antibody shows detection of endogenous SPS1 in NIH3T3 cell. No signal is seen in lysates from cells after pre-treatment with SPS1 siRNA. Endogenous SPS1 can be detected in mouse kidney and liver tissue lysates. Negligible cross-reactivity is seen against recombinant SPS2. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308432 | SEPHS1 antibody |
||||||
| Rabbit polyclonal antibody to Selenophosphate synthetase 1 (selenophosphate synthetase 1) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA319531 | SEPHS1 antibody |
||||||
| Rabbit polyclonal Selenophosphate Synthetase 1 antibody | |||||||
 |
Rabbit | IgG | Serum | Hu, Ms, Rt | E, WB |
0.1 ml /
€515.00
|
|
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309406 | SEPHS1 |
||||
| Recombinant protein of human selenophosphate synthetase 1 (SEPHS1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP710322 | SEPHS1 (full length, C-term DDK tag) |
||||
| Purified recombinant protein of Human aprataxin and PNKP like factor (APLF), full length, with C-terminal DDK tag, expressed in sf9, 20ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|
| OriGene Technologies, Inc. | |||||
| TP760692 | SEPHS1 (full length, N-term HIS tag, transcript variant 3) |
||||
| Purified recombinant protein of Human selenophosphate synthetase 1 (SEPHS1), transcript variant 3, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY415883 | SEPHS1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY434161 | SEPHS1 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206112L2V | Lenti ORF particles, Sephs1 (GFP-tagged) - Mouse selenophosphate synthetase 1 (Sephs1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
