Septin-7 antibody
Principal name
Septin-7 antibody
Alternative names for Septin-7 antibody
SEPT7, SEPT-7, CDC10, CDC10 protein homolog, Septin7
SwissProt ID
O55131 (Mouse), Q16181 (Human), Q5R1W1 (Pantr), Q5R481 (Ponab), Q6Q137 (Bovin), Q9WVC0 (Rat)
Ncbi ID
9606, NP_001011553.2, NP_001779.2, NM_001788, NP_001779, NP_001192296.1, NM_009859, NM_022616, NM_001113740, NM_001011553
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of Septin-7 antibody
- Kit Component:
- - KN315536G1, Sept7 gRNA vector 1 in pCas-Guide vector
- KN315536G2, Sept7 gRNA vector 2 in pCas-Guide vector
- KN315536D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kremer BE, Adang LA, Macara IG.
Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7.
Cell. 2007 Sep 7;130(5):837-50.
PMID: 17803907
Western Blot analysis of SEPT7 expression in transfected 293T cell line (H00000989-T02) by SEPT7 MaxPab polyclonal antibody.Lane 1: SEPT7 transfected lysate(45.98 KDa).Lane 2: Non-transfected lysate.
SEPT7 polyclonal antibody ( Cat # PAB11439 ) ( 0.5 µg/ml ) staining of HeLa nuclear extract ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical analysis of Septin 7 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Septin 7 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Septin 7 expression in MCF7 (A); HepG2 (B); NIH3T3 (C) whole cell lysates.
TA305787 (0.5ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample(30 ug of whole cell lysate). A:HeLa S3. 7.5% SDS PAGE. TA307974 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded GASTRIC CA, using 39331(TA307974) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate). A: MOLT4 . 7.5% SDS PAGE. TA307975 diluted at 1:500
Western blot analysis of extracts from MCF-7 cell, HuvEc cells and HeLa cells, using SEPT7 antibody.The lane on the right is treated with the synthesized peptide.
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: (SEPT7 Antibody) at dilution 1/430, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (SEPT7 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (SEPT7 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA305787 | Septin-7 antibody |
||||||
| Goat Anti-SEPT7 Antibody | |||||||
 |
Goat | Aff - Purified | Bov, Can, Eq, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA307974 | Septin-7 antibody |
||||||
| Rabbit Polyclonal antibody to SEPT7 (septin 7) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA307975 | Septin-7 antibody |
||||||
| Rabbit Polyclonal antibody to SEPT7 (septin 7) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA313584 | Septin-7 antibody |
||||||
| Rabbit polyclonal anti-SEPT7 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349782 | Septin-7 antibody |
||||||
| Rabbit Polyclonal Anti-SEPT7 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN205163 | SEPT7 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205163BN | SEPT7 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205163LP | SEPT7 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205163RB | SEPT7 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305163 | Septin-7 (transcript variant 1) |
||||
| Recombinant protein of human septin 7 (SEPT7), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419745 | SEPT7 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206973L2V | Lenti ORF particles, Sept7 (GFP-tagged) - Mouse septin 7 (Sept7), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,100.00
|
|
| OriGene Technologies, Inc. | ||
| MR206973L1V | Lenti ORF particles, Sept7 (Myc-DDK-tagged) - Mouse septin 7 (Sept7), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€990.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR200310L2V | Lenti ORF particles, Sept7 (GFP-tagged ORF) - Rat septin 7 (Sept7), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,090.00
|
|
| OriGene Technologies, Inc. | ||
| RR200311L2V | Lenti ORF particles, Sept7 (GFP-tagged ORF) - Rat septin 7 (Sept7), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,100.00
|
|
| OriGene Technologies, Inc. | ||
| RR200310L1V | Lenti ORF particles, Sept7 (Myc-DDK-tagged ORF) - Rat septin 7 (Sept7), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€980.00
|
|
| OriGene Technologies, Inc. | ||
| RR200311L1V | Lenti ORF particles, Sept7 (Myc-DDK-tagged ORF) - Rat septin 7 (Sept7), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€980.00
|
|
| OriGene Technologies, Inc. | ||