SERPINB1 antibody
Principal name
SERPINB1 antibody
Alternative names for SERPINB1 antibody
Serpin B1, Leukocyte elastase inhibitor, LEI, ELANH2, MNEI, PI2, PI-2, Monocyte/neutrophil elastase inhibitor, M/NEI, Peptidase inhibitor 2
Gene ID
1992 (SERPINB1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of SERPINB1 antibody
- Kit Component:
- - KN202138G1, SERPINB1 gRNA vector 1 in pCas-Guide vector
- KN202138G2, SERPINB1 gRNA vector 2 in pCas-Guide vector
- KN202138D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of SERPINB1 expression in transfected 293T cell line by SERPINB1 monoclonal antibody (M02), clone 4D7.Lane 1: SERPINB1 transfected lysate(42.7 KDa).Lane 2: Non-transfected lysate.
SERPINB1 MaxPab polyclonal antibody. Western Blot analysis of SERPINB1 expression in human pancreas.
Western Blot analysis of SERPINB1 expression in transfected 293T cell line (H00001992-T02) by SERPINB1 MaxPab polyclonal antibody.Lane 1: SERPINB1 transfected lysate(41.69 KDa).Lane 2: Non-transfected lysate.
SERPINB1 MaxPab rabbit polyclonal antibody. Western Blot analysis of SERPINB1 expression in human liver.
Western Blot analysis of SERPINB1 expression in transfected 293T cell line (H00001992-T01) by SERPINB1 MaxPab polyclonal antibody.Lane 1: SERPINB1 transfected lysate(42.70 KDa).Lane 2: Non-transfected lysate.
SERPINB1 MaxPab polyclonal antibody. Western Blot analysis of SERPINB1 expression in human pancreas.
Western Blot analysis of SERPINB1 expression in transfected 293T cell line (H00001992-T02) by SERPINB1 MaxPab polyclonal antibody.Lane 1: SERPINB1 transfected lysate(41.69 KDa).Lane 2: Non-transfected lysate.
SERPINB1 MaxPab rabbit polyclonal antibody. Western Blot analysis of SERPINB1 expression in human liver.
Immunoprecipitation of SERPINB1 transfected lysate using anti-SERPINB1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SERPINB1 MaxPab mouse polyclonal antibody (B01) (H00001992-B01).
Human Stomach; WB Suggested Anti-SERPINB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Stomach; SERPINB1 antibody - middle region (ARP52417_P050) in Human Stomach cells using Western Blot
Western blot analysis of SERPINB1 Antibody (Center) Cat.-No AP53861PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the SERPINB1 antibody detected the SERPINB1 protein (arrow).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800076)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800076)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800076)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800076)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800076)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800076)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800076)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800076)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800076)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SERPINB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB1 (RC202138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB1.
HEK293T cells transfected with either RC202138 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SERPINB1 antibody(TA800076), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-SERPINB1 antibody(TA800076),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-SERPINB1 mouse monoclonal antibody (TA800077) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SERPINB1(RC202138).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB1 (RC202138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC202138 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SERPINB1 antibody(TA800077), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-SERPINB1 antibody(TA800077),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800078)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800078)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800078)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800078)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800078)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800078)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800078)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800078)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB1 (RC202138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SERPINB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC202138 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SERPINB1 antibody(TA800078), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800093)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800093)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800093)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800093)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800093)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800093)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800093)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB1 (RC202138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SERPINB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow cytometric Analysis of Jurkat cells, using anti-SERPINB1 antibody(TA800093),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-SERPINB1 antibody(TA800093),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB1 (RC202138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SERPINB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB1 (RC202138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB1.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SERPINB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-SERPINB1 antibody (1µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-SERPINB1 antibody (0.5µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-SERPINB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Stomach
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA800076 | SERPINB1 antibody |
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| SERPINB1 mouse monoclonal antibody, clone OTI4B3 (formerly 4B3) | |||||||
 |
Mouse | IgG2b | OTI4B3 | Purified | Can, Hu, Rt | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +12 additional images |
Highly validated antibody!
|
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| TA800077 | SERPINB1 antibody |
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| SERPINB1 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1) | |||||||
 |
Mouse | IgG2a | OTI1D1 | Purified | Can, Hu, Mky | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA800078 | SERPINB1 antibody |
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| SERPINB1 mouse monoclonal antibody, clone OTI2B4 (formerly 2B4) | |||||||
 |
Mouse | IgG2a | OTI2B4 | Purified | Hu, Mky | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
|
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| TA800093 | SERPINB1 antibody |
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| SERPINB1 mouse monoclonal antibody, clone OTI3B4 (formerly 3B4) | |||||||
 |
Mouse | IgG2b | OTI3B4 | Purified | Can, Hu, Mky, Rt | F, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
|
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| TA327770 | SERPINB1 antibody |
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| SERPINB1 Goat Polyclonal Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA327771 | SERPINB1 (130-143) antibody |
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| SERPINB1 Goat Polyclonal Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA350405 | SERPINB1 antibody |
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| Rabbit Polyclonal Anti-SERPINB1 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA800094 | SERPINB1 antibody |
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| SERPINB1 mouse monoclonal antibody, clone OTI2D11 (formerly 2D11) | |||||||
 |
Mouse | IgG2b | OTI2D11 | Purified | Can, Hu, Mky | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA800117 | SERPINB1 antibody |
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| SERPINB1 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10) | |||||||
 |
Mouse | IgG1 | OTI2B10 | Purified | Can, Hu, Mky | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AP53861PU-N | SERPINB1 (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302138 | SERPINB1 |
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| Recombinant protein of human serpin peptidase inhibitor, clade B (ovalbumin), member 1 (SERPINB1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP760029 | SERPINB1 (full length, N-term HIS tag) |
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| Recombinant protein of human serpin peptidase inhibitor, clade B (ovalbumin), member 1 (SERPINB1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||