Home » Target » SERPINB9 antibody

SERPINB9 antibody

Principal name

SERPINB9 antibody

Alternative names for SERPINB9 antibody

PI9, PI-9, Serpin B9, Cytoplasmic antiproteinase 3, CAP-3, CAP3, Proteinase inhibitor 9

SwissProt ID

P50453 (Human)

Gene ID

5272 (SERPINB9)

Ncbi ID

9606, NP_004146.1, NP_004146, NM_004155

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of SERPINB9 antibody

PI-9 is a 42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virusinfected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves.

General readings

Jiang X, Ellison SJ, Alarid ET, Shapiro DJ.
Interplay between the levels of estrogen and estrogen receptor controls the level of the granzyme inhibitor, proteinase inhibitor 9 and susceptibility to immune surveillance by natural killer cells.
Oncogene. 2007 Jan 22
PMID: 17237823

Western Blot analysis of SERPINB9 expression in transfected 293T cell line (H00005272-T01) by SERPINB9 MaxPab polyclonal antibody.Lane 1: SERPINB9 transfected lysate(41.36 KDa).Lane 2: Non-transfected lysate.

H00005272-B01

SERPINB9 polyclonal antibody ( Cat # PAB7241 ) ( 0.5 µg/ml ) staining of human placenta lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB7241

Immunoperoxidase of monoclonal antibody to SERPINB9 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]

H00005272-M06

Western blot analysis using recombinant SERPINB9.Lane 1 : 2 µg.Lane 2 : 5 µg.Lane 3 : 10 µg.

MAB1775

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SERPINB9 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COLO/HuvEc/Jurkat cells, using SERPINB9 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of Serpin B9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of Serpin B9 expression in Ramos (A); HuvEc (B) whole cell lysates.

TP300645

TA303212 (0.5µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA303212

Western blot analysis of extracts from Jurkat cells, using SERPINB9 antibody.The lane on the right is treated with the synthesized peptide.

TA312970

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SERPINB9 antibody.The picture on the right is treated with the synthesized peptide.

TA312970

Western blot analysis of recombinant PI9: Lane 1: 2 µg Lane 2: 5 µg Lane 3: 10 µg

TA318921

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA303212	SERPINB9 antibody
	Goat Polyclonal Antibody against SERPINB9
	Goat			Aff - Purified	Hu, Ms, Rt	WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA312970	SERPINB9 antibody
	Rabbit polyclonal anti-SERPINB9 antibody
	Rabbit	IgG		Purified	Hu, Ms	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA318921	SERPINB9 antibody
	Mouse monoclonal anti-Proteinase Inhibitor (PI-9) antibody, clone PI9-17
	Mouse	IgG1	PI9-17	Purified	Hu	P, WB	0.1 mg / €415.00
							OriGene Technologies, Inc.
AM01199PU-N	SERPINB9 antibody
	Mouse	IgG1	7D8	Purified	Hu	F, P, WB	0.1 mg / €330.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP300645	SERPINB9
	Recombinant protein of human serpin peptidase inhibitor, clade B (ovalbumin), member 9 (SERPINB9)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Antibodies (see all»)

Kits (see all»)

Antigens

Lysates (see all»)

Tissue Slides (see all»)

Other life-science products