SETD1B antibody
Principal name
SETD1B antibody
Alternative names for SETD1B antibody
SET1B, KMT2G, KIAA1076, Histone-lysine N-methyltransferase SETD1B, SET domain-containing protein 1B, Lysine N-methyltransferase 2G
Gene ID
23067 (SETD1B)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB)
Background of SETD1B antibody
- Kit Component:
- - KN227091G1, SETD1B gRNA vector 1 in pCas-Guide vector
- KN227091G2, SETD1B gRNA vector 2 in pCas-Guide vector
- KN227091D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
ELISA was performed using a serial dilution of Setd1a polyclonal antibody ( Cat # PAB14083 ). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 2,500.
WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Setd1b, diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Setd1b. The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,400.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA347285 | SETD1B antibody |
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| Rabbit Polyclonal Setd1b Antibody | |||||||
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Rabbit | IgG | Purified | Ms | E, WB |
0.1 ml /
€285.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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