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SETDB1 antibody

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Principal name

SETDB1 antibody

Alternative names for SETDB1 antibody

ESET, KMT1E, KIAA0067, Histone-lysine N-methyltransferase SETDB1, SET domain bifurcated 1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, Lysine N-methyltransferase 1E

SwissProt ID

O88974 (Mouse), Q15047 (Human), Q32KD2 (Drome), Q6INA9 (Xenla)

Gene ID

9869 (SETDB1)

Ncbi ID

9606, NP_001138887.1, 10090, NM_012432, NM_001145415, NP_036564, NP_001138887

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of SETDB1 antibody

The SET domain is a highly conserved,
approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger
conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is
derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of
condensed chromatin. Although ESET is a widely expressed protein, it is highly expressed in the testis.

AP07866PU-N - SETDB1
Figure 1. Staining SETDB1 in Testis by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
H00009869-M07 - SETDB1
Immunofluorescence of monoclonal antibody to SETDB1 on HeLa cell. [antibody concentration 10 ug/ml]
H00009869-M07 - SETDB1
SETDB1 monoclonal antibody (M07), clone 4A3 Western Blot analysis of SETDB1 expression in SW-13 ( Cat # L005V1 ).
H00009869-M07 - SETDB1
Detection limit for recombinant GST tagged SETDB1 is approximately 0.1ng/ml as a capture antibody.
H00009869-B01 - SETDB1
Western Blot analysis of SETDB1 expression in transfected 293T cell line (H00009869-T01) by SETDB1 MaxPab polyclonal antibody.Lane 1: SETDB1 transfected lysate(141.9 KDa).Lane 2: Non-transfected lysate.
AP11091PU-N - SETDB1
LEFT: Western blot analysis of anti-SETDB1 Pab(Cat. #AP11091PU-N) in Ramos cell line lysate (35ug/lane). SETDB1 (arrow) was detected using the purified Pab (1:60 dilution). RIGHT: Western blot analysis of SETDB1(arrow) using rabbit polyclonal SETDB1 Antibody (C-term) (Cat.#AP11091PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2) (Origene Technologies).
PAB10044 - SETDB1
Western blot analysis is shown using SETDB1 polyclonal antibody ( Cat # PAB10044 ) to detect human SETBD1 present in a 293 whole cell lysate.Approximately 30 µg of lysate was loaded per lane for SDS-PAGE.Comparison to a molecular weight marker ( not shown ) indicates a singleband of ~170 kDa is detected.Peptide competition ( not shown ) blocks staining of this band.The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2h followed by detection using IRDyeTM800 labeled Goat-a-Rabbit IgG [H&L] diluted1:5,000 for 45 min.IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
AM06545SU-N - SETDB1
Western blot analysis using SETDB1 antibody Cat.-No AM06545SU-N against MCF-7 (1), T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5) and F9 (6) cell lysate.
AM06546SU-N - SETDB1
Immunofluorescence analysis of LOVO cells using SETDB1 antibody Cat.-No AM06546SU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
AM06546SU-N - SETDB1
Western blot analysis using SETDB1 antibody Cat.-No AM06546SU-N against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5), F9 (6), RAW246.7 (7) and Cos7 (8) cell lysate.
TP303647 - SETDB1
TP326620 - SETDB1
TA319369 - SETDB1
WB analysis using Anti-ESET antibody to detect human ESET present in a 293 whole cell lysate.   ~30µg of lysate was loaded per lane for SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected.  Peptide competition (not shown) blocks staining of this band.  The blot was incubated with a 1:1000 dilution of the antibody at RT for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.
TA332979 - SETDB1
Western blot analysis of extracts of various cell lines, using SETDB1 antibody.

2 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SETDB1 antibody
Western blot analysis using SETDB1 antibody Cat.-No AM06545SU-N against MCF-7 (1), T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5) and F9 (6) cell lysate. Mouse IgG1 5H6D4 Ascites Hu, Mky, Ms E, WB
0.1 ml / €380.00
  OriGene Technologies GmbH

SETDB1 antibody
Immunofluorescence analysis of LOVO cells using SETDB1 antibody Cat.-No AM06546SU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 5H6A12 Ascites Hu, Mky, Ms E, ICC/IF, WB
0.1 ml / €380.00
  OriGene Technologies GmbH
+1 additional image

2 Item(s)

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Available Filters

Product Type
  1. Primary Antibody (2)
Clonality
  1. Monoclonal (2)
Target
  1. SETDB1 (2)
Reactivity
  1. Human (Hu) (2)
  2. Monkey (Mky) (2)
  3. Mouse (Ms) (2)
Applications
  1. Enzyme Immunoassay (E) (2)
  2. Western blot / Immunoblot (WB) (2)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Ascites (2)
Images
  1. 1 image (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Manufacturer
  1. OriGene Technologies (2)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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  • orders: ordering-de@origene.com
 
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