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SETDB1 antibody

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Principal name

SETDB1 antibody

Alternative names for SETDB1 antibody

ESET, KMT1E, KIAA0067, Histone-lysine N-methyltransferase SETDB1, SET domain bifurcated 1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, Lysine N-methyltransferase 1E

SwissProt ID

O88974 (Mouse), Q15047 (Human), Q32KD2 (Drome), Q6INA9 (Xenla)

Gene ID

9869 (SETDB1)

Ncbi ID

9606, NP_001138887.1, 10090, NM_012432, NM_001145415, NP_036564, NP_001138887

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of SETDB1 antibody

The SET domain is a highly conserved,
approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger
conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is
derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of
condensed chromatin. Although ESET is a widely expressed protein, it is highly expressed in the testis.

AP07866PU-N - SETDB1
Figure 1. Staining SETDB1 in Testis by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
H00009869-M07 - SETDB1
Immunofluorescence of monoclonal antibody to SETDB1 on HeLa cell. [antibody concentration 10 ug/ml]
H00009869-M07 - SETDB1
SETDB1 monoclonal antibody (M07), clone 4A3 Western Blot analysis of SETDB1 expression in SW-13 ( Cat # L005V1 ).
H00009869-M07 - SETDB1
Detection limit for recombinant GST tagged SETDB1 is approximately 0.1ng/ml as a capture antibody.
H00009869-B01 - SETDB1
Western Blot analysis of SETDB1 expression in transfected 293T cell line (H00009869-T01) by SETDB1 MaxPab polyclonal antibody.Lane 1: SETDB1 transfected lysate(141.9 KDa).Lane 2: Non-transfected lysate.
AP11091PU-N - SETDB1
LEFT: Western blot analysis of anti-SETDB1 Pab(Cat. #AP11091PU-N) in Ramos cell line lysate (35ug/lane). SETDB1 (arrow) was detected using the purified Pab (1:60 dilution). RIGHT: Western blot analysis of SETDB1(arrow) using rabbit polyclonal SETDB1 Antibody (C-term) (Cat.#AP11091PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2) (Origene Technologies).
PAB10044 - SETDB1
Western blot analysis is shown using SETDB1 polyclonal antibody ( Cat # PAB10044 ) to detect human SETBD1 present in a 293 whole cell lysate.Approximately 30 µg of lysate was loaded per lane for SDS-PAGE.Comparison to a molecular weight marker ( not shown ) indicates a singleband of ~170 kDa is detected.Peptide competition ( not shown ) blocks staining of this band.The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2h followed by detection using IRDyeTM800 labeled Goat-a-Rabbit IgG [H&L] diluted1:5,000 for 45 min.IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
AM06545SU-N - SETDB1
Western blot analysis using SETDB1 antibody Cat.-No AM06545SU-N against MCF-7 (1), T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5) and F9 (6) cell lysate.
AM06546SU-N - SETDB1
Immunofluorescence analysis of LOVO cells using SETDB1 antibody Cat.-No AM06546SU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
AM06546SU-N - SETDB1
Western blot analysis using SETDB1 antibody Cat.-No AM06546SU-N against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5), F9 (6), RAW246.7 (7) and Cos7 (8) cell lysate.
TP303647 - SETDB1
TP326620 - SETDB1
TA319369 - SETDB1
WB analysis using Anti-ESET antibody to detect human ESET present in a 293 whole cell lysate.   ~30µg of lysate was loaded per lane for SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected.  Peptide competition (not shown) blocks staining of this band.  The blot was incubated with a 1:1000 dilution of the antibody at RT for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.
TA332979 - SETDB1
Western blot analysis of extracts of various cell lines, using SETDB1 antibody.

4 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SETDB1 antibody
  Rabbit polyclonal anti-ESET antibody  
WB analysis using Anti-ESET antibody to detect human ESET present in a 293 whole cell lysate.   ~30µg of lysate was loaded per lane for SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected.  Peptide competition (not shown) blocks staining of this band.  The blot was incubated with a 1:1000 dilution of the antibody at RT for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min. Rabbit Aff - Purified Hu E, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.

SETDB1 antibody
  Rabbit anti-SETDB1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using SETDB1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.

SETDB1 antibody
Figure 1. Staining SETDB1 in Testis by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Hu E, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

SETDB1 (C-term) antibody
LEFT: Western blot analysis of anti-SETDB1 Pab(Cat. #AP11091PU-N) in Ramos cell line lysate (35ug/lane). SETDB1 (arrow) was detected using the purified Pab (1:60 dilution). RIGHT: Western blot analysis of SETDB1(arrow) using rabbit polyclonal SETDB1 Antibody (C-term) (Cat.#AP11091PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

4 Item(s)

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Product Type
  1. Primary Antibody (4)
Target
  1. SETDB1 (4)
Property
  1. C-term (1)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (1)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (2)
  3. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (2)
  2. Purified (2)
Host
  1. Rabbit (4)
Images
  1. 1 image (4)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (4)
Service
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