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SF3B3 / SAP130 antibody

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Principal name

SF3B3 / SAP130 antibody

Alternative names for SF3B3 / SAP130 antibody

Splicing factor 3B subunit 3, SF3b130, SAP-130, SAP 130, STAF130, KIAA0017, Spliceosome-associated protein 130, Pre-mRNA-splicing factor SF3b 130 kDa subunit

SwissProt ID

A0JN52 (Bovin), Q15393 (Human), Q1LVE8 (Danre), Q921M3 (Mouse)

Gene ID

23450 (SF3B3), 101943 (Sf3b3)

Ncbi ID

9606, NP_036558, NP_036558.3, NP_598714.1

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of SF3B3 / SAP130 antibody

SF3B3 is subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron\'s branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron\'s branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R.
Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155.
Mol Cell Biol 1999 Oct;19(10):6796-802
PMID: 10490618

PAB6215 - SF3B3 / SAP130
SF3B3 polyclonal antibody ( Cat # PAB6215 ) staining ( 0.1 µg/ml ) of nuclear lysate from HeLa cells ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ARP40731_P050 - SF3B3 / SAP130
Transfected 293T; WB Suggested Anti-SF3B3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; SF3B3 antibody - middle region (ARP40731_P050) in Transfected 293T cells using Western Blot
AP53870PU-N - SF3B3 / SAP130
Western blot analysis using SF3B3 Antibody (C-term) Cat.-No AP53870PU-N in CEM cell line lysates (35ug/lane). This demonstrates the SF3B3 antibody detected the SF3B3 protein (arrow).
CPA2574-100ul - SF3B3 / SAP130
Western blot analysis of SAP130 expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates.
TP309509 - SF3B3 / SAP130
TA302598 - SF3B3 / SAP130
TA302598 staining (0.1µg/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA308399 - SF3B3 / SAP130
SAP130 antibody [C3], C-term detects SAP130 protein by western blot analysis. A. 30 ug HeLa whole cell extract. 5 % SDS-PAGE. SAP130 antibody [C3], C-term (TA308399) dilution: 1:10000
TA308399 - SF3B3 / SAP130
Immunohistochemical analysis of paraffin-embedded Saos-2 xenograft, using SF3B3 (TA308399) antibody at 1:100 dilution.
TA308399 - SF3B3 / SAP130
Immunofluorescence analysis of methanol-fixed HeLa, using SAP130(TA308399) antibody at 1:500 dilution.
TA343784 - SF3B3 / SAP130
WB Suggested Anti-SF3B3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HCT15 cell lysate; SF3B3 is supported by BioGPS gene expression data to be expressed in HCT15
TA343785 - SF3B3 / SAP130
WB Suggested Anti-SF3B3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Transfected 293T

4 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SF3B3 / SAP130 (C-term) antibody
  Goat Polyclonal Antibody against SAP130 / SF3B3  
TA302598 staining (0.1µg/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

SF3B3 / SAP130 antibody
  Rabbit polyclonal antibody to SAP130 (splicing factor 3b, subunit 3, 130kDa)  
SAP130 antibody [C3], C-term detects SAP130 protein by western blot analysis. A. 30 ug HeLa whole cell extract. 5 % SDS-PAGE. SAP130 antibody [C3], C-term (TA308399) dilution: 1:10000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

SF3B3 / SAP130 (C-term) antibody
Western blot analysis using SF3B3 Antibody (C-term) Cat.-No AP53870PU-N in CEM cell line lysates (35ug/lane). This demonstrates the SF3B3 antibody detected the SF3B3 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SF3B3 / SAP130
  Recombinant protein of human splicing factor 3b, subunit 3, 130kDa (SF3B3)  
SF3B3 / SAP130 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.

4 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (3)
  2. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (3)
Target
  1. SF3B3 / SAP130 (4)
Property
  1. C-term (2)
Reactivity
  1. Human (Hu) (3)
  2. Bovine (Bov) (1)
  3. Canine (Can) (1)
  4. Mouse (Ms) (1)
  5. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (3)
Host
  1. Rabbit (2)
  2. Goat (1)
Images
  1. 1 image (4)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Immunofluorescence (IF) (1)
  3. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (4)
Service
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