SGK196 antibody
Principal name
SGK196 antibody
Alternative names for SGK196 antibody
Sugen kinase 196, FLJ23356, Protein kinase-like protein SgK196
Ncbi ID
9606, NP_115613, NP_115613.1, NM_032237, NM_029037, NM_001024883, NP_083313.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)
Background of SGK196 antibody
- Kit Component:
- - KN313627G1, 4930444A02Rik gRNA vector 1 in pCas-Guide vector
- KN313627G2, 4930444A02Rik gRNA vector 2 in pCas-Guide vector
- KN313627D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Aruga,J., (2005) Science 307 (5715), 1621-1625
Immunofluorescence of monoclonal antibody to FLJ23356 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot analysis of FLJ23356 over-expressed 293 cell line, cotransfected with FLJ23356 Validated Chimera RNAi ( Cat # H00084197-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FLJ23356 monoclonal antibody (M03), clone 6F10 (Cat # H00084197-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of FLJ23356 expression in transfected 293T cell line by FLJ23356 monoclonal antibody (M03), clone 6F10.Lane 1: FLJ23356 transfected lysate(38.5 KDa).Lane 2: Non-transfected lysate.
FLJ23356 monoclonal antibody (M03), clone 6F10. Western Blot analysis of FLJ23356 expression in HeLa(Cat # L013V1 ).
FLJ23356 monoclonal antibody (M03), clone 6F10. Western Blot analysis of FLJ23356 expression in HepG2(Cat # L019V1 ).
Detection limit for recombinant GST tagged FLJ23356 is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of SGK196 expression in transfected 293T cell line (H00084197-T01) by SGK196 MaxPab polyclonal antibody.Lane 1: FLJ23356 transfected lysate(38.5 KDa).Lane 2: Non-transfected lysate.
SGK196 MaxPab rabbit polyclonal antibody. Western Blot analysis of SGK196 expression in mouse liver.
Western Blot analysis of SGK196 expression in transfected 293T cell line (H00084197-T01) by SGK196 MaxPab polyclonal antibody.Lane 1: FLJ23356 transfected lysate(40.00 KDa).Lane 2: Non-transfected lysate.
Mouse renal epithelial; . Lanes: Lane 1: 30ug mouse renal epithelial lysate. Lane 2: 30ug mouse renal epithelial lysate. Lane 3: 30ug mouse renal epithelial lysate. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:2500. Gene Name: FLJ23356. Submitted by: Dr. JUNMING FAN,ECU.; FLJ23356 antibody - N-terminal region (ARP50048_P050) in Mouse renal epithelial cells using Western Blot
Human 721_B; WB Suggested Anti-FLJ23356 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate; FLJ23356 antibody - N-terminal region (ARP50048_P050) in Human 721_B cells using Western Blot
SGK196 MaxPab rabbit polyclonal antibody. Western Blot analysis of SGK196 expression in mouse liver.
Immunoprecipitation of SGK196 transfected lysate using anti-SGK196 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SGK196 MaxPab mouse polyclonal antibody (B01) (H00084197-B01).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SGK196 (RC222797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SGK196.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SGK196 Mouse monoclonal antibody. (TA804622; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SGK196 (RC222797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SGK196.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SGK196 Mouse monoclonal antibody. (TA804623; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SGK196 (RC222797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SGK196.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SGK196 (RC222797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SGK196.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SGK196 (RC222797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SGK196.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SGK196 (RC222797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SGK196.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SGK196 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804649)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SGK196 (RC222797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SGK196.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SGK196 (RC222797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SGK196.
Lanes: Lane 1: 30ug mouse renal epithelial lysate; Lane 2: 30ug mouse renal epithelial lysate; Lane 3: 30ug mouse renal epithelial lysate; Primary Antibody Dilution: 1: 500; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1: 2500; Gene N
WB Suggested Anti-SGK196 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 721_B cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA804574 | SGK196 antibody |
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| SGK196 mouse monoclonal antibody, clone OTI3F9 (formerly 3F9) | |||||||
 |
Mouse | IgG1 | OTI3F9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA804622 | SGK196 antibody |
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| SGK196 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10) | |||||||
 |
Mouse | IgG1 | OTI2B10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|
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| TA804623 | SGK196 antibody |
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| SGK196 mouse monoclonal antibody, clone OTI1B1 (formerly 1B1) | |||||||
 |
Mouse | IgG2a | OTI1B1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA804625 | SGK196 antibody |
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| SGK196 mouse monoclonal antibody, clone OTI2B6 (formerly 2B6) | |||||||
 |
Mouse | IgG2b | OTI2B6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA804646 | SGK196 antibody |
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| SGK196 mouse monoclonal antibody, clone OTI4G2 (formerly 4G2) | |||||||
 |
Mouse | IgG1 | OTI4G2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA804648 | SGK196 antibody |
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| SGK196 mouse monoclonal antibody, clone OTI4F6 (formerly 4F6) | |||||||
 |
Mouse | IgG2a | OTI4F6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA804649 | SGK196 antibody |
||||||
| SGK196 mouse monoclonal antibody, clone OTI4D9 (formerly 4D9) | |||||||
 |
Mouse | IgG1 | OTI4D9 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA804718 | SGK196 antibody |
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| SGK196 mouse monoclonal antibody, clone OTI2E6 (formerly 2E6) | |||||||
 |
Mouse | IgG1 | OTI2E6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP322797 | SGK196 |
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| Recombinant protein of human protein kinase-like protein SgK196 (SGK196) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY410261 | POMK overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205284L2V | Lenti ORF particles, 4930444A02Rik (GFP-tagged) - Mouse RIKEN cDNA 4930444A02 gene (4930444A02Rik), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205284L1V | Lenti ORF particles, 4930444A02Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930444A02 gene (4930444A02Rik), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR213415L2V | Lenti ORF particles, Sgk196 (GFP-tagged ORF) - Rat similar to RIKEN cDNA 4930444A02 (RGD1310810), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR213415L1V | Lenti ORF particles, Sgk196 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 4930444A02 (RGD1310810), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
