SH2B3 antibody
Principal name
SH2B3 antibody
Alternative names for SH2B3 antibody
SH2B3, LNK, SH2B adapter protein 3, Lymphocyte-specific adapter protein Lnk, Signal transduction protein Lnk, Lymphocyte adapter protein
Ncbi ID
NP_005466.1, 9606, NP_005466, NP_032533.1, NM_008507, NM_031621, NM_005475
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of SH2B3 antibody
- Kit Component:
- - KN315698G1, Sh2b3 gRNA vector 1 in pCas-Guide vector
- KN315698G2, Sh2b3 gRNA vector 2 in pCas-Guide vector
- KN315698D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Li Y, He X, Schembri-King J, Jakes S, Hayashi J. Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation. J Immunol. 2000 May 15;164(10):5199-206.
HEK293 overexpressing SH2B3 and probed with SH2B3 polyclonal antibody ( Cat # PAB6669 ) ( mock transfection in first lane ), tested by Origene.
SH2B3 polyclonal antibody ( Cat # PAB6669 ) ( 0.3 µg/ml ) staining of MOLT 4 lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of LNK expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2B3 (RC218359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2B3.
HEK293T cells transfected with either RC218359 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2B3 antibody(TA502747), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-SH2B3 antibody(TA502747),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-SH2B3 mouse monoclonal antibody (TA502748) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2B3(RC218359 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2B3 (RC218359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2B3.
HEK293T cells transfected with either RC218359 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2B3 antibody(TA502748), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-SH2B3 antibody(TA502748),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-SH2B3 antibody(TA502748),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-SH2B3 mouse monoclonal antibody (TA502762) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2B3(RC218359 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2B3 (RC218359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2B3.
HEK293T cells transfected with either RC218359 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2B3 antibody(TA502762), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SH2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502764)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SH2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502764)
Anti-SH2B3 mouse monoclonal antibody (TA502764) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2B3(RC218359 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2B3 (RC218359 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2B3.
Flow cytometric Analysis of Hela cells, using anti-SH2B3 antibody(TA502764),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC218359 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2B3 antibody(TA502764), and then analyzed by flow cytometry.
Anti-SH2B3 mouse monoclonal antibody (TA502933) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2B3(RC218359 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2B3 (RC218359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2B3.
HEK293T cells transfected with either RC218359 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2B3 antibody(TA502933), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-SH2B3 antibody(TA502933),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-SH2B3 antibody(TA502933),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-SH2B3 mouse monoclonal antibody (TA503069) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2B3(RC218359 ).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2B3 (RC218359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2B3.
HEK293T cells transfected with either RC218359 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2B3 antibody(TA503069), and then analyzed by flow cytometry.
TA302531 (0.3µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502748 | SH2B3 antibody |
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| SH2B3 (LNK) mouse monoclonal antibody, clone OTI2B11 (formerly 2B11) | |||||||
 |
Mouse | IgG2a | OTI2B11 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody!
|
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| TA502764 | SH2B3 antibody |
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| SH2B3 (LNK) mouse monoclonal antibody, clone OTI2D8 (formerly 2D8) | |||||||
 |
Mouse | IgG1 | OTI2D8 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
||||||
| TA502933 | SH2B3 antibody |
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| SH2B3 (LNK) mouse monoclonal antibody, clone OTI2C9 (formerly 2C9) | |||||||
 |
Mouse | IgG1 | OTI2C9 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
||||||
| TA302531 | SH2B3 antibody |
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| Goat Polyclonal Antibody against LNK / SH2B3 | |||||||
 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA502747 | SH2B3 antibody |
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| SH2B3 (LNK) mouse monoclonal antibody, clone OTI6F11 (formerly 6F11) | |||||||
 |
Mouse | IgG1 | OTI6F11 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA502762 | SH2B3 antibody |
||||||
| SH2B3 (LNK) mouse monoclonal antibody, clone OTI1E6 (formerly 1E6) | |||||||
 |
Mouse | IgG1 | OTI1E6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA503069 | SH2B3 antibody |
||||||
| SH2B3 (LNK) mouse monoclonal antibody, clone OTI1F6 (formerly 1F6) | |||||||
 |
Mouse | IgG1 | OTI1F6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| AP05552PU-N | SH2B3 antibody |
||||||
 |
Goat | IgG | Purified | Hu | WB |
0.1 mg /
€590.00
|
|
| OriGene Technologies GmbH | |||||||
| AP55254PU-N | SH2B3 antibody |
||||||
|
Rabbit | Purified | Hu, Mky, Ms, Rt | C, E, WB |
0.1 mg /
€450.00
|
||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP318359 | SH2B3 |
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| Recombinant protein of human SH2B adaptor protein 3 (SH2B3) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR208726L2V | Lenti ORF particles, Sh2b3 (GFP-tagged) - Mouse SH2B adaptor protein 3 (Sh2b3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,250.00
|
|
| OriGene Technologies, Inc. | ||
| MR208726L1V | Lenti ORF particles, Sh2b3 (Myc-DDK-tagged) - Mouse SH2B adaptor protein 3 (Sh2b3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,120.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR208542L2V | Lenti ORF particles, Sh2b3 (GFP-tagged ORF) - Rat SH2B adaptor protein 3 (Sh2b3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR208542L1V | Lenti ORF particles, Sh2b3 (Myc-DDK-tagged ORF) - Rat SH2B adaptor protein 3 (Sh2b3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||