SH2D2A antibody
Principal name
SH2D2A antibody
Alternative names for SH2D2A antibody
SCAP, TSAD, VRAP, SH2 domain-containing protein 2A, SH2 domain-containing adapter protein, T cell-specific adapter protein, VEGF receptor-associated protein
Ncbi ID
9606, NP_003966, NP_001154914.1, AAH12107, NM_003975, NM_001161442, NM_001161444, NM_001025571, NM_001161441, NM_001161443
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of SH2D2A antibody
This gene encodes an adaptor protein thought to function in T-cell sigl transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kise and functions in downstream sigling. Altertive splicing results in multiple transcript variants. [provided by RefSeq].
Western Blot analysis of SH2D2A expression in transfected 293T cell line (H00009047-T01) by SH2D2A MaxPab polyclonal antibody.Lane 1: SH2D2A transfected lysate(42.79 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SH2D2A expression in transfected 293T cell line (H00009047-T01) by SH2D2A MaxPab polyclonal antibody.Lane 1: SH2D2A transfected lysate(42.79 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SH2D2A expression in transfected 293T cell line (H00009047-T02) by SH2D2A MaxPab polyclonal antibody.Lane 1: SH2D2A transfected lysate(42.79 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SH2D2A expression in transfected 293T cell line (H00009047-T03) by SH2D2A MaxPab polyclonal antibody.Lane 1: SH2D2A transfected lysate(42.90 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of SH2D2A transfected lysate using anti-SH2D2A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SH2D2A MaxPab mouse polyclonal antibody (B02) (H00009047-B02).
HEK293T cells were transfected with SH2D2A cDNA. After 48 hours, the cells were detached and fixed with 2% formaldehyde for 30 min, then permeabilized with 0.1% trixon-X100. The cells were then stained with Dylight488 conjugated mouse IgG1 (black line) or Dylight488 conjugated SH2D2A antibodies (red line).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Figure from citation: Comparison of IHC on tonsil with clone 4F3(TA504364) and clone 3C7(TA504372) SH2D2A mAb and HRP-conjugated secondary antibodies. View Citation
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SH2D2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504364)
Anti-SH2D2A mouse monoclonal antibody (TA504364) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2D2A(RC204162).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (RC204162, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.
HEK293T cells transfected with either RC204162 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2D2A antibody(TA504364), and then analyzed by flow cytometry.
Figure from citation: Comparison of IHC on tonsil with clone 4F3(TA504364) and clone 3C7(TA504372) SH2D2A mAb and HRP-conjugated secondary antibodies. View Citation
Anti-SH2D2A mouse monoclonal antibody (TA504372) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2D2A(RC204162).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SH2D2A monoclonal antibody.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SH2D2A monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (RC204162, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.
HEK293T cells transfected with either RC204162 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2D2A antibody(TA504372), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (RC204162, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.
HEK293T cells transfected with either RC204162 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2D2A antibody(TA504381), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (RC204162, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.
HEK293T cells transfected with either RC204162 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2D2A antibody(TA504461), and then analyzed by flow cytometry.
Western blot analysis of extracts from HepG2 cells, using SH2D2A antibody.The lane on the right is treated with the synthesized peptide.
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- Cited (3)
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA400030 | SH2D2A antibody |
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| Anti-SH2D2A mouse mAb, clone 3C7, DyLight488 , clone OTI3C7 (formerly 3C7) | |||||||
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Mouse | IgG1 | OTI3C7 | Purified | Hu, Mky, Ms | F |
0.1 ml /
€365.00
|
| OriGene Technologies, Inc. | |||||||
Published recently! |
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| TA504364 | SH2D2A antibody |
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| SH2D2A mouse monoclonal antibody, clone OTI4F3 (formerly 4F3) | |||||||
 |
Mouse | IgG1 | OTI4F3 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +11 additional images |
Published recently! Highly validated antibody!
|
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| TA504372 | SH2D2A antibody |
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| SH2D2A mouse monoclonal antibody, clone OTI3C7 (formerly 3C7) | |||||||
 |
Mouse | IgG1 | OTI3C7 | Purified | Hu, Mky, Ms | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Published recently! Highly validated antibody!
|
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| TA312991 | SH2D2A antibody |
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| Rabbit polyclonal anti-SH2D2A antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA504381 | SH2D2A antibody |
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| SH2D2A mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) | |||||||
 |
Mouse | IgG1 | OTI1B8 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA504461 | SH2D2A antibody |
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| SH2D2A mouse monoclonal antibody, clone OTI6G1 (formerly 6G1) | |||||||
 |
Mouse | IgG1 | OTI6G1 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304162 | SH2D2A |
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| Recombinant protein of human SH2 domain protein 2A (SH2D2A) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418322 | SH2D2A overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY431326 | SH2D2A overexpression lysate |
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0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. | ||
| LY431896 | SH2D2A overexpression lysate |
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0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205637L2V | Lenti ORF particles, Sh2d2a (GFP-tagged) - Mouse SH2 domain protein 2A (Sh2d2a), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205637L1V | Lenti ORF particles, Sh2d2a (Myc-DDK-tagged) - Mouse SH2 domain protein 2A (Sh2d2a), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||