SH2D4A antibody
Principal name
SH2D4A antibody
Alternative names for SH2D4A antibody
SH2A, SH2 domain-containing protein 4A, Protein SH(2)A
Ncbi ID
9606, NP_001167630.1, NP_001167630, NM_022071, NM_001174160, NM_028182, NM_001012048, NM_001174159, NP_082458.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of SH2D4A antibody
- Kit Component:
- - KN315704G1, Sh2d4a gRNA vector 1 in pCas-Guide vector
- KN315704G2, Sh2d4a gRNA vector 2 in pCas-Guide vector
- KN315704D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Lapinski PE, Oliver JA, Kamen LA, Hughes ED, Saunders TL, King PD.
Genetic analysis of SH2D4A, a novel adapter protein related to T cell-specific adapter and adapter protein in lymphocytes of unknown function, reveals a redundant
function in T cells.
J. Immunol. 2008 Aug 181 (3): 2019-27.
PMID: 18641339
Immunoperoxidase of monoclonal antibody to SH2D4A on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to SH2D4A on HeLa cell. [antibody concentration 30 ug/ml]
SH2D4A monoclonal antibody (M01), clone 3G8 Western Blot analysis of SH2D4A expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged SH2D4A is approximately 0.03ng/ml as a capture antibody.
SH2D4A MaxPab polyclonal antibody. Western Blot analysis of SH2D4A expression in human liver.
Western Blot analysis of SH2D4A expression in transfected 293T cell line (H00063898-T01) by SH2D4A MaxPab polyclonal antibody.Lane 1: SH2D4A transfected lysate(49.94 KDa).Lane 2: Non-transfected lysate.
SH2D4A MaxPab polyclonal antibody. Western Blot analysis of SH2D4A expression in human liver.
Western Blot analysis of SH2D4A expression in transfected 293T cell line (H00063898-T01) by SH2D4A MaxPab polyclonal antibody.Lane 1: SH2D4A transfected lysate(49.94 KDa).Lane 2: Non-transfected lysate.
SH2D4A MaxPab rabbit polyclonal antibody. Western Blot analysis of SH2D4A expression in human pancreas.
Western Blot analysis of SH2D4A expression in transfected 293T cell line (H00063898-T01) by SH2D4A MaxPab polyclonal antibody.Lane 1: SH2D4A transfected lysate(52.70 KDa).Lane 2: Non-transfected lysate.
SH2D4A polyclonal antibody ( Cat # PAB11462 ) ( 0.1 µg/ml ) staining of human kidney lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human kidney tissue stained with SH2D4A Antibody (N-term) Cat.-No AP53897PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SH2D4A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis using SH2D4A Antibody (N-term) Cat.-No AP53897PU-N in NCI-H292 cell line lysates (35ug/lane). This demonstrates the SH2D4A antibody detected the SH2D4A protein (arrow).
Immunohistochemical analysis of SH2D4A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of SH2D4A expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.
TA305729 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-SH2D4A Antibody; Titration: 1.0 ug/ml; Positive Control: HepG2 Whole Cell
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA305729 | SH2D4A antibody |
||||||
| Goat Anti-SH2D4A Antibody | |||||||
 |
Goat | Aff - Purified | Can, Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| AP53897PU-N | SH2D4A (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY411794 | SH2D4A overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY433023 | SH2D4A overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR216461L2V | Lenti ORF particles, Sh2d4a (GFP-tagged) - Mouse SH2 domain containing 4A (Sh2d4a), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR216461L1V | Lenti ORF particles, Sh2d4a (Myc-DDK-tagged) - Mouse SH2 domain containing 4A (Sh2d4a), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201415L2V | Lenti ORF particles, Sh2d4a (GFP-tagged ORF) - Rat SH2 domain containing 4A (Sh2d4a), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR201415L1V | Lenti ORF particles, Sh2d4a (Myc-DDK-tagged ORF) - Rat SH2 domain containing 4A (Sh2d4a), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||