SH3BGRL antibody
Principal name
SH3BGRL antibody
Alternative names for SH3BGRL antibody
SH3 domain-binding glutamic acid-rich-like protein
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))
Background of SH3BGRL antibody
- Kit Component:
- - KN315709G1, Sh3bgrl gRNA vector 1 in pCas-Guide vector
- KN315709G2, Sh3bgrl gRNA vector 2 in pCas-Guide vector
- KN315709D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Egeo A., et al. (1998) Biochem. Biophys. Res. Commun. 247:302-306Yin L., et al. (2006) Proteins. 61:213-216
Detection limit for recombinant GST tagged SH3BGRL is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of SH3BGRL expression in transfected 293T cell line (H00006451-T01) by SH3BGRL MaxPab polyclonal antibody.Lane1:SH3BGRL transfected lysate(12.65 KDa).Lane 2:Non-transfected lysate.
Human Pineal Tissue; Rabbit Anti-SH3BGRL Antibody . Catalog Number: ARP48218_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue . Observed Staining: Cytoplasmic in cell bodies of pinealocytes . Primary Antibody Concentration: 1:100 . Other Working Concentrations: 1/600 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-SH3BGRL Antibody (ARP48218_P050) ) in Human Pineal Tissue using Immunohistochemistry
Human MCF7; WB Suggested Anti-SH3BGRL Antibody Titration: 0.2-1 ug/ml. Positive Control: MCF7 cell lysate. SH3BGRL is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.; SH3BGRL antibody - middle region (ARP48218_P050) in Human MCF7 cells using Western Blot
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304993 | SH3BGRL |
||||
| Recombinant protein of human SH3 domain binding glutamic acid-rich protein like (SH3BGRL) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR200469L2V | Lenti ORF particles, Sh3bgrl (GFP-tagged) - Mouse SH3-binding domain glutamic acid-rich protein like (Sh3bgrl), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR200469L1V | Lenti ORF particles, Sh3bgrl (Myc-DDK-tagged) - Mouse SH3-binding domain glutamic acid-rich protein like (Sh3bgrl), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||